Slanetz and Bartley Medium - BioReagent

Cat. No.: S1528636

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For isolation and cultivation of Enterococcus and Enterococcus faecalis

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

biological_products

Application Protocols

Below are literature-based, representative workflows. Always adapt to your SOPs and consult the product’s CoA/Spec Sheet for any item-specific instructions.

• Membrane filtration enumeration (water testing; literature):

  1. Filter a measured volume of sample through a sterile 0.45 µm membrane.
  2. Place membrane onto Slanetz and Bartley agar (grid side up), avoiding air bubbles.
  3. Incubate inverted at 35–37 °C for 24–48 h.
  4. Count characteristic colonies (pink to red/maroon). Confirm identity with secondary tests as required by method.

• Spread- or pour-plate method (literature):

  1. Prepare agar per CoA; cool to ~45–50 °C.
  2. For pour plates, mix sample/serial dilutions with molten agar; for spread plates, spread 0.1–0.2 mL onto solidified agar using a sterile spreader.
  3. Incubate at 35–37 °C for 24–48 h and enumerate typical colonies.

• Quality control strains (examples; literature guidance only):

  • Positive control: Enterococcus faecalis (growth with characteristic colony coloration).
  • Negative controls: representative Gram-negatives (e.g., Escherichia coli) showing inhibition.

• Tips:

  • Dry plate surfaces before use to prevent swarming or merged colonies.
  • Record incubation temperature and time precisely; small deviations can affect selectivity and TTC reduction.

Biological Roles

This section addresses biological context relevant to the organisms targeted by Slanetz and Bartley Medium.

• Target organisms (literature):

  • Enterococcus spp. (e.g., E. faecalis, E. faecium) are Gram-positive, catalase-negative lactic acid bacteria inhabiting the gastrointestinal tract of humans and animals and are indicators of fecal contamination in environmental monitoring.

• Basis of selectivity and differentiation (literature):

  • Selectivity: Sodium azide inhibits cytochrome oxidase-containing Gram-negative bacteria, thereby favoring growth of enterococci which tolerate azide better.
  • Differentiation: 2,3,5-Triphenyltetrazolium chloride (TTC) acts as an artificial electron acceptor; metabolically active cells reduce TTC to red formazan, producing red/maroon colonies.
  • Nutrient base: peptides and yeast extract provide amino acids, vitamins, and growth factors, while phosphate buffers stabilize pH for optimal enterococcal growth.

• Incubation characteristics (literature):

  • Typical incubation at 35–37 °C for 24–48 h yields pigmented colonies for enumeration. Some protocols include elevated temperatures (e.g., 41–44 °C) for confirmatory steps depending on standards.

Note: The above biological roles are general literature-based and provided for research-context planning. They are not diagnostic claims.

Buffer Applications

This product is a complete culture medium rather than a defined buffer. It is not typically used as a standalone buffer system.

• Context (literature):

  • Slanetz and Bartley Medium generally contains phosphate salts that provide buffering capacity near neutral pH to support microbial growth and indicator performance.
  • Typical working pH is approximately neutral (around pH 7.0–7.2 after sterilization; literature). Exact pH and buffer composition vary by supplier and lot.

• Practical note:

  • If pH adjustment is required during preparation, use sterile 1 N HCl or 1 N NaOH dropwise and re-check after sterilization, as heat can shift pH. Always follow your lab’s SOP and the product’s CoA/Spec Sheet.

For dedicated buffer preparation or electrophoresis/cell culture buffer systems, choose a defined buffer (e.g., phosphate, Tris) tailored to your application.

Green Alternatives

Greener practice relates to preparation, energy use, and waste minimization rather than substituting the medium itself.

• Opportunities for greener practice:

  • Energy: Consolidate autoclave cycles and use validated minimum hold times to reduce energy consumption while maintaining sterility (follow your sterilizer’s performance qualification).
  • Waste: If the formulation contains sodium azide, collect liquid and solid waste separately and dispose via hazardous waste streams; avoid drain disposal to prevent formation of metal azides in plumbing.
  • Packaging: Use bulk packs where appropriate to reduce packaging waste and shipment frequency; reseal quickly to limit moisture uptake and extend shelf life.
  • Water: Prepare only what you need; unused hydrated medium has limited shelf life—smaller batch sizes reduce discard.

• Comparison (general):

  • Ready-to-use plated media vs dehydrated media
    • Pros (ready-to-use): saves energy/time, no autoclaving; reduces exposure to powdered chemicals.
    • Cons: higher shipping footprint (mass/volume), shorter shelf life, more packaging.
  • Dehydrated media (this product form): efficient storage/shipping; requires autoclaving energy and introduces chemical handling.

Adopt institution-specific green lab guidelines (e.g., My Green Lab) while maintaining required microbiological performance.

Pharmaceutical Uses

No excipient or therapeutic use is intended. Slanetz and Bartley Medium is for research and laboratory use only.

• Relevant non-clinical contexts (literature):

  • Environmental monitoring and water testing labs may use this medium for enumeration of enterococci as indicators of sanitary quality.
  • In pharmaceutical/food QC laboratories, selective media are used for method development, growth-promotion testing (GPT), and environmental isolate characterization. Use must align with internal SOPs and regulatory compendia as applicable.

