UBTD1 Mouse mAb - Primary antibody, specific to UBTD1, Mouse IgG1

Cat. No.: Ab191561

GRADE & PURITY Carrier Free, ExactAb™, Validated, 0.5 mg/mL

Application

WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Clone Number

K9S14

Conjugation

Unconjugated

Overview

Mouse anti Human UBTD1 Antibody, Monoclonal (K9S14), could be used for WB and so on.

Specifications

Product Name

UBTD1 Mouse mAb - Primary antibody, specific to UBTD1, Mouse IgG1

Synonyms

Ubiquitin domain containing 1 | Ubiquitin domain containing protein 1 | Ubiquitin domain-containing protein 1 | UBTD 1 | UBTD1 | UBTD1_HUMAN

Specifications & Purity

ExactAb™, Carrier Free, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

May be involved in the regulation of cellular senescence through a positive feedback loop with TP53. Is a TP53 downstream target gene that increases the stability of TP53 protein by promoting the ubiquitination and degradation of MDM2.

Host species

Mouse

Specificity

UBTD1

Immunogen

Recombinant protein of human UBTD1

Positive Control

WB: A549, HUVEC, U-2 OS, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Product Line

biological_products

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: UBTD1 Mouse mAb (Ab191561)

UBTD1 Mouse mAb (Ab191561) - Western Blot
All lanes: UBTD1 Mouse mAb (Ab191561) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 27, 29 kDa
Exposure time: 39.6 s

Application

Application

Dilution info

WB

1/500-1/1000

Associated Targets(Human)

UBTD1 Tdark Ubiquitin domain-containing protein 1 (0 Activities)

Discover Target: UBTD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Not specified for this item; refer to CoA/Spec Sheet for tested applications (e.g., WB, IHC, IF, IP, ELISA), recommended dilutions, antigen retrieval conditions, sample preparation, and positive control materials.

General reminder: Application performance can vary by sample type, fixation, and detection system; optimize empirically in your laboratory.

Biological Roles

Target background (literature; not product-specific):

• UBTD1 (Ubiquitin domain-containing protein 1) is a conserved ubiquitin-like/UBX-domain protein associated with ubiquitin signaling. It is implicated in modulating E2/E3 ubiquitin ligase interactions and can influence substrate ubiquitination and turnover, intersecting with proteasome-mediated degradation and endoplasmic reticulum–associated degradation (ERAD) pathways.
• Cellular localization: Reports suggest cytosolic localization with potential association to endomembrane compartments where ubiquitin signaling is active. Exact subcellular distribution may be context-dependent and should be confirmed experimentally in the system of interest.
• Functional context: By scaffolding components of the ubiquitination machinery, UBTD1 may impact protein quality control, stress responses, and signaling nodes where regulated proteolysis is integral. Perturbation of UBTD1 expression has been linked to changes in ubiquitination patterns of specific substrates in cellular models.

Relevance for assay design:

• Epitope accessibility can vary across denatured (western blot) versus native (IP/IF) conditions because UBTD1 may participate in complexes. Optimize lysis conditions (detergent strength, salt) to preserve or disrupt complexes as needed.
• Orthogonal confirmation: Pair an anti-UBTD1 mAb with genetic perturbations (siRNA/CRISPR) or orthogonal antibodies targeting non-overlapping epitopes to support specificity.

Note: The above summarizes general biology. Product-specific epitope, clone, isotype, and validated applications are not provided in the product data.

Buffer Applications

Antibodies are not used as buffering agents. For this product, the relevant consideration is compatibility with assay buffers.

General practical guidance for antibody workflows (not product-specific):

• Working buffers: PBS or TBS at pH ~7.2–7.6 are common. Include 0.01–0.05% mild nonionic surfactant to minimize nonspecific adsorption when compatible with the assay. For cell-based applications, ensure isotonicity and compatibility with serum components if applicable.
• Conjugation buffers: For NHS-ester labeling, use amine-free buffers (e.g., PBS or HEPES without primary amines). For maleimide chemistry, maintain mild reducing conditions to generate free thiols on hinge cysteines using controlled reduction protocols.
• Storage buffer: Carrier Free indicates no added BSA or carrier proteins. If long-term storage requires stabilization after your conjugation/workflow, addition of inert protein or trehalose and sterile filtration may be considered in your lab, subject to assay compatibility.

Item-specific buffer compositions, pH, and stabilizers are not specified for this item; refer to CoA/Spec Sheet.

Green Alternatives

Green chemistry substitution among organic solvents or reagents is not directly applicable to a monoclonal antibody product.

