Sucrose Solution (2.5 M) - BioReagent, for Cell culture, Sterile-filtered, Ready-to-use, 2.5 M in water

Cat. No.: S1527934

GRADE & PURITY Sterile-filtered, BioReagent, Ready-to-use, for Cell culture, 2.5 M in water

Synonyms

Sterile 2.5 M Sucrose stock solution

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Cell Culture

Overview

This product is a high‑concentration aqueous sucrose solution formulated to an accurate molar concentration of 2.5 M.

For density‑gradient centrifugation applications, it serves as a critical supporting reagent for the isolation of subcellular particles and viruses using cell separation medium. Mix the stock cell‑separation medium with this product at a 9:1 volume ratio to prepare an isotonic stock master solution. Further dilute the master solution with 0.25 M sucrose solution to the target density for isopycnic density‑gradient centrifugation and separation of organelles including mitochondria, lysosomes and microsomes, as well as viruses and exosomes. The sucrose‑based system effectively prevents aggregation of subcellular components under salt‑ion conditions and preserves the structural and functional integrity of particles.

In addition, this product can be used as a cryoprotectant or osmotic buffer component to maintain the structural integrity of biological samples under low‑temperature conditions. It is a fundamental reagent for laboratory biochemical experiments and tissue culture.

Instructions for Use

1.Sterile handling: Inspect the bottle seal for integrity before use. Aspirate the required volume with a pipette or sterile graduated cylinder inside a biosafety cabinet. Avoid repeated freeze‑thaw cycles and repeated cross‑contaminating withdrawals.

2.Preparation of isotonic stock master solution (SIM): Mix the stock cell‑separation medium with this product at a 9:1 volume ratio (e.g., 90 mL stock cell‑separation medium + 10 mL 2.5 M sucrose solution). Slowly add this product into the stock cell‑separation medium, and mix thoroughly using a magnetic stirrer or vortex mixer.

3.Dilution to target working solution: Dilute the isotonic stock master solution with 0.25 M sucrose solution to the target working‑solution density according to experimental requirements. Prepare diluted working solutions fresh before use to prevent contamination or density drift upon prolonged storage. Refer to the cell‑separation medium manual for detailed volume‑calculation formulas.

4.In‑situ gradient separation: Directly mix the sample to be separated (subcellular particles or viruses) with the diluted working solution, then perform high‑speed centrifugation. A continuous density gradient forms spontaneously during centrifugation, and target particles migrate to their isopycnic positions.

5.Other applications: When used for osmotic adjustment or cryoprotection, dilute to the target concentration with sterile deionized water, calibrate osmotic pressure, perform strict aseptic manipulation, and prepare fresh for immediate use.

Precautions

1.Tighten the cap after opening to avoid concentration shift caused by water evaporation and microbial contamination.

2.If crystals precipitate after long‑term storage (especially under low‑temperature conditions), warm the bottle gently in lukewarm water and shake thoroughly until crystals are fully dissolved prior to use. Discontinue use immediately if turbidity or visible particulate matter is observed.

3.This product is intended for the isolation of subcellular particles and viruses. For cell samples such as lymphocytes and monocytes, use NaCl‑based systems (1.5 M NaCl Solution).

4.For laboratory research use only. Not for human diagnostic or clinical therapeutic applications.

5.Wear a lab coat and disposable gloves for personal safety and health during operation.

Specifications

Synonyms

Sterile 2.5 M Sucrose stock solution

Specifications & Purity

BioReagent, for Cell culture, Sterile-filtered, Ready-to-use, 2.5 M in water

Stability And Storage

Store at 2-8°C long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Cell culture, Ready-to-use, Sterile-filtered

Product Line

biological_products

Application Protocols

Tested application details, recommended dilutions, and validated use protocols are not provided for this item. Not specified for this item; refer to CoA/Spec Sheet and your laboratory’s SOPs.

General guidance (literature/institutional best practice) should be obtained from peer-reviewed protocols appropriate to your specific application (e.g., sucrose cushions for virus purification, organelle isolation in 0.25–0.32 M sucrose, or continuous gradients for ribosome profiling). Validate any protocol with small-scale pilots before routine use.