• Regulatory status:

  • Pharmacopeial monographs do not typically cover this specific medium; users should validate performance per USP/EP/ISO guidance when the medium is incorporated into QC methods.

• Documentation:

  • For research use only (per Product Data). Not for human or animal therapeutic or diagnostic use.

Physical Properties

As a complex dehydrated culture medium, conventional single-compound physical constants (bp, mp, logP) are not applicable.

• Item-specific physical data:

  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight: Not applicable to the whole medium.

• General/literature characteristics (for typical Slanetz & Bartley dehydrated media):

  • Physical form: free-flowing, hygroscopic powder blend (literature).
  • Color (dehydrated): beige to light tan (varies with supplier; literature).
  • Solubility: readily water-soluble; prepare in high-purity water (Type 1/2) with gentle heating and stirring (literature).
  • Typical working pH (at 25 °C): around neutral, commonly near pH 7.0–7.2 after sterilization (literature; not a specification for this item).
  • Density/bulk density: blend-dependent; Not specified for this item.
  • Refractive index, logP, pKa: Not applicable.

Notes:

• Hygroscopic components can clump if exposed to ambient humidity; keep container tightly closed.
• TTC-containing media may exhibit light sensitivity; minimize prolonged light exposure to preserve indicator performance (literature).

Quality and Grades

• Item-specific grade: BioReagent (per Product Data).

  • BioReagent grade indicates suitability for laboratory and research use, including microbiology workflows, with controls on bioburden and contaminant levels appropriate for culture media preparation. Not intended for clinical/diagnostic use.

• What BioReagent means in practice:

  • Consistent nutrient composition and performance characteristics batch-to-batch, enabling reproducible recovery and differentiation of target organisms in research/QC settings.
  • Manufactured with attention to impurities that could inhibit growth (e.g., heavy metals) or confound selectivity.

• Stabilizers/indicators:

  • Presence and level of stabilizers or indicator dyes are formulation-dependent. Not specified for this item; refer to CoA/Spec Sheet.

• Performance considerations:

  • For selective/differential media, QC often includes growth promotion and selectivity checks against target and non-target strains (literature). Users should perform in-house verification consistent with their SOPs and regulatory needs.

• Documentation:

  • For lot-specific certificate of analysis (pH, g/L preparation guidance, growth-promotion results), please consult the CoA/Spec Sheet for this item.

Reaction and Applications

Chemical reaction use is not the principal domain for this product. Instead, its applications center on selective cultivation and enumeration of enterococci.

• Core applications (literature):

  • Selective isolation and enumeration of Enterococcus spp. from water, food, and environmental samples, commonly with membrane filtration or pour-/spread-plate methods.
  • Differentiation via TTC reduction: target colonies often appear pink to red/maroon due to intracellular reduction of TTC to formazan, aiding visualization.
  • Suppression of competing Gram-negative flora through selective agents (e.g., sodium azide), enriching recovery of enterococci.

• Practical tips:

  • Pre-warm plates to room temperature before use to reduce condensation and spreading.
  • For membrane filtration, place membrane grid-side up on the agar surface and roll gently to remove bubbles; incubate inverted to avoid pooling.
  • Validate incubation temperature and time (commonly 35–37 °C, ~24–48 h; literature) for the strains and matrices you test.

• Related workflows:

  • Confirmation tests (e.g., esculin hydrolysis, catalase negativity, Gram stain) can be used post-isolation to verify identity (literature).
  • For high-background matrices, consider pre-enrichment or differential incubation regimens consistent with your SOPs.

Reaction Conditions

Chemical reaction conditions are not applicable. Instead, the relevant parameters are preparation and sterilization conditions for culture media.

• General preparation guidance (literature; follow your CoA/SOP):

  • Reconstitution: Disperse the dehydrated medium in purified water with moderate heating and stirring until fully dissolved. Typical use concentrations are specified by the supplier on the label/CoA (grams per liter). Not specified for this item.
  • pH: Adjust to near neutral as indicated on the CoA. Heat sterilization may shift pH; verify post-autoclave.
  • Sterilization: Autoclave at 121 °C for ~15 minutes (liquid cycle). Do not over-sterilize to avoid nutrient degradation and indicator loss (literature).
  • Indicator addition: If TTC is supplied separately in some protocols, sterile-filter a TTC solution and add aseptically to the base medium cooled to ~45–50 °C before pouring plates (literature practice). Many commercial formulations include TTC pre-blended; follow label instructions.

• Incubation (literature):

  • Typical incubation of inoculated plates at 35–37 °C for 24–48 h. Confirmatory incubation temperatures and times may vary with standards and matrices.

These are literature norms provided for planning; consult the product CoA/Spec Sheet and your institutional SOPs for exact conditions.

Safety and Handling

Follow institutional biosafety and chemical hygiene practices when preparing and using microbiological media.

