RM 137-15 , CAS No.R1440118

CAS: R1440118 Cat. No.: R1440118 Formula: C₉₃H₁₅₃N₃O₈ Molecular Weight: 1441.23

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

RM 137-15 is an ionizable lipid. The ability of ionizable lipids to form unstable non-bilayer structures at acidic pH is key for endosomal escape and cytosolic delivery of RNA. RM 137-15 can be used in the preparation of liposomes.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

Molecular Weight

1441.23

Application Protocols

No tested applications, validated assay formats, or recommended dilutions are provided for RM 137-15 in this listing. Follow your internal SOPs and refer to the lot-specific SDS/CoA.

General handling suggestions for assay preparation (informational, not product-specific instructions)

• Stock preparation: If supplied as a solid, prepare a concentrated stock (e.g., 10–50 mM) in anhydrous DMSO. Sonicate and gently warm if needed. Record exact concentration gravimetrically or by qNMR/UV where applicable.
• Aliquoting: Dispense single-use aliquots into inert, low-bind tubes or 96/384-well source plates to avoid freeze–thaw cycles.
• Dilution: Use intermediate DMSO dilutions to achieve final assay concentrations while keeping DMSO at ≤0.5–1% v/v in assay wells, unless your assay tolerates higher.
• Plate handling: Use acoustics or pin tools for low-volume transfers when feasible. Verify absence of precipitation or edge effects by visual inspection and by monitoring control wells.
• Documentation: Record lot number (and SKU R1440118), solution age, and storage history; these metadata are critical for reproducibility.

Note: These are general good-practice considerations and are not a substitute for item-specific, validated protocols.

Biological Roles

No biological target, pathway engagement, or mechanism of action is specified for RM 137-15 in this catalog record. Accordingly, no compound-specific biological roles can be assigned.

General considerations for screening compounds (informational)

• Functional ambiguity: Until the structure and prior annotations (if any) are known, treat the molecule as a chemically defined but biologically uncharacterized entity. Any observed activity should be validated with orthogonal assays and counter-screens to exclude artifacts (fluorescence interference, redox cycling, aggregation, membrane disruption).
• ADME surrogates: If a structure becomes available, in silico predictions (cLogP, TPSA, pKa) can help anticipate permeability and solubility. Experimental microsomal stability, plasma protein binding, and permeability assays (PAMPA/MDCK) provide a preliminary developability profile but are beyond the scope of this listing.
• Off-target risks: Pan-assay interference (PAINS) motifs, luciferase inhibitors, and colloidal aggregators are prevalent in diverse libraries. Use detergent-containing buffers (0.01–0.05% Tween-20) and dynamic light scattering where appropriate to identify aggregation.

Research-use limitation

• For research use only; not for human or veterinary use, diagnostic procedures, or clinical applications. Any biological role determination must come from user-generated data and/or structure-enabled literature searches.

Buffer Applications

Buffer systems are not typically defined for an unspecified small-molecule library member. No item-specific buffer recommendations are provided.

General assay buffer guidance (informational)

• Preparation: If dissolving a DMSO stock into aqueous buffer for biochemical assays, maintain final DMSO at ≤0.5–1% v/v unless validated otherwise for the specific target/enzyme/cell line.
• Common buffers: HEPES, Tris, and phosphate buffers are widely used between pH 6.5–8.0. Select buffer species and ionic strength to maintain target stability and avoid interactions with the test compound (e.g., avoid high phosphate with metal-dependent enzymes if chelation is a risk; unknown here).
• Additives: Low levels of non-ionic surfactant (0.01–0.05% Tween-20 or Triton X-100) can mitigate aggregation artifacts. Include BSA (0.1–0.5 mg/mL) where nonspecific adsorption is problematic.
• Filtration and clarity: After dilution, inspect for precipitation or turbidity; if observed, increase organic co-solvent slightly (keeping within assay tolerance) or adjust ionic strength.

Note: Because the structure and ionization state are unknown, no pKa-driven buffer selection can be offered for this item. For definitive guidance, consult the compound’s CoA or structural data if supplied.

Green Alternatives

With no fixed structure or solvent requirement, environmental impact centers on solvent choices for dissolution, handling, and any follow-up chemistry.

Greener solvent considerations (general guidance)

• Prefer water-based systems where feasible, aided by co-solvents at low percentages (≤1% DMSO) or solubilizers (cyclodextrins), provided they do not confound the assay.
• Replace dipolar aprotics where possible: DMF/NMP can be substituted by more benign options (e.g., propylene carbonate, Cyrene) for certain applications, though biocompatibility must be validated.
• Volatile organics: Minimize chlorinated solvents (DCM, chloroform). If necessary, use closed systems and efficient recovery.

