Sopromidine , CAS No.79313-75-0

CAS: 79313-75-0 Cat. No.: S1449248 Formula: C₁₄H₂₃N₇S Molecular Weight: 321.44

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Sopromidine is a potent and stereoselective isomer of the achiral H2 -agonist Impromidine.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

Molecular Weight

321.44

Application Protocols

No tested application protocols are provided in the Product Data for this SKU. The following are general, non‑item‑specific suggestions for small‑molecule handling in life‑science experiments.

General workflows:

• Stock solution: Prepare a concentrated stock in DMSO (e.g., 10–50 mM) or another suitable solvent. Aliquot and store at −20°C to avoid freeze–thaw.
• Working solutions: Dilute into pre‑warmed assay buffer/media to the desired concentration, ensuring the final organic solvent content is within assay‑tolerated limits (often ≤0.1–0.5% v/v DMSO).
• Controls: Include vehicle controls and, where possible, a known active comparator. Perform serial dilutions to generate full concentration–response curves.
• Stability/adsorption checks: Verify stability over the assay period and minimize adsorption by using low‑bind plastics or glassware.

For any validated protocols specific to Sopromidine (e.g., target‑specific binding or functional assays), consult the primary literature or reach out to Aladdin Technical Support with your lot number for available details.

Biological Roles

Item‑specific biological target information for Sopromidine is not provided in the Product Data. No claims are made here regarding mechanism, potency, or selectivity for any biological target.

General considerations for small‑molecule research tools:

• Such compounds are commonly used to interrogate receptor/ion channel function, enzyme activity, or signaling pathways in cell‑based or biochemical assays. Typical readouts include second‑messenger levels, phosphorylation states, electrophysiology, or binding kinetics.
• In vitro use: Stock solutions are often prepared in DMSO and diluted into assay buffer/media at low final solvent percentages. Adsorptive losses to plastics and serum binding can affect apparent potency; consider low‑bind labware and protein content in media.
• Stability/metabolism: Evaluate stability in the chosen biological matrix (buffer, media, microsomes, S9) to account for hydrolysis, oxidation, or metabolic turnover during the assay timeframe.
• Controls: Include positive/negative controls and, when relevant, orthogonal assays (e.g., biochemical vs. cellular) to confirm on‑target effects versus assay artifacts.

For definitive target specificity, affinity, and kinetic parameters (if available for this compound), consult peer‑reviewed literature and the lot‑specific documentation. This product is for research use only and is not intended for diagnostic or therapeutic applications.

Buffer Applications

This product is a small‑molecule life‑science research tool and is not a buffering agent. Therefore, classical buffer system guidance (pKa, pH range, buffer recipes) is not typically applicable.

Practical notes (general):

• Prepare working solutions by diluting a concentrated stock (often in DMSO or ethanol) into the assay buffer of choice (e.g., HEPES, PBS, Tris) ensuring the final organic solvent fraction remains within the assay‑tolerated limit.
• Verify compound stability at the working pH and ionic strength. Some small molecules exhibit pH‑dependent degradation or precipitation; perform a short stability study (e.g., LC–MS at t = 0, 2, 24 h) under assay conditions.
• If the compound contains titratable groups (unknown here), pH adjustment can aid solubility during preparation; return to physiological pH before use in biological assays.

Green Alternatives

While item‑specific solvent or reagent roles are not defined for this product, greener practices can still be applied to its handling and formulation as a research small molecule.

• Solvent choices (general): Prefer water‑based vehicles with solubility enhancers (e.g., cyclodextrins) over high percentages of organic solvents when compatible with the assay. Where organic solvents are needed, ethanol and propylene carbonate can be preferable to DMF/DMAC based on certain green chemistry metrics.
• DMSO management: If DMSO is required for stock solutions, minimize final assay concentration (≤0.1–0.5% v/v) and consolidate shipments to reduce cold‑chain footprint.
• Waste reduction: Prepare minimal‑volume, high‑concentration stocks to limit waste. Use amber vials to extend solution stability and reduce the need for remakes.
• Energy use: Store at −20°C as specified, but avoid unnecessary ultra‑low‑temperature storage. Batch experimental work to reduce repeated freeze–thaw cycles and associated energy demand.