Biological Roles

• Literature overview of sucrose biology (context; not specific to this item)

  • In plants: Sucrose is a principal transport carbohydrate, moving photosynthate from source to sink tissues via the phloem. It serves as a carbon and energy reservoir and a signaling molecule affecting gene expression and developmental pathways.
  • In microbes: Many microorganisms catabolize sucrose via invertase or sucrose phosphorylase, yielding glucose and fructose (or glucose-1-phosphate + fructose) for glycolysis and related pathways.
  • In animals: Sucrose is a dietary disaccharide hydrolyzed by sucrase-isomaltase in the small intestine. It is not synthesized by mammals and does not serve as an intracellular metabolite.
  • Nonreducing behavior: Because both anomeric centers are involved in the glycosidic bond, sucrose is nonreducing and less prone to Maillard reactions than reducing sugars—beneficial for stabilizing some biomolecules in storage.

• Roles in laboratory biology

  • Osmotic and density modulation: Provides a controllable, nonionic means to adjust extracellular osmolality and create density media for centrifugation of organelles, membranes, and viral particles.
  • Protein and membrane stabilization: As a kosmotropic polyol, sucrose can help preserve protein tertiary structure and membrane integrity during stress (cooling, freezing, drying), though optimal concentrations are system-dependent.

• Note

  • These are literature-based roles. Suitability for a given cell type or assay should be empirically validated.

Buffer Applications

• Applicability

  • Sucrose is not a pH buffer. However, it is widely used as a density/tonicity modifier in buffer systems for cell and molecular biology.

• Literature-guided use cases (not item specifications)

  • Density cushions: Commonly 20–60% w/v sucrose in buffered saline or Tris-based buffers to cushion pelleting of organelles or viral particles during ultracentrifugation.
  • Continuous gradients: 5–45% w/v (or ~0.15–1.3 M) prepared by gradient mixers for resolving macromolecular complexes by sedimentation rate or buoyant density.
  • Osmotic stabilization: 0.2–0.8 M sucrose in homogenization buffers to maintain organelle integrity (e.g., mitochondria, lysosomes) during mechanical disruption.
  • Assay compatibility: Nonionic and generally compatible with many enzymatic assays, though high concentrations can affect viscosity, diffusion, and spectroscopic baselines; validate as needed.

• Practical tips

  • Prepare gradients with degassed, filtered buffers to minimize bubble formation; allow gradients to equilibrate to run temperature.
  • Use the supplied 2.5 M stock to make working solutions aseptically; verify osmolality with an osmometer when critical.

• Item-specific notes

  • Exact pH and buffer composition: Not applicable—this product is sucrose in water. Any buffer additives must be introduced by the user under sterile conditions.

Green Alternatives

Sucrose solutions are already considered benign from an environmental and safety standpoint relative to many laboratory reagents.

• Green profile (literature/general)

  • Renewable origin: Sucrose is derived from biomass (sugarcane/sugar beet).
  • Low toxicity: Generally recognized as low toxicity; aqueous medium avoids volatile organic solvents.
  • Biodegradability: Readily biodegradable; primary concern is microbial growth in waste streams rather than persistence.

• Alternatives and trade-offs (literature)

  | Option | Advantages | Considerations | |---|---|---| | Trehalose solution | Superior protein and membrane stabilization in some systems; nonreducing | Typically higher cost; different osmotic/solubility profile | | Glycerol solution | Effective cryoprotectant; miscible with water; adjusts viscosity | Can interfere with some assays; higher viscosity at comparable osmolarity | | Mannitol/sorbitol | Nonionic osmolytes; crystalline solids readily prepared | Reducing sugars (sorbitol) can participate in reactions; mannitol’s solubility limits max osmolarity | | Iohexol/iodixanol gradients | Low viscosity, isosmotic gradients | Expensive; may impact downstream analyses |

• Takeaway

  • For many cell biology density and osmolality tasks, sucrose is a balanced, low-impact choice. Select alternatives based on specific stability needs, assay compatibility, viscosity constraints, and cost.

Pharmaceutical Uses

This product is labeled for research use only. The following information reflects general excipient roles of sucrose solutions in pharmaceutical science (literature) and does not constitute suitability for clinical use.

• Excipient roles (literature/general)

  • Tonicity agent: Adjustment of osmolality in parenteral and ophthalmic formulations when compatible.
  • Stabilizer/cryoprotectant: Helps protect proteins, peptides, and vaccines during freeze–thaw, lyophilization, and storage by preferential exclusion and glass formation.
  • Bulking agent: Provides cake structure in lyophilized products.
  • Nonreducing sugar advantages: Reduced propensity for Maillard-type reactions compared to reducing sugars.