Illustrative comparison (literature/general)

• DMSO vs DMF/NMP: DMSO generally offers a better EHS profile and is widely accepted for biological assays; DMF and NMP carry stricter hazard classifications.
• ACN vs MeOH/EtOH: ACN provides low UV background and fast evaporation but has higher toxicity than alcohols; MeOH/EtOH are greener but may disrupt some assays.

Operational practices

• Scale solutions appropriately to reduce waste; prepare only what is needed for each screening run.
• Use microplate-friendly, low-dead-volume reservoirs to minimize discard volumes.
• Implement solvent capture and recycling where infrastructure allows, and segregate halogenated waste streams to improve downstream treatment.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or manufacturing application is specified for RM 137-15. This product is supplied strictly for research use only and is not intended for formulation development for human or veterinary administration.

Context (general, informational)

• Screening libraries often serve discovery-stage research in biochemical or cell-based assays and may inform structure–activity relationships (SAR). Transition to preformulation requires detailed knowledge of identity, purity profile, polymorphism, and stability—none of which are provided here.
• Regulatory frameworks (USP/EP/JP) and ICH guidelines define acceptable impurity levels, residual solvents, and elemental impurities for clinical materials. Such specifications are not applicable to this research-use-only listing unless explicitly provided on a lot-specific CoA.

If formulation-like studies are performed in vitro

• Ensure all handling remains within non-clinical laboratory settings. Document solvent levels (e.g., DMSO %), pH, osmolality, and excipients used to avoid confounding biological readouts.

Summary

• Pharmaceutical uses: Not applicable to this listing.
• Excipient status: Not specified for this item; refer to CoA/Spec Sheet.
• Any downstream translational use would require comprehensive re-sourcing under GMP with full characterization and regulatory compliance.

Physical Properties

Item-specific physicochemical values are not provided in this catalog record. Where values are absent below, consult the lot-specific CoA or specification sheet.

• Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• Aqueous solubility: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility guidance (general, literature-informed): Library members are commonly supplied as solids or DMSO solutions. If solid, an initial 10–50 mM stock in anhydrous DMSO is typically achievable for many drug-like scaffolds. If precipitation occurs, consider co-solvents (DMF, acetonitrile) or gentle warming (≤40 °C) followed by sterile filtration (0.22 µm) where appropriate for bioassays.

Notes

• Without a defined structure, computed properties (cLogP, TPSA, HBD/HBA) cannot be reported. If you have a structure file, cheminformatics tools (e.g., RDKit, ACD/Labs, ChemAxon) can provide literature/computed values for assay planning. Always treat any computed value as guidance rather than a specification.

Quality and Grades

Item-specific grade and purity are not listed in this record.

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/additives: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret quality for screening compounds (general guidance)

• Library/screening grade: Typically emphasizes verified identity and suitable purity for biochemical or cellular screening. Many groups target ≥95% by HPLC/UPLC; however, thresholds vary by application. Always verify the numeric purity and analytical method on the lot CoA.
• UV profile/LC method: HPLC purity can depend on detection wavelength and gradient. Review chromatograms and peak purity, not just a single number.
• Impurities: Trace synthetic byproducts, solvents, or salts may be present. For sensitive assays, consider additional purification (prep HPLC) or orthogonal QC (qNMR) to confirm assay-ready purity.
• Counter-ions/solvates: If a salt form or solvate is supplied, this impacts molecular weight for dose calculations; verify on the label/CoA.
• Batch traceability: Retain the SKU (R1440118), lot number, and receipt date in ELN records for reproducibility.

When to request more data

• If your application requires metal analysis, water content (KF), residual solvents, or stereochemical purity, request the extended QC package. In the absence of such data, treat these as unspecified for this item.

Reaction and Applications

This item is positioned as a small-molecule library member for research screening and profiling rather than as a general synthetic reagent. No manufacturer application notes are provided.

Typical research uses for library compounds (general guidance)

• Primary/secondary screening: Evaluation in biochemical or cell-based assays to identify hits. Prepare DMSO stocks (e.g., 10 mM) and use serial dilutions to define potency windows while controlling for solvent.
• Mechanistic probes: Once a target hypothesis emerges, structure–activity relationship (SAR) studies can be designed around the supplied scaffold, contingent on knowing the exact structure from the CoA or structure file.
• Analytical reference: Employed as a retention-time marker or for MS tuning if ionization behavior is favorable (to be determined experimentally).

If used in synthesis (contingent on structure disclosure)

• Without functional group information, no specific reactions can be recommended. After obtaining the structure, assess reactive handles (halides, boronates, amines, acids) to plan cross-couplings, amide couplings, or heterocycle elaborations.