Illustrative comparison (general):

• DMF vs. ethanol for stock preparation: Ethanol is typically less hazardous and has a better environmental profile, though it offers lower solvating power. Choose ethanol if adequate solubility can be achieved.
• Non‑aqueous vehicles vs. aqueous cyclodextrin formulations: Aqueous cyclodextrin solutions often reduce organic solvent usage and may improve stability; however, they can introduce assay interferences—validate experimentally.

Pharmaceutical Uses

No pharmaceutical or clinical use is claimed or implied for this product. It is supplied strictly for research use only.

General context for formulation scientists (non‑clinical, research setting):

• If exploratory in vivo method development is conducted, common vehicles include aqueous buffers with co‑solvents (e.g., PEG400, ethanol, propylene glycol) or solubilizers (e.g., cyclodextrins). Compatibility, osmolality, and pH should be verified.
• For in vitro ADME profiling, solutions may be prepared in DMSO and diluted into assay buffers for permeability, metabolic stability, or plasma protein binding studies.
• Any references to pharmacopeial standards (USP/EP/JP) would be listed on the CoA if applicable; none are provided in the Product Data for this item.

Reminder: This material is not intended for human or veterinary use, and no excipient or therapeutic roles are asserted.

Physical Properties

Item-specific physicochemical data are not provided in the Product Data for this SKU. Do not treat general values as specifications.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point / Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• logP/logD, pKa, refractive index: Not specified for this item; refer to CoA/Spec Sheet.

General considerations for small‑molecule life‑science tools (literature/general guidance):

• Many bioactive small molecules are prepared as concentrated stock solutions (e.g., 10–50 mM) in DMSO due to typical hydrophobicity; final assay DMSO is commonly kept ≤0.1–0.5% v/v to minimize biological effects.
• If a salt form exists, ionization can improve aqueous solubility; confirm the exact form (free base vs. salt) on the CoA.
• For poorly soluble compounds, co‑solvent blends (DMSO:water or ethanol:water), aqueous vehicles with cyclodextrins, or gentle warming/sonication may assist dissolution. Filter sterilization (0.22 µm) is often feasible for clear solutions.

Important: Treat any literature or computed values as context only. Batch‑specific physical properties and analytical data (HPLC purity, residual solvents, water content) are provided on the CoA/Spec Sheet and supersede general guidance.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.

How to interpret quality information for this product line (general guidance):

• For life‑science research chemicals, the CoA typically lists identity confirmation (e.g., 1H NMR/LC–MS), purity by HPLC/UPLC (area %), and may include residual solvents and water by Karl Fischer. These are batch‑specific and define the release specifications.
• If a salt form or solvate is offered, the CoA clarifies counter‑ion content and assay on anhydrous basis, which is important for accurate molar dosing.
• Low‑level impurities can influence bioassay outcomes at high potency; review chromatograms and impurity profiles on the CoA when designing experiments with narrow therapeutic windows or target selectivity studies.
• UV cutoff/fluorescence background: If the compound will be used in optical assays, check the Spec Sheet for UV–Vis spectra or note that “HPLC grade/low UV” labels (when present) indicate minimized background in chromatographic detection.
• Stabilizers: If any stabilizer or formulating excipient is present, it will be disclosed on the Spec Sheet. Absence of such information typically indicates neat material.

For this SKU, rely on the lot‑specific CoA/Spec Sheet for definitive purity, form (free base vs. salt), and analytical methods.

Reaction and Applications

This product is categorized under life sciences and is typically used as a small‑molecule research tool rather than as a synthetic reagent or solvent.

