Sudan IV Staining Solution - BioReagent, for microscopy, Biological Stain, 0.1% , CAS No.85-83-6

CAS: 85-83-6 Cat. No.: S774205 Formula: C₂₄H₂₀N₄O Molecular Weight: 380.44 Beilstein Registry Number: 709018 EC Number: 201-635-8

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.1%

Storage

Protected from light,Room temperature

Shipped In

Normal

Overview

Sudan dyes are more soluble in lipids than in solvents. When the dye comes into contact with lipid-containing specimens, a large amount of dye partitions into lipid structures, staining these structures red. Lipid-soluble dyes such as Sudan dyes, Oil Red O and Nile blue are commonly used to visualize lipids.

Sudan IV stains suberized and cutinized cell walls, fats, volatile oils, resins and other substances red or light red, and serves as a fat stain.

Instructions for Use:

1. Fix in 4% neutral formalin for 24 h.

2. Rinse with water for 10 min.

3. Blot moisture from the tissue.

4. Incubate in Sudan IV staining solution for 5–15 min.

5. Differentiate with 70% ethanol. Optimal result: adipose tissue appears orange-yellow to light red while other tissues remain unstained.

6. Rinse and soak in water to remove excess stain.

7. Mount sections and examine under a microscope.

Staining Results:

Fat: Orange-yellow to light red

Precautions:

1. It is recommended to perform a preliminary test with 1–2 samples when using this reagent for the first time.

2. For personal safety, wear protective equipment such as a lab coat and gloves before handling reagents.

Specifications

Specifications & Purity

BioReagent, Biological Stain, for microscopy, 0.1%

Stability And Storage

Store at room temperature for 12 months. Store in the dark.

Storage

Protected from light,Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy

Product Line

biological_products

Names and Identifiers

Canonical Smiles

CC1=CC=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=CC4=CC=CC=C43)O)C

IUPAC Name

1-[[2-methyl-4-[(2-methylphenyl)diazenyl]phenyl]diazenyl]naphthalen-2-ol

InChIKey

RCTGMCJBQGBLKT-UHFFFAOYSA-N

INCHI

1S/C24H20N4O/c1-16-7-3-6-10-21(16)26-25-19-12-13-22(17(2)15-19)27-28-24-20-9-5-4-8-18(20)11-14-23(24)29/h3-15,29H,1-2H3

Isomeric SMILES

CC1=CC=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=CC4=CC=CC=C43)O)C

WGK Germany

3

RTECS

QL5775000

NSC Number

10472

Molecular Weight

380.44

Beilstein

709018

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Azobenzenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Azobenzenes
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Azobenzenes
• Alternative Parents: Naphthols and derivatives Toluenes 1-hydroxy-2-unsubstituted benzenoids Azo compounds Propargyl-type 1,3-dipolar organic compounds Organopnictogen compounds Organooxygen compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic homopolycyclic compounds
• Substituents: Azobenzene - 2-naphthol - Naphthalene - Toluene - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Monocyclic benzene moiety - Azo compound - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Organic oxygen compound - Organopnictogen compound - Hydrocarbon derivative - Organic nitrogen compound - Organooxygen compound - Organonitrogen compound - Aromatic homopolycyclic compound
• Description: This compound belongs to the class of organic compounds known as azobenzenes. These are organonitrogen aromatic compounds that contain a central azo group, where each nitrogen atom is conjugated to a benzene ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Sensitivity: Light Sensitive
• x_melt_point_char: 199°C

Frequently Asked Questions

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, protected from light. The material is light-sensitive — keep it in its original opaque or amber container.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 85-83-6, the molecular formula is C24H20N4O, and the molecular weight is 380.44 g/mol. InChIKey RCTGMCJBQGBLKT-UHFFFAOYSA-N.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.