Penicillinase, CAS No.9001-74-5

CAS: 9001-74-5 Cat. No.: P1508160 EC Number: 232-628-8

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, Sterile, expressed in E.coli;≥10 M units/mL

Accession #

P00809

Expression system

E. coli

Bioactivity

≥10 M units/mL

Overview

Penicillinase, also known as type I β-lactamase (EC 3.5.2.6), can hydrolyze the β-lactam ring of β-lactam antibiotics, thereby abrogating their antimicrobial activity. This product is a recombinant penicillinase produced by genetic engineering technology. A high-expression engineering strain of penicillinase is constructed, followed by fermentation and purification to obtain a product with high purity and high activity. The product features strong specificity. It can effectively hydrolyze β-lactam antibiotics, including benzylpenicillin (penicillin G), ampicillin, amoxicillin, pivampicillin, carbenicillin, ticarcillin, furbenicillin, mezlocillin, etc.

Scope of Application

It can be used for the sterility test and microbial limit test of β-lactam antibiotic raw materials and their preparations, namely the sterility test for β-lactam antibiotics specified in Pharmacopoeia of the People's Republic of China (2020 Edition, Volume 4, Appendix: Sterility Test) and the microbial limit test specified in the corresponding appendix. It is also used as a reagent for the sterility test specified in USP-NF General Chapters 71 STERILITY TEST.

Usage Instructions

1. Membrane Filtration Method

① Dilute 1 ml of penicillinase to 1000 ml with sterile water to prepare the washing solution. Sterilize all the equipment to be used by an appropriate method and soak them in the washing solution for 10 minutes.

② Take the prescribed amount of the test sample, filter it according to the specified method, and rinse the filter membrane with an appropriate volume of the washing solution prepared in step ①.

③ Separately dilute 1 ml of penicillinase to 200 ml with sterile water. Add 10 ml of this solution to each culture medium, and store the remaining solution at 2–8 °C for later use.

④ Inoculate the filter membrane into the culture medium according to the specified method.

2. Direct Inoculation Method

Take the prescribed amount of the test sample (in accordance with the pharmacopoeia), mix it well, add an appropriate volume of sterile penicillinase solution (in accordance with the pharmacopoeia), and inoculate the mixture into each tube of culture medium. Alternatively, directly inoculate the test sample into the culture medium containing sterile penicillinase.

Specifications

Product Name

Penicillinase, CAS No.9001-74-5

Synonyms

β-lactamase type I | β-Lactamase I

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, Sterile, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, sterile, expressed in E.coli;≥10 M units/mL

Biochemical and Physiological Mechanisms

This protein is a beta-lactamase with a substrate specificity for penicillins. The class A beta-lactamase family has a specific amino-acid numbering system, sometimes called Ambler or ABL numbering and often misspelt as Amber. A multiple sequence alignme

Bioactivity

≥10 M units/mL

Accession #

P00809

CAS

9001-74-5

Enzyme Commission Number

EC 3.5.2.6

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli;≥10 M units/mL

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Unit definition

One activity unit is defined as the amount of enzyme required to hydrolyze 1 unit of penicillin G in 1 hour under conditions of pH 7.0 and 37˚C.

References

1. Wenyao Chen, Shan Shan, Juan Peng, Daofeng Liu, Jun Xia, Bing Shao, Weihua Lai.  (2020)  Sensitive and hook effect–free lateral flow assay integrated with cascade signal transduction system.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2020.128465]

2. Shan Shan, Daofeng Liu, Qi Guo, Songsong Wu, Rui Chen, Kai Luo, Liming Hu, Yonghua Xiong, Weihua Lai.  (2016)  Sensitive detection of Escherichia coli O157:H7 based on cascade signal amplification in ELISA.  JOURNAL OF DAIRY SCIENCE,      [PMID:27394946] [10.3168/jds.2016-11320]

3. Chen Chen, Jun Lv, Wanyi Chen, Yang Xiang, Juan Zhang.  (2016)  Colorimetric β-lactamase inhibitor assay with double catalyzed signal amplification.  NEW JOURNAL OF CHEMISTRY,  40  (3): (1982-1987).  [PMID:] [10.1039/C5NJ02356D]

4. Lihong Li, Zhao Li, Wen Shi, Xiaohua Li, Huimin Ma.  (2014)  Sensitive and Selective Near-Infrared Fluorescent Off–On Probe and Its Application to Imaging Different Levels of β-Lactamase in Staphylococcus aureus.  ANALYTICAL CHEMISTRY,      [PMID:24844761] [10.1021/ac501288e]

5. Junfeng Liu, Liying Zhang, Haodi Ma, Haoyang Sun, Shu-ai Ge, Jieyi Liu, Shengdi Fan, Chunshan Quan.  (2024)  Quaternary ammonium chitosan-functionalized mesoporous silica nanoparticles: A promising targeted drug delivery system for the treatment of intracellular MRSA infection.  CARBOHYDRATE POLYMERS,      [PMID:39843087] [10.1016/j.carbpol.2024.123184]