• Synthetic reaction use: Not typically applicable. There are no item‑specific manufacturer application notes describing use in named reactions or as a building block.
• Practical laboratory applications (general for bioactive small molecules):
  • Ligand screening in receptor/enzyme assays, binding studies (radioligand displacement, SPR, MST), or cell‑based functional assays.
  • Method development for analytical detection (LC–MS/MS calibration standards, stability studies) when investigating metabolism or degradation pathways.
  • Structure–activity relationship (SAR) programs may use such reference ligands as internal controls or comparators.
• Handling tips (general): Prepare fresh aliquots of stock solutions to minimize freeze–thaw cycles. Validate concentration by UV–Vis or quantitative NMR where feasible. For long incubations, assess compound stability in the chosen medium (e.g., via time‑course LC–MS) to account for hydrolysis/oxidation.

For reaction chemistry guidance (e.g., cross‑couplings, condensations), refer instead to building blocks and reagents designed for synthesis. Focus sections more relevant to this product include Safety & Handling, Solvent Selection, Biological Roles, and Storage & Reconstitution.

Reaction Conditions

Not typically applicable. This product is positioned as a small‑molecule research tool rather than a reagent for chemical synthesis, and no item‑specific reaction condition guidance is provided.

General laboratory guidance (when preparing solutions):

• Stock preparation: Dissolve the compound in an appropriate solvent (commonly DMSO for hydrophobic molecules) with gentle warming and vortexing. Avoid prolonged heating. Filter if particulates remain.
• Stability checks: Assess solution stability at intended storage temperature and assay temperature/time using LC–MS or HPLC.
• Light/air sensitivity: In the absence of item‑specific data, prepare and store solutions in amber vials under inert atmosphere only if instability is suspected.

For named reactions, catalysts, temperatures, and yields, refer to reagents with defined synthetic roles and published procedures.

Safety and Handling

Hazard communication (item-specific):

• Signal word: Not specified for this item; refer to SDS.
• H‑Statements: Not specified for this item; refer to SDS.
• GHS classification/pictograms: Not specified for this item; refer to SDS.

General laboratory safety (applicable best practices):

• Use in a chemical fume hood and avoid inhalation, ingestion, and skin/eye contact. Wear appropriate PPE: lab coat, safety glasses or splash goggles, and suitable gloves (e.g., nitrile). Remove contaminated clothing and wash exposed skin with soap and water.
• Incompatibilities are substance‑specific; without item data, avoid strong oxidizers/reductants, strong acids/bases, and ignition sources until the SDS confirms compatibility. Segregate from foodstuffs and incompatible reagents.
• First aid (overview; defer to SDS): eye—rinse with water for several minutes; skin—wash with soap/water; inhalation—move to fresh air; ingestion—rinse mouth, seek medical advice. Provide the SDS to medical personnel.
• Spill/accidental release: Avoid dust/aerosol generation. Absorb liquids with inert material; for solids, carefully sweep/collect to a compatible container. Dispose per local regulations.
• Thermal stability: Follow storage guidance (−20°C) to preserve integrity; avoid repeated freeze–thaw where possible.

Authoritative safety information is found in the product SDS for the specific lot; always consult it prior to use.

Solvent Selection

Item-specific solubility and solvent recommendations are not provided in the Product Data. The following are general strategies for small‑molecule life‑science tools; verify suitability experimentally and consult the CoA/Spec Sheet.

• Primary solvent for stocks: DMSO is commonly used to prepare 10–50 mM stocks for hydrophobic ligands; dilute into assay buffer to ≤0.1–0.5% v/v DMSO final.
• Alternative solvents: Ethanol or DMF can be used where compatible. For in vivo method development (non-clinical), co‑solvent systems such as PEG400:saline or Captisol/cyclodextrin solutions may enhance solubility.
• Aqueous formulation aids: Adjust pH if a titratable group is present (acidic species dissolve better at high pH; basic at low pH). Consider (2‑hydroxypropyl)‑β‑cyclodextrin to increase apparent solubility for hydrophobic analytes.
• Filtration: After dissolution, pass through a 0.22 µm PTFE (organic) or PES (aqueous) filter to remove particulates when sterility/clarity is required.
• Avoid precipitation: Add concentrated organic stock to vigorously mixed buffer to minimize local supersaturation; include surfactant (e.g., 0.01% Tween‑80) only if compatible with the biological system.