Assay considerations

• Track lot identity and solution age; many small molecules are sensitive to hydrolysis, oxidation, or photolysis. Use amber vials, exclude moisture, and minimize freeze–thaw by aliquoting.
• Incorporate counterscreens and orthogonal readouts to mitigate assay interference (fluorescence quenching, aggregation, redox cycling), especially in the absence of structural information.

Reaction Conditions

No structure or functional group data are provided for RM 137-15; therefore, compound-specific reaction conditions cannot be recommended.

General notes (informational)

• If deploying this material as a substrate in exploratory chemistry, first confirm identity and purity by LC–MS and NMR. Conduct small-scale solubility tests across a solvent panel (DMSO, DMF, MeCN, DCM, THF, toluene, MeOH) to determine workable concentrations.
• Stability scouting: Evaluate sensitivity to acid/base, oxidation, and light. Simple tests (e.g., 24 h at pH 2/7/10, room temperature/light vs dark) can inform protective handling.
• Atmosphere and moisture: Until known otherwise, handle under dry conditions; use inert gas (N2/Ar) for air- or moisture-sensitive motifs.
• Temperature: Begin with ambient conditions; escalate cautiously while monitoring by TLC/UPLC to avoid decomposition.

Expected yields and catalysts: Not applicable—insufficient information. Once the structure is known, consult literature precedents for similar scaffolds to select catalysts (Pd, Cu, Ni), bases, and ligands where relevant.

Reminder: For most users this item functions as a screening compound for assays rather than a synthetic building block; the above notes apply only if you intend to explore derivatization with structural information in hand.

Safety and Handling

This product is intended for research use only. No item-specific GHS hazard data are provided in this listing; consult the SDS for your lot as the authoritative source.

GHS and hazard data

• Signal word: Not specified for this item; refer to SDS.
• H-statements: Not specified for this item; refer to SDS.
• Pictograms and classification: Not specified for this item; refer to SDS.

General laboratory precautions for small organic molecules (literature/good practice)

• PPE: Lab coat, safety glasses or splash goggles, and suitable chemical-resistant gloves (e.g., nitrile). Use in a fume hood to avoid inhalation of dusts or solvent vapors.
• Avoid: Ingestion, inhalation of dust/particulates, skin/eye contact. Do not pipette by mouth. Keep away from incompatible oxidizers and strong acids/bases until compatibility is known.
• Handling: Minimize exposure to moisture and light unless stability is established. If supplied in DMSO, avoid repeated freeze–thaw; aliquot on receipt.
• First aid (overview; defer to SDS for details): Eye/skin contact—rinse with water for ≥15 min, remove contaminated clothing; Inhalation—move to fresh air; Ingestion—rinse mouth, seek medical attention. Provide the SDS to responders.
• Spill response: Absorb small spills with inert material (vermiculite, silica). Avoid dust generation. Dispose per institutional and local regulations.
• Fire safety: Assume combustible; use CO2, dry chemical, or foam. Avoid water jets unless appropriate for surrounding materials.

Storage and transport

• Store at −20 °C (per item data). Shipments are made in an ice chest with ice pads to protect from heat excursions.

Solvent Selection

Given the absence of a disclosed structure, solvent selection should follow a rational screening approach suitable for diverse small molecules.

General polarity strategy (literature/general guidance)

• Primary solvent for stock solutions: DMSO (anhydrous) is the default for many screening compounds due to broad solubilizing power and assay compatibility at ≤0.5–1% v/v in final wells.
• Secondary co-solvents (if DMSO alone is insufficient): DMF, NMP, and acetonitrile can enhance solubility; mix with DMSO to maintain assay compatibility. For non-aqueous chemistry, consider dichloromethane, ethyl acetate, THF, toluene, or MeOH/EtOH depending on polarity needs.
• Aqueous compatibility: Use aqueous buffers with surfactants (e.g., 0.01–0.05% Tween-20) or cyclodextrins for poorly soluble hydrophobes; maintain final DMSO ≤1% unless validated.

Practical workflow

• Start at 10–50 mM in DMSO, sonicate briefly, and warm gently (≤40 °C). If undissolved, add ACN (up to 20–50%) or switch to DMF/NMP. Filter through 0.22 µm PVDF/PTFE for clarity in optical assays.
• Check solubility visually and by UV–Vis baseline stability. Avoid precipitation upon dilution by matching solvent percentages in intermediate dilutions.

Comparison (general)

• DMSO vs DMF: DMSO is more biocompatible; DMF offers similar solvency but is less favored in cellular assays.
• ACN vs MeOH/EtOH: ACN has low viscosity and good miscibility; alcohols can perturb some biological readouts—validate empirically.