• Considerations

  • Endotoxin/bioburden, residual solvents, elemental impurities, and pharmacopeial compliance (USP/EP/JP) are critical for clinical products. These are Not specified for this item; it is not intended for human or veterinary use.
  • Formulation impacts: High concentrations increase viscosity and can alter diffusion, dissolution, and syringeability; assess device compatibility and filterability.

• Takeaway

  • If translational or GMP applications are anticipated, select pharmacopeial-grade materials with documented compendial compliance and full excipient characterization rather than research-grade reagents.

Physical Properties

• Item-specific specifications

  • Appearance, density, refractive index, pH, UV cutoff: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/general properties of sucrose and concentrated sucrose solutions (for context; not specifications for this lot)

  • Solubility: Sucrose is highly water-soluble; concentrated aqueous solutions readily form at ambient temperature (literature).
  • Volatility: Negligible (nonvolatile solute). The solvent is water; solution volatility reflects water evaporation (literature).
  • Acid/base behavior: Sucrose is neutral; no relevant pKa in the physiological range (literature).
  • LogP: Not applicable (ionic/partitioning descriptors are not meaningful for this highly polar polyol; literature).
  • Optical activity: Neat sucrose is dextrorotatory; solution specific rotation depends on concentration and temperature (literature).
  • Viscosity: Increases markedly with concentration; 2.5 M is notably more viscous than water, which can affect pipetting and filtration (literature trend).
  • Refractive index/Brix: Refractive index rises with concentration; Brix correlates with weight percent. At ~2.5 M the solution typically exhibits high Brix and elevated refractive index relative to water (literature trend).
  • Freezing/boiling behavior: Concentrated sucrose depresses freezing point and elevates boiling point relative to pure water (colligative effects; literature). Avoid assuming exact phase-transition temperatures without measurement.

• Practical implications

  • Allow time for temperature equilibration before volumetric measurements; viscosity and density are temperature-dependent.
  • When precise osmolality is critical, verify by osmometer rather than assuming ideal behavior.

Quality and Grades

• Item-specific grade statements (from Product Data)

  • Sterile-filtered
  • BioReagent
  • Ready-to-use
  • For cell culture

• What these mean in practice

  • Sterile-filtered: Solution has been passed through a sterilizing-grade membrane (commonly 0.2 µm in typical practice; exact pore size not specified for this item). This minimizes viable bioburden at time of filling.
  • BioReagent: Indicates suitability for common biochemical workflows. It does not, by itself, specify endotoxin, bioburden, or mycoplasma limits. Those parameters are Not specified for this item; refer to CoA/Spec Sheet if critical to your application.
  • Ready-to-use: No dilution or dissolution is required to obtain 2.5 M sucrose; use directly or dilute aseptically to the desired working concentration.
  • For cell culture: Formulated and packaged to support cell culture workflows. Nonetheless, validate compatibility with your medium, cell type, and osmolality targets.

• Additional quality considerations

  • Appearance, pH, refractive index, and osmolality acceptance criteria: Not specified for this item; refer to CoA/Spec Sheet.
  • Stabilizers/preservatives: Not specified for this item. If your application requires preservative-free or specific additives, confirm by CoA.
  • UV absorbance/LC suitability: Not specified for this item; if using in analytical workflows, pre-validate background absorbance.

Reaction and Applications

This 2.5 M sterile sucrose solution is primarily used in life science workflows rather than synthetic reactions.

• Common laboratory applications (literature/general)

  • Density cushions and gradients for ultracentrifugation (e.g., organelles, viruses, protein complexes). Sucrose’s nonionic, nonreducing nature and tunable density make it a standard medium.
  • Osmotic stabilization for cells, protoplasts, and organelles; modulation of extracellular osmolality without introducing ionic species.
  • Cryo/lyoprotectant component in buffers for protein and enzyme storage, where nonreducing sugars can help stabilize structure during freezing/drying.
  • Mechanical protection: Increased viscosity and density can reduce shear-mediated damage in certain manipulations.

