Poly-D-lysine hydrobromide - Mn~84000 Da by NMR (equivalent to Mw 150-300 kDa by viscosity ) , CAS No.27964-99-4

CAS: 27964-99-4 Cat. No.: P477809 Formula: D-Lys-(D-Lys)n-D-Lys·xHBr EC Number: 959-037-4 PubChem CID: 87493163

GRADE & PURITY Mn~84000 Da by NMR (equivalent to Mw 150-300 kDa by viscosity )

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview

Poly-D-lysine hydrobromide is a positively charged synthetic polyamino acid having one HBr per lysine unit. It is a crystalline solid soluble in water. Poly-D-lysine hydrobromide is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This polycation is resistant to enzymatic degradation and has been used to culture a wide variety of cell types.

Poly-D-lysine hydrobromide with a molecular weight greater than 30,000 Da is recommended for cell culture applications. The poly-D-lysine hydrobromide (MW=52,000 Da) is easier to use because it is less viscous in solution, however the poly-D-lysine hydrobromide with an higher molecular weight (MW=84,000 Da) has more attachement sites per polymer chain available to the cells. Both poly-D-lysine hydrobromide and poly-L-lysine hydrobromide can be used as a coating substrate, however certain cells digest poly-L-lysine hydrobromide. In this case, poly-D-lysine, as the attachment factor, is necessary to prevent cell disruption by excessive uptake of L-lysine.

Our poly-D-lysine hydrobromide has been purified by dialysis, sterile-filtered on 0.2um, lyophilized, and stored under Ar.

Specifications

Specifications & Purity

Mn~84000 Da by NMR (equivalent to Mw 150-300 kDa by viscosity )

Biochemical and Physiological Mechanisms

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the c

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Pubchem Sid: 504772518
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504772518
• Canonical Smiles: C(CCN)CC(C(=O)O)N.Br
• IUPAC Name: (2R)-2,6-diaminohexanoic acid;hydrobromide
• InChIKey: MEXAGTSTSPYCEP-NUBCRITNSA-N
• INCHI: 1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H/t5-;/m1./s1
• Isomeric SMILES: C(CCN)C[C@H](C(=O)O)N.Br
• PubChem CID: 87493163

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Alpha amino acids
              • Level8 D-alpha-amino acids

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives - Alpha amino acids
• Direct Parent: D-alpha-amino acids
• Alternative Parents: Medium-chain fatty acids Amino fatty acids Amino acids Monocarboxylic acids and derivatives Carboxylic acids Organic oxides Monoalkylamines Hydrocarbon derivatives Hydrobromides Carbonyl compounds
• Molecular Framework: Aliphatic acyclic compounds
• Substituents: D-alpha-amino acid - Medium-chain fatty acid - Amino fatty acid - Fatty acid - Fatty acyl - Amino acid - Carboxylic acid - Monocarboxylic acid or derivatives - Amine - Hydrobromide - Hydrocarbon derivative - Primary amine - Organooxygen compound - Organonitrogen compound - Organic oxide - Primary aliphatic amine - Organic oxygen compound - Carbonyl group - Organic nitrogen compound - Aliphatic acyclic compound
• Description: This compound belongs to the class of organic compounds known as d-alpha-amino acids. These are alpha amino acids which have the D-configuration of the alpha-carbon atom.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: H2O: soluble 50 mg/mL, clear, colorless
• Sensitivity: light sensitive
• x_flash_point_drop_fahrenheit: Not applicable
• x_flash_point_drop_centigrade: Not applicable

References

1. Hai-Mu Ye, Shu-Fang Yao.  (2017)  Supernucleating Role of Poly(ω-pentadecalactone) during the Crystallization of Poly(ε-caprolactone) Composites.  INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH,      [PMID:] [10.1021/acs.iecr.7b03322]