MEM - sterile-filtered, for cell culture, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid

Cat. No.: M755909

GRADE & PURITY Sterile-filtered, for Cell culture, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.

MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.

Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Application:

Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.

Specifications

Specifications & Purity

sterile-filtered, for cell culture, With Earle′s salts,  stable glutamine, and sodium bicarbonate, liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

for Cell culture, Sterile-filtered

References

1. Sirui Zhang, Jingdan Wang, Rong Yu, Haiping Liu, Shuyan Liu, Kai Luo, Jin'e Lei, Bei Han, Yanjiong Chen, Shaoshan Han, E Yang, Meng Xun, Lei Han.  (2024)  The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in Acinetobacter baumannii.  Current Research in Microbial Sciences,      [PMID:39758053] [10.1016/j.crmicr.2024.100332]