• Practical tips

  • Warm to room temperature and mix gently before use to ensure homogeneity; viscosity can create stratification.
  • For precise osmolality targets, measure with an osmometer after mixing with your medium.
  • Maintain sterility: If the solution is opened repeatedly, consider aliquoting into sterile containers.
  • Avoid autoclaving post-supply; thermal treatment can induce caramelization or hydrolysis, compromising performance.

• Not typically used for

  • Organometallic or low-polarity organic reactions; the aqueous, highly polar nature is incompatible with most water-sensitive chemistries.

Reaction Conditions

This reagent is not intended for chemical synthesis but for biological workflows. Nevertheless, literature-based operational conditions for common sucrose-solution uses are summarized for context (not specifications):

• Density gradients/cushions (ultracentrifugation)

  • Typical bands: 10–60% w/v sucrose in suitable buffer (e.g., TBS, PBS, Tris). Run temperatures 4–20°C depending on sample stability. Rotor speeds often 100,000–200,000 × g for 1–3 h for cushions; 2–18 h for continuous gradients, varying with analyte.

• Organelle isolation buffers

  • Sucrose concentrations often 0.25–0.32 M for isotonic homogenization of mammalian tissues; keep at 0–4°C to preserve integrity. Include appropriate salts and inhibitors per protocol.

• Protein/enzyme stabilization

  • Add 2–20% w/v sucrose to storage buffers as needed; validate activity and assay compatibility. Store cold to limit microbial growth.

• Practical guidance with this item

  • Use aseptic technique throughout. Mix gently to avoid air entrainment. Verify gradient densities with refractometry or densitometry when precision is required.

• Caveats

  • Exact times, speeds, and concentrations are highly system-dependent; consult organism- or complex-specific literature. For regulated work, lock down parameters via internal validation.

Safety and Handling

• GHS and hazard classification

  • For this specific item: Not specified; consult the product SDS for authoritative hazard, pictograms, and H/P statements.
  • General literature note: Sucrose and its aqueous solutions are generally not classified as hazardous; however, sterile solutions for cell culture must be handled aseptically.

• Handling guidance (general best practice)

  • Use appropriate PPE: lab coat, gloves, and eye protection to avoid contamination and splashes.
  • Work in a clean area or biosafety cabinet when maintaining sterility for cell culture applications.
  • Avoid heating/boiling; thermal decomposition (caramelization/Maillard chemistry in presence of amines) can occur and will compromise suitability for biological work.
  • Incompatibilities: Strong oxidizing agents; concentrated acids/bases can hydrolyze sugars.

• Microbiological considerations

  • High sugar content supports microbial growth if contaminated. Keep containers closed, minimize time at room temperature, and use sterile technique.
  • Do not re-sterilize by autoclaving; filter sterilization is preferred when needed (literature best practice). For this item, it is supplied sterile-filtered.

• First aid (general)

  • Skin/eye contact: Rinse with water. Remove contaminated clothing. Seek medical attention if irritation persists.
  • Ingestion/inhalation: Not expected in normal lab use; follow institutional protocols, seek medical advice as appropriate.

• Spill/cleanup

  • Wipe with water; solution is water-soluble. For sterile areas, disinfect after cleanup to mitigate bioburden.

Solvent Selection

This product is not a general-purpose solvent; it is an aqueous sucrose reagent. Solvent selection per se is typically not applicable.

• Practical compatibility notes (literature/general)

  • Medium compatibility: Readily miscible with water-based buffers, salts, and culture media. Avoid mixing with strong oxidizers or highly acidic/basic solutions intended to remain sterile without subsequent sterile filtration.
  • Polarity: Highly polar matrix dominated by water; sucrose increases solution polarity and hydrogen bonding capacity.
  • Viscosity impact: At 2.5 M, viscosity is significantly higher than water; adjust pipetting speeds and filtration pressures accordingly.
  • Filtration: If blending with other components, use sterilizing-grade filtration after mixing to maintain sterility.

• When to choose this reagent

  • When you need a defined, sterile, high-osmolarity additive to adjust medium osmolality or to prepare density cushions/gradients without the time and variability of in-lab dissolution.

• Alternatives

  • For lower osmolality adjustments: Dilute with sterile water or use lower-molarity sucrose or alternative osmolytes (e.g., NaCl, mannitol, trehalose) according to biological requirements (literature).