• GHS and hazard classification (item-specific):

  • Signal Word: Not specified for this item; refer to SDS.
  • H-Statements: Not specified for this item; refer to SDS.
  • GHS Classification/Pictograms: Not specified for this item; refer to SDS.

• General/literature safety considerations for typical Slanetz & Bartley formulations:

  • May contain sodium azide as a selective inhibitor. Sodium azide is acutely toxic; forms explosive heavy metal azides in plumbing. Avoid contact with metals and do not dispose to drains. Collect waste separately according to local regulations.
  • May contain TTC dye; handle to avoid dust inhalation and skin contact. TTC and its reduced formazan are staining agents.
  • Nutrient components are low hazard but can cause irritation as dust.

• PPE and handling:

  • Wear lab coat, nitrile gloves, and safety glasses. Handle powders in a fume hood or powder hood to minimize dust inhalation.
  • Use sterile technique to avoid contamination when preparing plates/broth.

• Storage incompatibilities (literature):

  • Keep dry and tightly capped. Avoid heat, moisture, and strong oxidizers. If present, sodium azide is incompatible with acids and metals.

• First-aid (overview; consult SDS):

  • Inhalation: Move to fresh air; seek medical attention if symptoms persist.
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; seek medical advice if irritation continues.
  • Ingestion: Rinse mouth; obtain medical attention.

Always consult the product-specific SDS for authoritative hazard and response information.

Solvent Selection

This product is a microbiological medium prepared in water; organic solvent selection is not applicable.

• Practical guidance (applicable):

  • Use high-purity water (Type 1 or Type 2) to minimize inhibitory ions and organics.
  • If the formulation includes sodium azide or similar inhibitors, prepare and handle using plastic or azide-compatible glassware; avoid copper/lead drains for waste.
  • For TTC-containing media, protect solutions from strong light during preparation to preserve indicator quality.

• Not typically applicable: polarity indices, dielectric constants, or co-solvent screening. For experimental planning, focus on the preparation conditions and sterilization parameters instead (see Reaction Conditions and Storage & Reconstitution).

Storage and Reconstitution

• Storage (item-specific):

  • Storage Conditions: Room temperature (per Product Data).
  • Shipped In: Normal (per Product Data).
  • Keep container tightly closed in a cool, dry place. Protect from moisture and prolonged light exposure.

• Shelf life and stability:

  • Specific expiry and retest intervals: Not specified for this item; refer to CoA/Spec Sheet.
  • Once opened, promptly recap and, if possible, store with a desiccant. Avoid repeated, prolonged ambient exposure.

• Reconstitution and sterilization (literature; not specifications):

  • Weigh the medium per label (grams per liter; Not specified for this item).
  • Suspend in purified water with gentle heat and stirring until completely dissolved.
  • Adjust pH as indicated on the CoA (typically near neutral; literature) and autoclave at 121 °C for ~15 min. Do not overheat.
  • If TTC or other heat-labile supplements are provided separately, sterile-filter and add to the sterile base cooled to ~45–50 °C.
  • Pour plates in sterile conditions and allow to solidify. Store finished plates at 2–8 °C in the dark, sealed to minimize desiccation (literature practice). Use within the validated in-house shelf life.

• Research Use Note: For research use only (per Product Data).

Structure and Identity

Slanetz and Bartley Medium is a formulated, multi-component microbiological growth medium, not a single defined chemical compound. Accordingly, single-molecule identifiers (CAS, InChIKey, SMILES) do not apply to this product.

• Item-specific identifiers:

  • SKU: S1528636
  • Product Name: Slanetz and Bartley Medium
  • Grade: BioReagent (per Product Data)
  • CAS: Not applicable (complex mixture)
  • InChIKey / SMILES: Not applicable (complex mixture)
  • Molecular formula / Molecular weight: Not applicable to the complete medium; Not specified for this item; refer to CoA/Spec Sheet.

• Structural features (general/literature):

  • Typical medium components (literature): peptones/tryptose, yeast extract, glucose, phosphate buffer salts, selective agent(s) (commonly sodium azide), and a redox indicator dye such as 2,3,5-triphenyltetrazolium chloride (TTC) for colony pigmentation following reduction.
  • Functional roles: nitrogen/carbon sources, buffering species, selective inhibitor(s) suppressing Gram-negative flora, and chromogenic/redox indicator for differential visualization.

• 2D “structure” description (general):

  • The product is a dehydrated blend of low–molecular weight nutrients, peptides, salts, and indicator dye(s). No single 2D structure is representative; each constituent has its own structure.

Synthetic Utility

Not applicable. Slanetz and Bartley Medium is a biological culture medium, not a chemical building block or reagent for synthetic transformations.

• If your workflow involves chemical synthesis alongside microbiological testing, maintain strict segregation of chemical and microbiological work areas to prevent cross-contamination.

For chemistry-focused reagents, see appropriate categories in the catalog.

Target Specificity

This section is primarily for antibodies or affinity reagents. It is not applicable to culture media.

• Applicability note:
  • No antigen/epitope, clone, isotype, or species reactivity applies to this product.
  • For organism selectivity context, see Biological Roles and Reaction & Applications.

Frequently Asked Questions

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.