Thrombomodulin Mouse mAb - Primary antibody, specific to THBD

Cat. No.: Ab131030

GRADE & PURITY ≥90%(SDS-PAGE), See COA

Application

CLIA, Detection Antibody, ELISA

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Conjugation

Unconjugated

Overview

Mouse anti Human Thrombomodulin Antibody, Monoclonal, is a Detection Antibody could be used for CLIA, ELISA.

Specifications

Product Name

Thrombomodulin Mouse mAb - Primary antibody, specific to THBD

Synonyms

AHUS 6 antibody | AHUS6 antibody | BDCA 3 antibody | BDCA3 antibody | CD 141 antibody | CD141 antibody | CD141 antigen antibody | Fetomodulin antibody | Thbd antibody | THPH12 antibody | THRM antibody | Thrombomodulin antibody | TM antibody | TRBM_HUMAN a

Specifications & Purity

≥90%(SDS-PAGE), See COA

Biochemical and Physiological Mechanisms

Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activ

Host species

Mouse

Specificity

THBD

Immunogen

Recombinant thrombomodulin protein.

Conjugation

Unconjugated

SDS-PAGE

150 kDa

Purification Method

Antigen affinity chromatography

Matched Pairs

[Ab131028] Thrombomodulin Mouse mAb as Capture antibody in CLIA

Concentration

See COA

Product Line

biological_products

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (24 months). Upon receipt, it is recommended to aliquot.

Application

Application

Dilution info

CLIA

Use at an assay dependent concentration

ELISA

Use at an assay dependent concentration

Associated Targets(Human)

THBD Tbio Thrombomodulin (0 Activities)

Discover Target: THBD

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Item-specific validated applications, recommended dilutions, incubation times, antigen retrieval conditions, and detection systems are Not specified for this item; refer to CoA/Spec Sheet.

General notes (non-item-specific, no parameter claims):

• Verify antibody compatibility with your assay format (e.g., ELISA, WB, IHC, IF, IP, FC) and sample type before large experiments.
• Perform a small-scale titration to establish working ranges and signal-to-background on your platform.
• Use appropriate positive/negative controls and include isotype controls where relevant.

Defer to the product’s CoA/Spec Sheet for authoritative, item-specific protocols.

Biological Roles

• Antigen overview (literature): Thrombomodulin (also known as CD141, THBD) is an endothelial cell surface glycoprotein that serves as a high-affinity cofactor for thrombin, modulating protease specificity toward activation of protein C and thrombin-activatable fibrinolysis inhibitor (TAFI). It plays roles in hemostasis regulation, limiting coagulation on intact endothelium.
• Localization (literature): Predominantly expressed on vascular endothelium; also reported on certain hematopoietic and stromal cell types. Exists as a transmembrane protein with an extracellular lectin-like domain, EGF-like repeats, a serine/threonine-rich region, a transmembrane helix, and a short cytoplasmic tail.
• Functional implications (literature):
  • By forming a thrombin–thrombomodulin complex, thrombin’s procoagulant activities are reduced while anticoagulant pathways are engaged via protein C activation.
  • The lectin-like domain has been implicated in modulating inflammatory signaling and cell–cell interactions in vitro.
• Experimental utility (general): Antibodies to thrombomodulin are used to detect endothelial cells, to study coagulation pathway regulation, and to examine endothelial activation states in cell and tissue models.

Note: The above biological description reflects general literature knowledge about the thrombomodulin antigen and does not imply any item-specific validation, performance characteristics, or clinical use.

Buffer Applications

• Working buffers (general, literature):
  • PBS or TBS at pH ~7.2–7.6 are common for antibody dilution. Include 0.05–0.1% Tween-20 for wash steps in ELISA/WB to reduce nonspecific binding.
  • Blocking agents: 1–5% BSA, nonfat dry milk, or serum (species-appropriate) depending on assay to suppress background.
  • Stabilizers for storage/handling: 0.1–1% BSA and/or 5–50% glycerol can enhance stability for aliquots; item-specific formulation Not specified for this item.
• Sample preparation considerations:
  • Avoid chelators/detergents that disrupt antigen–antibody interactions unless assay demands it. Maintain isotonic conditions for cell-based assays.
• Compatibility notes:
  • Sodium azide (if present; Not specified for this item) inhibits horseradish peroxidase; avoid in HRP-based detection steps.
• Recipes (no item-specific claims): Prepare buffers with ultrapure water, filter (0.22 µm) for sterility, and pre-equilibrate to assay temperature.

For this item, exact recommended buffers and dilutions are Not specified; refer to CoA/Spec Sheet and adapt to platform-specific best practices.

Green Alternatives

Green chemistry considerations are limited for aqueous antibody reagents but sustainability can be improved through handling and procurement choices.

• Minimizing waste:
  • Prepare only required working volumes to reduce expired waste.
  • Use reusable secondary containment and avoid single-use plastics where sterility permits.
• Solvent/environmental profile:
  • Antibody formulations are typically aqueous and avoid hazardous organic solvents; maintain this by refraining from adding volatile organics.
• Preservatives and disposal:
  • If sodium azide or other biocides are present (Not specified for this item), segregate waste and avoid copper/lead plumbing. Follow local regulations.
• Storage efficiency:
  • Consolidate shipments (this item ships on wet ice per Product Data) and coordinate deliveries to minimize cold-chain emissions.
• Alternative formats:
  • Concentrated stocks or lyophilized formats can lower packaging and shipping burdens if available; format for this item is Not specified.

Item-specific greener alternatives data are not applicable; consult institutional sustainability guidelines for best practices.

Pharmaceutical Uses

This material is designated “For research use only” and is not intended for human or veterinary use, diagnostic procedures, or as an active pharmaceutical ingredient.

• Formulation/excipient status: Not specified for this item; refer to CoA/Spec Sheet.
• Typical roles of antibodies in manufacturing research (general):
  • Analytical controls and reagents in bioassays, process development, and characterization of cell lines and biomolecules.
  • Affinity capture in exploratory purification workflows (e.g., immunoaffinity columns) during research-scale bioprocess development.
• Regulatory note: Not produced under cGMP for clinical use unless explicitly stated (not specified here). No pharmacopeial monograph is implied.

For any work related to regulated environments, confirm documentation, traceability, and quality attributes directly from the product’s CoA/Spec Sheet.

Physical Properties

• Appearance/physical state: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration/formulation: Not specified for this item; refer to CoA/Spec Sheet.
• pH, buffer composition, preservatives: Not specified for this item; refer to CoA/Spec Sheet.
• Protein extinction coefficient (literature, typical): ε280 ≈ 1.4 (mg/mL)−1 cm−1 for generic IgG, corresponding to A280 ≈ 1.0 for ~0.71 mg/mL solutions (approximate; sequence-dependent).
• Isoelectric point (literature, typical): pI ~6–9 depending on isotype and sequence/glycosylation.
• Aggregation/oligomeric state: Monomeric IgG (literature, typical), but may form dimers/aggregates if mishandled; item-specific data Not specified for this item; refer to CoA/Spec Sheet.
• Solubility (literature, typical): Highly soluble in aqueous buffers (e.g., PBS, TBS) at neutral pH; sensitive to extreme pH, high ionic strength, and repeated freeze–thaw.
• Stability (general): Sensitive to proteolysis, surface adsorption, and thermal stress. Avoid vigorous vortexing and foaming. Item-specific shelf-life Not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Grade/purity: Not specified for this item; refer to CoA/Spec Sheet.
• Research-use only: Labeled “For research use only” — not intended for diagnostic, therapeutic, or clinical applications.
• Typical QC for research antibodies (general, literature):
  • Identity and integrity: SDS-PAGE under reducing/non-reducing conditions; size-exclusion chromatography for aggregate profiling.
  • Binding: ELISA against immobilized antigen; titration curves to gauge apparent affinity/potency.
  • Host contamination/endotoxin: Host cell protein/DNA checks; endotoxin quantification (e.g., LAL) when relevant to cell work.
  • Sterility/bioburden: Filtration (0.2 µm) and/or sterility tests for liquid formulations.
• Isotype, clone, and epitope mapping: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/preservatives (e.g., BSA, trehalose, glycerol, sodium azide): Not specified for this item; refer to CoA/Spec Sheet.
• Practical note: Verify species reactivity and validated applications on the item’s CoA/Spec Sheet before experimental use.

Reaction and Applications

This product is a monoclonal antibody; it is not a chemical reagent for stoichiometric or catalytic organic reactions. Traditional “reaction chemistry” content (e.g., Grignard, cross-coupling) does not apply.

• Relevant laboratory applications (general context; not item-specific):
  • Immunoassays such as ELISA, Western blotting, immunofluorescence/immunocytochemistry, immunohistochemistry, immunoprecipitation, and flow cytometry when properly validated.
  • Antigen capture/purification when immobilized on solid supports (e.g., Protein A/G-coupled resin workflows).
• For this item: Validated applications and tested dilutions are Not specified for this item; refer to CoA/Spec Sheet. Prior to use, confirm compatibility with your assay and sample type.

Reaction Conditions

Not applicable to chemical reactions. As an antibody reagent, performance depends on immunoassay conditions rather than stoichiometric or catalytic reaction parameters. For this item, validated applications, working dilutions, incubation times, and temperatures are Not specified; refer to CoA/Spec Sheet. General optimization typically includes titration of antibody concentration, adjustment of blocking/washing stringency, and verification of antigen retrieval (for IHC) under assay-specific conditions.