Storage and Reconstitution

• Item-specific storage and shipping

  • Storage: Store at 2–8°C (from Product Data).
  • Shipping: Shipped on wet ice (from Product Data).
  • Format: Ready-to-use, sterile-filtered 2.5 M aqueous solution; no reconstitution required.

• Handling and stability considerations

  • Aseptic handling: To maintain sterility during multi-use, open in a clean environment or biosafety cabinet and consider aliquoting into sterile containers.
  • Temperature: Allow to reach room temperature before precise volumetric dispensing to minimize viscosity-related pipetting error; return to 2–8°C promptly after use.
  • Freeze/thaw: Not specified for this item; as a general note, repeated freeze–thaw of aqueous sugar solutions can cause concentration drift due to ice fractionation. Store refrigerated rather than frozen unless your validation supports freezing.
  • Preservatives/stabilizers: Not specified for this item. If long-term storage after opening is required, assess bioburden periodically or use sterile single-use aliquots.

• Shelf life and acceptance criteria

  • Expiration dating, appearance, pH, osmolality, refractive index limits: Not specified for this item; refer to the product label and CoA/Spec Sheet.

• Reconstitution

  • Not applicable; the solution is supplied at use concentration. For working dilutions, use sterile water or sterile buffer, and confirm osmolality as needed.

Structure and Identity

This product is a ready-to-use aqueous solution of sucrose at 2.5 M, intended for life science and cell culture workflows.

• Item-specific facts (from Product Data)

  • Name: Sucrose Solution (2.5 M)
  • Format: Sterile-filtered, BioReagent, ready-to-use, for cell culture
  • Concentration: 2.5 mol/L (aqueous)
  • CAS, InChIKey, SMILES: Not specified for this item; refer to CoA/Spec Sheet.

• Literature identity for the solute (sucrose)

  • Synonyms: Saccharose, β-D-Fructofuranosyl α-D-glucopyranoside (literature)
  • Molecular formula: C12H22O11 (literature)
  • Molecular weight (neutral sucrose): 342.30 g/mol (literature)
  • 2D structural description: A nonreducing disaccharide composed of a glucose unit (α-D-glucopyranose) linked to a fructose unit (β-D-fructofuranose) via an α(1→2)β glycosidic bond. No free anomeric carbon remains, accounting for its nonreducing character (literature).
  • Functional groups: Multiple secondary alcohols, one primary alcohol; acetal/ketal linkages composing the glycosidic bond (literature).
  • Stereochemistry: Defined D-configurations at all carbohydrate stereocenters; fixed α/β configuration at the glycosidic linkage (literature).

• Computed/concentration-derived notes (informational)

  • A 2.5 M solution contains 2.5 mol sucrose per liter of solution; mass of sucrose per liter ≈ 2.5 × 342.30 ≈ 855.8 g/L (calculated).
  • For a non-electrolyte, osmolarity ≈ molarity; thus 2.5 M corresponds to ~2.5 Osm/L, before activity corrections (literature/general calculation).

Synthetic Utility

Although primarily a life science reagent, sucrose has niche roles and relevance in synthesis (literature context).

• Carbohydrate chemistry

  • Protecting-group chemistry on sucrose enables access to selectively functionalized derivatives used as chiral building blocks or linkers in glycoconjugate synthesis.
  • Glycosylation: Sucrose can serve as a starting point to generate activated donors/acceptors after selective protection and activation of hydroxyls.

• Materials and catalysis context

  • Carbon materials: Carbohydrates, including sucrose, are used as carbon precursors for templated carbons under pyrolysis (outside the scope of an aqueous sterile solution but relevant to sucrose as a feedstock).
  • Green media: Aqueous sucrose can modulate microenvironments and sometimes enhance selectivity in biocatalysis.

• Practical note

  • The supplied 2.5 M sterile aqueous solution is not typically employed directly in organic synthesis due to its high water content and viscosity. If using sucrose as a synthetic precursor, solid sucrose or tailored derivatives are generally preferred.

• Item-specific statement

  • No synthetic-grade specifications (e.g., metal content, residual solvents) are provided for this item; Not specified for this item—refer to CoA/Spec Sheet if such data are required.

Target Specificity

Not applicable. This product is a chemical reagent (aqueous sucrose), not a biological targeting agent. There are no antigen/epitope targets, clone designations, isotypes, or species specificities associated with this item.

Frequently Asked Questions

What does sterile-filtered mean?

sterile-filtered indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.