Comparison (general):

• DMSO vs. ethanol: DMSO offers higher solvating power; ethanol may be preferred for alcohol‑tolerant systems or when limiting DMSO artifacts.
• DMSO vs. DMF: DMF is also strong but is often avoided in biological contexts due to cytotoxicity; DMSO is typically preferred.

Storage and Reconstitution

Item‑specific conditions (from Product Data):

• Storage: Store at −20°C.
• Shipping: Ice chest + ice pads (cold‑chain).

General guidance for reconstitution (not item‑specific; verify experimentally):

• Solvent selection: If the compound is hydrophobic, dissolve in anhydrous DMSO to prepare a concentrated stock (e.g., 10–50 mM). If a salt form is supplied and is water‑soluble, sterile water or buffer may be suitable. Exact solubility is not specified for this item; refer to CoA/Spec Sheet.
• Technique: Allow the vial to equilibrate to room temperature while sealed to avoid condensation. Add solvent incrementally with gentle vortexing/sonication. Avoid excessive heat or basic/acidic conditions unless supported by data.
• Aliquoting: Dispense single‑use aliquots into amber, low‑bind microvials to minimize freeze–thaw and light exposure. Purge headspace with inert gas if oxidative sensitivity is suspected.
• Storage of solutions: For DMSO stocks, store at −20°C or colder in sealed, moisture‑tight vials. For aqueous working solutions, use promptly or store short‑term at 2–8°C; confirm stability by analytical methods.

Always consult the lot‑specific CoA/Spec Sheet and SDS for definitive guidance on solvent compatibility, solution stability, and safe handling. Research use only.

Structure and Identity

• Product name: Sopromidine (SKU: S1449248)
• CAS: 79313-75-0
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Synonyms/Identifiers: Not specified for this item; refer to CoA/Spec Sheet.

Structural features (general guidance):

• Item-specific structural descriptors are not provided in the Product Data. For unambiguous identity confirmation, consult the Certificate of Analysis (CoA), Spec Sheet, or SDS accompanying the lot you receive.
• If structural identifiers become available (SMILES/InChI), they enable quick recognition of functional groups, ring systems, and potential ionization sites, which in turn inform solubility and handling strategies in life-science assays.

2D structure description:

• Not provided for this item. Where applicable, Aladdin provides 2D depictions showing heterocycles, aromatic motifs, and cation/anion-form information. Please refer to the CoA/Spec Sheet for definitive structural representation.

Synthetic Utility

Sopromidine is offered here as a life‑science research chemical and not as a synthetic reagent or building block. No item‑specific functional group information or reactivity profile is provided in the Product Data.

General notes (when handling bioactive small molecules in synthesis workflows):

• If derivatization or bioconjugation is contemplated for probe development, first confirm the presence and accessibility of modifiable sites (e.g., amines, phenols) from definitive structural data (SMILES/InChI). None are provided here; consult the CoA or literature before planning transformations.
• For analytical support, develop stability‑indicating LC–MS methods to monitor integrity during any chemical manipulation.

Given the lack of structural identifiers in the Product Data, no further synthesis‑focused guidance is provided. For building blocks optimized for cross‑couplings, condensations, or protection/deprotection sequences, please see our dedicated organic synthesis categories.

Target Specificity

• Target/Pathway: Not specified for this item; refer to CoA/Spec Sheet and primary literature.
• Species selectivity: Not specified for this item; refer to CoA/Spec Sheet.
• Modality (agonist/antagonist/inhibitor, etc.): Not specified for this item; refer to CoA/Spec Sheet.
• Potency metrics (Ki, IC50, EC50): Not specified for this item; refer to CoA/Spec Sheet.

Notes:

• If using this compound as a reference ligand or probe, confirm the exact form (free base vs. salt), test system, and reported conditions from peer‑reviewed literature. Potency can vary across assay formats (binding vs. functional) and by species isoform.
• Always include appropriate positive and negative controls to ensure observed effects are on‑target.