Tissue Single-cell Enzymatic Digestion Stop Solution - BioReagent

Cat. No.: T1508753

GRADE & PURITY BioReagent

Synonyms

温和终止液 | Dissociation Stop Solution | Digestion Stop Solution | Dissociation Stop Buffer | Enzyme Inhibitor Buffer | Single-Cell Digestion Termination Solution | Stop Buffer for Tissue Dissociation | Digestion Termination Solution

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

Cell Culture, Cell Separation

Overview

This product is used to terminate enzymatic digestion reactions of tissues or cells, rapidly neutralize enzyme activity, and protect cell integrity. It is widely used in the preparation of single‑cell suspensions.It can stop enzymatic digestion of various animal organs (including brain, liver, spleen, kidney, lung, tumor tissue, etc.) mediated by tissue single‑cell dissociation enzymes, thereby protecting tissue cells from damage caused by excessive enzymatic digestion.This greatly improves the success rate and data quality of downstream experiments such as flow cytometry, single‑cell sequencing, and cell culture.

Protocol

1. After completion of enzymatic digestion, add an equal volume or 2× volume of Stop Solution to the digestion system immediately (adjust the volume according to enzyme concentration and reaction conditions).
2. Mix gently and incubate at room temperature for 1–2 minutes to fully terminate enzyme activity.
3. Centrifuge at 300–400 ×g for 5 minutes to collect cells; discard the supernatant.
4. Resuspend the cell pellet in pre‑chilled PBS or medium for downstream experiments including flow cytometry, single‑cell sequencing, and cell culture.

Notes

1. Mix the Stop Solution thoroughly before use. If precipitation occurs, centrifuge and use the supernatant.
2. Enzymatic digestion conditions vary among tissue types. Pre‑experiments are recommended to optimize the volume of Stop Solution and reaction time.
3. This product is sterile. Use aseptic technique to avoid contamination.
4. For research use only.

Specifications

Synonyms

温和终止液 | Dissociation Stop Solution | Digestion Stop Solution | Dissociation Stop Buffer | Enzyme Inhibitor Buffer | Single-Cell Digestion Termination Solution | Stop Buffer for Tissue Dissociation | Digestion Termination Solution

Specifications & Purity

BioReagent

Stability And Storage

Store at 2-8℃ long term (12 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent