UBR7 Mouse mAb - Primary antibody, specific to UBR7, Mouse IgG2a

Cat. No.: Ab133358

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2a

Clone Number

K49039_15G1

Conjugation

Unconjugated

Overview

Mouse anti Human UBR7 Antibody, Monoclonal (K49039_15G1), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/1000
IP: 1/100
Protein Relevance:
UBR7 is a putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.

Specifications

Product Name

UBR7 Mouse mAb - Primary antibody, specific to UBR7, Mouse IgG2a

Synonyms

5730410I19Rik antibody | AA589405 antibody | AW557761 antibody | C14orf130 antibody | Chromosome 14 open reading frame 130 antibody | EC 6.3.2.-antibody | FLJ10483 antibody | MGC94098 antibody | MGC9518 antibody | N recognin 7antibody | Putative E3 ubiqui

Specifications & Purity

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Host species

Mouse

Specificity

UBR7

Immunogen

Recombinant protein of human UBR7 (AA 141-420).

Positive Control

WB: HeLa, HEK293, MCF7 and Jurkat cell lysates. IHC: Human testis tissue. IP: T-47D lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Product Line

biological_products

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: UBR7 Mouse mAb (Ab133358)

UBR7 Mouse mAb (Ab133358) - Western Blot
All lanes: UBR7 Mouse mAb (Ab133358) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 48 kDa
Observed band size: 52 kDa
Exposure time: 47.8 s

IP: UBR7 Mouse mAb (Ab133358)

UBR7 Mouse mAb (Ab133358) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of UBR7 Mouse mAb (Ab133358) with T-47D lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. UBR7 Mouse mAb (Ab133358) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: T-47D lysateLane 2: UBR7 immunoprecipitated from T-47D lysate by UBR7 Mouse mAb (Ab133358)
Lane 3: The isotype control antibody instead of UBR7 Mouse mAb (Ab133358) in T-47D lysate.Result: UBR7 Mouse mAb (Ab133358) can immunoprecipitate UBR7. 

IHC: UBR7 Mouse mAb (Ab133358)

UBR7 Mouse mAb (Ab133358) - IHC
IHC analysis of testis tissue by UBR7 Mouse mAb (Ab133358).IHC was performed using sections of the formalin-fixed paraffin-embedded testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with UBR7 Mouse mAb (Ab133358) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in seminiferous ducts are positively stained at the nuclei. 

Application

Application

Dilution info

WB

1/1000

IP

1/100

IHC

1/1000

Associated Targets(Human)

UBR7 Tbio Putative E3 ubiquitin-protein ligase UBR7 (0 Activities)

Discover Target: UBR7

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

No manufacturer-tested applications, recommended dilutions, or positive controls are provided in the Product Data for this item. As such, protocol specifications cannot be given for this product.

General guidance for initiating assay development with monoclonal antibodies (not item-specific; to be optimized by the user)

• Titration: Begin with a broad dilution range (e.g., 0.05–5 µg/mL for blot/IF/IHC primary; 0.1–2 µg per IP) and refine based on signal-to-noise.
• Controls: Include isotype controls, secondary-only controls, and positive/negative biological controls for UBR7 expression.
• Sample prep: Optimize lysis and fixation methods to preserve UBR7 epitopes (choice of RIPA vs. mild detergents; antigen retrieval for IHC as needed).
• Secondary reagents: Match species and isotype; verify cross-adsorption for multi-species samples.

For validated applications and recommended conditions specific to this antibody, refer to the product datasheet/CoA when available.

Biological Roles

UBR7 context (literature; general background, not product-specific):

• UBR7 is a member of the UBR (ubiquitin protein ligase E3 component n-recognin) family associated with the N-end rule pathway. UBR proteins typically recognize destabilizing N-terminal residues on substrates, targeting them for ubiquitination.
• UBR7 contains a UBR-box zinc finger domain; reports suggest roles in ubiquitin signaling and chromatin-associated processes. Some studies have linked UBR7 to regulation of histone-associated functions and protein turnover, though substrate scope and mechanisms may vary by system.
• Cellular localization for UBR-family members can include cytosolic and nuclear compartments; UBR7 distribution may be context-dependent and influenced by cell type and stimuli.