Safety and Handling

• GHS classification, signal word, pictograms, H-statements: Not specified for this item; refer to SDS.
• General safety (proteins/antibodies):
  • Handle using standard laboratory PPE: lab coat, gloves, and safety glasses.
  • Avoid ingestion, inhalation of aerosols, and contact with skin/eyes. Use biological safety cabinet if handling large volumes or generating aerosols.
  • If product contains sodium azide or other preservatives (not specified here), prevent contact with acids and avoid disposal to copper/lead plumbing; consult SDS.
• First aid (general guidance; defer to SDS):
  • Skin/eye contact: Rinse with water for several minutes. Remove contaminated clothing.
  • Inhalation: Move to fresh air.
  • Ingestion: Rinse mouth; seek medical attention according to institutional policy.
• Incompatibilities (general): Strong acids/bases, oxidizers, and elevated temperatures can denature proteins. Avoid repeated freeze–thaw cycles to limit aggregation.
• Waste: Follow institutional biosafety and chemical hygiene plans; consult SDS for preservative-specific disposal guidance.
• Always consult the product-specific SDS for authoritative safety information.

Solvent Selection

This product is an aqueous protein reagent rather than an organic-soluble compound; solvent selection focuses on buffer systems rather than organic solvents.

• Buffer compatibility (general, literature):
  • Common working buffers: PBS (pH 7.2–7.4), TBS (pH 7.4–7.6), and assay-specific buffers containing 0.05–0.1% nonionic detergents (e.g., Tween-20) to reduce nonspecific binding.
  • Protein stabilizers: 0.1–1% BSA, 5–10% glycerol, and sugars (trehalose/sucrose) can enhance stability; item-specific formulation Not specified for this item; refer to CoA/Spec Sheet.
• Organic solvents: Not recommended; organic content (e.g., >10–20% methanol, acetonitrile, DMSO) can denature antibodies.
• pH and ionic strength: Maintain near-neutral pH (6.8–7.6) for most IgGs; high salt can mitigate nonspecific interactions in assays but may affect binding.
• Detergents and additives: Minimal, non-denaturing concentrations; avoid anionic detergents like SDS except for analytical denaturation.
• Storage buffer selection: Use the supplied formulation whenever possible; for custom buffers, perform small-scale compatibility checks.
• For this item: Specific buffer composition and recommended diluents are Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

• Storage conditions (item-specific): Store at 2–8°C (per Product Data). Shipped on wet ice.
• Freeze–thaw: Unless the CoA/Spec Sheet explicitly allows freezing, keep at 2–8°C and avoid freeze–thaw cycles to minimize aggregation and loss of activity.
• Light and agitation: Protect from prolonged exposure to light and avoid vigorous mixing/foaming.
• Aliquoting: If permitted by the formulation, prepare working aliquots to reduce handling; use low-bind tubes.
• Reconstitution: Not specified for this item; refer to CoA/Spec Sheet. If provided lyophilized (not specified), reconstitute gently with recommended buffer, allow to equilibrate on ice, and mix by slow inversion.
• Storage buffer/composition, stabilizers, and preservative: Not specified for this item; refer to CoA/Spec Sheet.
• Shelf life/expiry: Not specified for this item; refer to CoA/Spec Sheet.

Always follow the instructions on the vial label and the product-specific CoA/Spec Sheet for definitive handling guidance.

Structure and Identity

• Item type: monoclonal antibody (mAb) specific for thrombomodulin; host: mouse (per product name).
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES/InChI/InChIKey: Not applicable to proteins; not provided for this item.
• Structural features (general, literature):
  • Typical IgG-class mAbs are Y-shaped glycoproteins comprising two heavy chains (~50 kDa each) and two light chains (~25 kDa each) linked by disulfide bonds, with variable domains forming the antigen-binding sites (paratopes) and constant regions (Fc) mediating effector functions.
  • Monoclonal antibodies are clonal, targeting a single epitope on the antigen.
• 2D/primary structure description (general, literature):
  • Linear amino acid sequences folded into immunoglobulin domains (β-sandwich). Variable regions (VH/VL) confer specificity; constant domains (CH1–CH3, CL) define isotype.
• Clone, isotype, epitope, species reactivity: Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

Not applicable. This product is a monoclonal antibody reagent and is not employed as a chemical building block or substrate in synthetic organic chemistry. For practical use, refer instead to sections on Buffer Applications, Application Protocols, and Storage & Reconstitution.

Target Specificity

• Target antigen: Thrombomodulin (per product name).
• Clone, isotype, epitope, and species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Cross-reactivity and blocking/neutralization data: Not specified for this item; refer to CoA/Spec Sheet.
• Positive control tissues/cell lines: Not specified for this item; refer to CoA/Spec Sheet.

Only item-specific data are reported in this section; please consult the product documentation for definitive specificity information.