TNF alpha Mouse mAb - Primary antibody, specific to Human TNF alpha

Cat. No.: Ab215301

GRADE & PURITY Animal Free, Carrier Free, Validated, ExactAb™, Azide Free, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Detection Antibody, ELISA

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

C08/3E2

Conjugation

Unconjugated

Overview

Mouse anti Human TNF alpha Antibody, clone C08/3E2, could be used for a Detection Antibody in sandwich ELISAs

Specifications

Product Name

TNF alpha Mouse mAb - Primary antibody, specific to Human TNF alpha

Synonyms

APC1 antibody | APC1 protein antibody | Cachectin antibody | DIF antibody | Differentiation inducing factor antibody | Macrophage cytotoxic factor antibody | Tnf antibody | TNF superfamily member 2 antibody | TNF superfamily, member 2 antibody | TNF, macr

Specifications & Purity

Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Biochemical and Physiological Mechanisms

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i

Host species

Mouse

Specificity

Human TNF alpha

Immunogen

Recombinant protein corresponding to Human TNF alpha

Conjugation

Unconjugated

Grade

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Matched Pairs

[Ab156547] TNF alpha Mouse mAb, Capture Antibody

Concentration

1.0 mg/mL

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Images

Sandwich ELISA - TNF alpha Antibody Pair (Ab156547 & Ab215301)

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha Mouse mAb (Ab156547)

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

Application

• Application: Dilution info
• ELISA:

  1.0 µg/mL as a Detection antibody in ELISA

Associated Targets(Human)

TNF Tclin Tumor necrosis factor (0 Activities)

Discover Target: TNF

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)