Research applications of anti-UBR7 antibodies (general)

• Detection and quantification of UBR7 protein levels across cell lines and tissues.
• Immunoprecipitation to study UBR7 protein complexes and post-translational regulation.
• Imaging-based localization of UBR7 under different experimental conditions.

Notes

• The exact epitope recognized by this monoclonal antibody, cross-reactivity profile, and species reactivity are not specified for this item; consult the datasheet/CoA for product-specific biological details.

Buffer Applications

Buffering considerations for antibody handling are typically more relevant than solvent selection.

• For use in assays (general guidance; not item-specific)

  • Storage/working buffers: Phosphate-buffered saline (PBS) or Tris-buffered saline (TBS), pH ~7.2–7.6. Include 0.01–0.05% nonionic surfactant (e.g., Tween-20) in wash buffers to reduce nonspecific binding (assay dependent).
  • Blocking agents: Serum proteins (e.g., 1–5% BSA or milk) for immunoassays; choose blockers compatible with detection chemistry.
  • Conjugation buffer compatibility: For NHS-ester labeling, avoid primary amines (e.g., Tris) during coupling; use phosphate or bicarbonate buffers.

• Carrier-free implications

  • Absence of carrier proteins reduces background in sensitive assays but increases adsorption losses. Use low-binding plastics and prepare single-use aliquots.

Item-specific buffer composition or recommended formulations are not provided; refer to the CoA/Spec Sheet for details.

Green Alternatives

Green chemistry comparisons for solvent replacement are not directly applicable to an antibody reagent. Nonetheless, environmentally conscious practices can be applied to antibody handling and workflows.

• Practical sustainability considerations (general)
  • Use minimal single-use plastics; select low-binding, recyclable plastics where feasible.
  • Consolidate cold shipments to reduce dry ice consumption and packaging waste.
  • Employ room-temperature-stable intermediates only when compatible with antibody integrity (not applicable here given storage at −80°C per Product Data).
  • Optimize assay conditions to reduce antibody usage per experiment without compromising data quality.

No greener “alternative solvents” are relevant for this product type.

Pharmaceutical Uses

This product is supplied strictly for research use only (per Product Data); it is not intended for diagnostic, therapeutic, or clinical applications.

• Formulation/excipient context

  • Monoclonal antibodies in pharmaceutical development are typically formulated with stabilizing excipients (e.g., sugars, amino acids, surfactants) and controlled ionic strength/pH. Such excipients are not specified for this item and may be absent due to the carrier-free designation.

• Regulatory status

  • No pharmacopeial monograph or GMP manufacturing claims are provided for this item. For regulated uses, dedicated GMP-grade materials and comprehensive quality documentation would be required.

No medical or clinical claims are made or implied.

Physical Properties

Item-specific physicochemical parameters are not provided.

• Provided for this item

  • Physical state/appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Concentration, formulation, excipients: Not specified for this item; refer to CoA/Spec Sheet.

• Typical monoclonal antibody properties (literature, general guidance; not item-specific)

  • Molecular mass: ~150 kDa for an intact IgG (varies with isotype and glycosylation).
  • Isoelectric point (pI): often in the 6–9 range depending on sequence/glycoform.
  • Solubility: Highly soluble in aqueous buffers (e.g., PBS, HEPES) with stability enhanced by protein stabilizers and appropriate ionic strength.
  • Extinction coefficient (A280): sequence-dependent; commonly approximated by 1.4–1.5 (mg/mL)−1 cm for IgG when sequence is unknown (literature rule-of-thumb).
  • Stability: Sensitive to repeated freeze–thaw, surface adsorption, and agitation-induced aggregation; stability improved by low-temperature storage and gentle handling.

• Refractive index, logP, pKa, BP/MP

  • Not applicable or not meaningful for proteins in aqueous formulations; not specified for this item.

Always rely on the CoA/SDS for definitive, lot-specific physical information.

Quality and Grades

• Item-specific designations (per Product Data)

  • Carrier Free: Supplied without protein stabilizers such as BSA, gelatin, or other carrier proteins. Advantages include compatibility with conjugation, cell-based assays sensitive to exogenous proteins, and mass spectrometry workflows. Tradeoff: increased risk of adsorption loss; handle with low-binding plastics and consider adding carrier only after aliquoting if appropriate for your application.
  • ExactAb™: Trademarked designation by the supplier. Generally implies well-defined, highly consistent monoclonal material. Specific production method (e.g., recombinant vs. hybridoma-derived) and QC criteria are not specified for this item; refer to CoA/Spec Sheet for exact details.
  • Validated: Indicates that functional identity and/or application performance has been verified by the manufacturer. Specific validated applications, species reactivity, and data are not listed in the provided data; consult the product datasheet for validation scope.

• Purity/grade specifics

  • No numerical purity, endotoxin levels, host cell protein/DNA, or bioburden specifications are provided here. For such metrics, see the CoA/Spec Sheet.

• Practical implications

  • Carrier-free format is ideal for user-directed conjugations (enzymatic HRP/AP, fluorophores, biotin) and surface immobilization without carrier interference.
  • For quantitative or regulated workflows, request lot-specific documentation (CoA), including concentration determination method (A280 vs. amino acid analysis), residuals, and sterility profile.

Reaction and Applications

As an antibody reagent, this product is not used as a chemical reactant in small-molecule synthesis. Instead, its value lies in specific antigen recognition.

• Typical laboratory applications for monoclonal antibodies (general; not item-specific/validated)
  • Immunodetection: Western blotting (WB), ELISA, immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and proximity assays.
  • Affinity capture: Pull-down of UBR7 and interacting complexes from cell/tissue lysates.
  • Quantitative assays: Sandwich ELISA or targeted enrichment for proteomics (if paired with compatible secondary/capture reagents).

No manufacturer-tested applications are provided in the Product Data for this specific item; refer to the datasheet for validated use-cases.

Reaction Conditions

Classical reaction-condition guidance (solvent, temperature, catalysts) is not applicable to an antibody reagent. However, conditions for immunoassays and binding interactions can be considered operational parameters.

• General operational parameters for antibody assays (literature; not item-specific)
  • Binding: Many antigen–antibody interactions are conducted at 4–25°C in buffered saline; incubation times vary from 30 min to overnight depending on affinity, matrix, and format.
  • Washes: Use isotonic buffers with mild surfactant to reduce nonspecific binding; adjust stringency as needed.
  • Conjugations: Perform at neutral to mildly basic pH (e.g., pH 7.2–8.5 depending on chemistry). Remove unreacted small molecules by desalting/dialysis.

No validated reaction/assay conditions are specified for this particular item; consult the datasheet for any product-specific recommendations.

Safety and Handling

• GHS/Regulatory

  • Signal word, hazard statements (H-phrases), pictograms, and classification: Not specified for this item; consult the SDS for authoritative information.
  • Intended use: For research use only (per Product Data). Not for diagnostic or therapeutic use.

• General laboratory PPE and practices (good laboratory practice; not item-specific)

  • Wear lab coat, gloves, and eye protection. Avoid inhalation of aerosols and contact with skin/eyes.
  • Handle in a clean area; avoid microbial contamination.

• Handling considerations for antibodies (general)

  • Avoid repeated freeze–thaw cycles to minimize aggregation and activity loss (explicitly stated in Product Data).
  • Minimize foaming and vigorous vortexing; mix gently.
  • Use low-protein-binding plastics or pre-blocked glassware to reduce adsorption losses.

• Storage incompatibilities

  • Do not store with proteases or in buffers containing protease contaminants; avoid metal-catalyzed oxidation (use appropriate buffer systems if needed). Specific incompatibilities are not specified for this item.

• First aid (general guidance; defer to SDS)

  • Skin/eye contact: Rinse with water. Ingestion/inhalation: Seek medical attention if symptoms occur. Provide SDS to responders.

Always consult the product’s SDS for definitive hazard, disposal, and first-aid guidance.

Solvent Selection

This product is an aqueous protein biologic rather than a low–molecular-weight solute; classical “solvent selection” concepts (polarity/logP/solubility in organic solvents) are not typically applicable.

• Guidance relevant to antibodies (general)
  • Use physiological aqueous buffers (e.g., PBS, TBS, HEPES) tailored to the intended application.
  • For conjugation chemistries, ensure buffer components are compatible (e.g., avoid primary amines for NHS-ester reactions; avoid azide for click-ready alkyne/azide pairs or HRP activity).
  • Carrier-free format facilitates buffer exchange by dialysis or spin desalting to meet reaction requirements.

For detailed buffer recommendations, see Buffer Applications; for storage considerations, see Storage & Reconstitution.

Storage and Reconstitution

• Storage conditions (per Product Data)

  • Store at −80°C.
  • Avoid repeated freezing and thawing.
  • Shipped on dry ice packs + cold packs.

• Practical handling guidance (general; not item-specific)

  • Upon receipt, promptly transfer to a −80°C freezer. If frequent access is expected, prepare single-use aliquots on ice in a sterile environment to prevent freeze–thaw cycles.
  • Thaw gently on ice; mix by slow inversion. Avoid vigorous vortexing and foaming.
  • Use low-protein-binding tubes and tips to minimize adsorption losses, especially for carrier-free formulations.

• Reconstitution

  • Not specified for this item; refer to CoA/Spec Sheet for any reconstitution instructions, buffer composition, and concentration details.

• Stability after thawing

  • Not specified for this item; users should determine working stability under their specific conditions or consult the datasheet if available.

Always follow institutional best practices for storage of protein biologics and consult the product datasheet for any lot-specific recommendations.

Structure and Identity

This product is a mouse monoclonal antibody directed against UBR7 (ubiquitin protein ligase E3 component n-recognin 7).

• Item-specific identifiers

  • Product Name: UBR7 Mouse mAb (SKU: Ab133358)
  • Grade/Purity: Carrier Free, ExactAb™, Validated (per Product Data)
  • Molecular formula: Not applicable to protein biologics; not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES: Not applicable/not assigned for this biologic; not specified for this item.

• Structural features (general, literature)

  • Antibody class: Mouse monoclonal antibody; exact isotype, light-chain type, and clonality ID are not specified for this item.
  • Typical IgG architecture: 2 heavy chains and 2 light chains linked by disulfides, forming two antigen-binding Fab domains and one Fc domain.
  • Approximate composition: Glycoprotein with N-linked glycans on Fc (pattern depends on production system; not specified for this item).

• 2D “structure in words” (general)

  • Y-shaped immunoglobulin with variable regions forming paratopes that recognize an epitope within UBR7. Constant regions define isotype and effector interactions (not specified for this item).

Notes

• Any sequence, epitope mapping, clone number, and isotype details are not specified for this item; consult the CoA/Spec Sheet if available.

Synthetic Utility

Not applicable. As a biologic macromolecule, this antibody is not employed as a reagent or intermediate in small-molecule synthesis or materials synthesis. Instead, its utility is analytical/affinity-based for detection, capture, and characterization of UBR7 in biological samples.

For functional applications of this reagent in biochemical workflows, see Reaction & Applications and Application Protocols (no item-specific validated applications provided in the current Product Data).

Target Specificity

Information limited to product name and general attributes.

• Antigen/Target: UBR7 (ubiquitin protein ligase E3 component n-recognin 7)
• Antibody type: Mouse monoclonal antibody
• Epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Clone ID: Not specified for this item; refer to CoA/Spec Sheet.
• Isotype/Light chain: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity (tested): Not specified for this item; refer to CoA/Spec Sheet.
• Cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.

Please consult the product datasheet for validation images, sequence context of the immunogen/epitope, and species/application compatibility if available.

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UBR7 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.