Storage and Reconstitution

Storage

• Store at −20 °C (per Product Data). Protect from heat excursions; shipments are provided in an ice chest with ice pads. Upon receipt, place immediately in a −20 °C freezer.
• Light/moisture: In the absence of stability data, store in amber vials under dry conditions. Consider a desiccant or inert headspace (N2/Ar) for long-term storage.
• Freeze–thaw: If in solution (e.g., DMSO), aliquot to avoid repeated freeze–thaw cycles that can promote degradation or precipitation.

Reconstitution

• Appearance and solvent: Not specified for this item; refer to CoA/Spec Sheet for supplied form. If supplied as a solid, a practical starting point is to dissolve in anhydrous DMSO to 10–50 mM, then adjust as needed based on observed solubility.
• Technique: Allow vial to equilibrate to room temperature before opening to minimize moisture condensation. Add solvent incrementally with gentle vortexing/sonication. If undissolved, warm mildly (≤40 °C) and consider small fractions of co-solvent (ACN, DMF). Filter through 0.22 µm if clarity is required for optical assays.
• Stability of solutions: In the absence of item-specific data, prepare fresh working dilutions on the day of use. Store concentrated DMSO stocks at −20 °C in amber, tightly sealed vials.

Documentation

• Record preparation date, solvent, concentration, and storage conditions in your ELN. For any deviations or observed instability, annotate and, if needed, request additional stability information for your lot.

Research Use Note

• For research use only.

Structure and Identity

Overview for catalog item RM 137-15 (SKU: R1440118), a small-molecule library member supplied for research screening.

• Chemical name: Not specified for this item; refer to CoA/Spec Sheet.
• CAS: R1440118 (catalog placeholder; not a standard CAS Registry Number).
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (functional groups, rings, stereochemistry): Not specified for this item; refer to CoA/Spec Sheet.

2D structure description

• Without a structure, functional group and scaffold annotations cannot be provided. If you received a structure file with your shipment (SDF/Mol), consult that document for ring systems, heteroatom count, H-bond donors/acceptors, rotatable bonds, and stereocenters (if any). These parameters are commonly used to assess drug-likeness, developability, and to plan solubility/assay conditions.

Identity and traceability

• Category: Small molecules and compound library (小分子和化合物库).
• Recommended verification: LC–MS (exact mass), 1H NMR for identity, and HPLC/UPLC for purity. Compare against the Certificate of Analysis (CoA) provided for your specific lot.
• Note: This is a research-use-only screening compound; no biological target, mechanism, or structural identifiers are supplied in this catalog record.

Synthetic Utility

RM 137-15 is presented as a screening compound, and no structural or functional group information is provided. As such, no compound-specific synthetic transformations can be recommended without the molecular structure.

General guidance if structure becomes available (informational)

• Functional handles: Identify reactive sites (e.g., aryl halides, boronates, carboxylic acids, amines, alcohols) to enable cross-couplings (Suzuki–Miyaura, Buchwald–Hartwig), amide couplings, or heterocycle modifications.
• Protecting groups: Assess the need for protection of nucleophilic/acidic sites prior to multi-step elaboration; choose orthogonal PGs compatible with your planned sequence.
• Stereochemistry: If chiral centers are present, establish enantiopurity by chiral HPLC or SFC and consider asymmetric synthesis routes or resolution strategies.
• Late-stage diversification: Minisci, C–H activation, and photoredox methods can diversify complex scaffolds once innate reactivity is mapped.

Caveats

• Purity specification, salt/solvate form, and polymorph are unknown for this item; these factors can influence reaction outcomes and should be clarified via CoA and preliminary analytics (1H/13C NMR, LC–MS, HPLC). Absent this information, treat the compound as an uncharacterized substrate for synthetic planning purposes.

Target Specificity

No target, pathway, or binding specificity information is supplied for RM 137-15 in this catalog record.

• Antigen/target name: Not specified for this item; refer to CoA/Spec Sheet or associated screening documentation.
• Mechanism/class: Not specified for this item; refer to CoA/Spec Sheet.
• Species selectivity: Not specified for this item; refer to CoA/Spec Sheet.
• Assay-validated activity: Not specified for this item; refer to CoA/Spec Sheet.

Guidance for users (informational)

• If you have supplementary documentation disclosing the structure or target hypothesis, align your assay design accordingly and implement orthogonal readouts to confirm on-target effects (e.g., enzymatic activity vs thermal shift vs binding biophysics).
• Incorporate appropriate negative/positive controls and counterscreens to detect assay interference or off-target phenomena. Without declared specificity, all activity should be considered putative until independently validated.

Frequently Asked Questions

How should this product be stored?

Store at ?20 °C. Freezer storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is R1440118, the molecular formula is C93H153N3O8, and the molecular weight is 1441.23 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.