Sustainability considerations for antibody use (general guidance):

• Reduce waste: Aliquot to minimize freeze–thaw and extend shelf life, reducing repeat purchases and waste.
• Buffer preparation: Use concentrated stock buffers to minimize packaging and shipping impacts; prepare working solutions on demand.
• Cold-chain optimization: Consolidate orders to reduce shipment frequency of ice-packed materials. Reuse ice packs where possible in compliance with institutional policies.

No direct greener “alternative reagent” applies to this item type.

Pharmaceutical Uses

This product is supplied for research use only. No clinical, diagnostic, or therapeutic uses are claimed or supported.

General context (not product-specific):

• In biopharmaceutical development workflows, research-grade monoclonal antibodies may be used as analytical reagents (e.g., process control assays, target quantitation, mechanism studies) or as surrogates in preclinical discovery experiments. Such uses require separate qualification and are distinct from GMP-manufactured clinical materials.
• Carrier-free antibodies are often preferred for custom conjugation to generate analytical probes (e.g., ADC surrogates, immunoaffinity capture reagents) during early R&D.

For any regulated application, obtain detailed quality documentation and perform in-house qualification. Item-specific pharmacopeial status, residuals, and release tests are not specified for this item.

Physical Properties

Item-specific facts (from Product Data):

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Concentration, buffer, excipients: Carrier Free is specified (no protein stabilizers like BSA; see Quality & Grades). Exact composition and concentration are not specified.
• Molecular weight/formula: Not specified for this item.

General/literature values for antibodies (not product-specific):

• Typical MW: ~150 kDa for full-length IgG; Fab ~50 kDa; scFv ~25–30 kDa.
• Solubility: Highly soluble in aqueous buffers (PBS, TBS) at neutral pH; sensitive to extremes of pH, elevated temperature, and shear.
• Isoelectric point (pI): Commonly ~6–9 depending on sequence/glycosylation; product-specific pI not provided.
• UV properties: Typical IgG A280 extinction coefficient ~1.4 (mg/mL)⁻¹ (literature). Use product-specific E(1%) if provided on CoA.
• Stability: Antibodies generally stable at −20 °C to −80 °C in aliquots; avoid repeated freeze–thaw. Carrier-free proteins may adsorb to untreated plastics; low-bind plastics or addition of inert carrier after conjugation steps can reduce loss.

Note: Numerical specifications such as exact concentration, pH, buffer salts, and additives are not specified for this item; refer to CoA/Spec Sheet for product-specific measurements.

Quality and Grades

Item-specific designations (from Product Data):

• Carrier Free: Supplied without protein carriers (e.g., BSA) or viscosity agents. This enables direct use in conjugation chemistries (enzymatic or chemical), sensitive biophysical assays, or mass spectrometry without carrier background.
• ExactAb™: Platform/brand designation indicating a defined, consistent antibody format. Specific platform details are not provided in the product data; consult CoA/Spec Sheet for expression system, sequence format, and lot-specific metrics.
• Validated: The product is designated as validated by the manufacturer; exact tested applications, sample prep, and performance criteria are not listed in the provided data.

Implications for your workflow:

• Conjugation readiness: Carrier-free format is advantageous for direct labeling (e.g., NHS ester dyes, maleimide linkers following controlled reduction) because carriers can compete or introduce background. Ensure buffer exchange to remove amines or thiols before NHS/maleimide coupling as needed.
• Reproducibility: Sequence-defined or standardized production (as implied by ExactAb™) typically improves lot-to-lot consistency in affinity and background. Confirm with CoA for binding kinetics and purity data (SDS-PAGE, SEC, endotoxin, HCP, residual DNA), which are not specified here.
• Assay compatibility: Absence of carriers reduces interference in ELISA standard curves, SPR/BLI kinetics, and cell-based assays that are sensitive to exogenous proteins.

What is not specified for this item:

• Isotype/subclass, host/expression system, purity by SDS-PAGE/SEC, endotoxin level, preservative content, and tested applications. Refer to CoA/Spec Sheet.

Reaction and Applications

This section pertains to chemical reactions of small molecules (e.g., cross-coupling, Grignard), which are not applicable to monoclonal antibodies.

Relevant applications for this antibody (general guidance, not product-validated):

• Immunoassays: ELISA (capture/detection), western blotting, immunoprecipitation, and immunofluorescence may be feasible application types for a UBTD1-specific mAb; however, specific validated applications for SKU Ab191561 are not provided in the product data.
• Conjugation: Carrier-free format facilitates covalent labeling with enzymes, fluorophores, biotin, or oligos for detection and multiplexing.

Item-specific application validations and conditions are not specified for this item; refer to CoA/Spec Sheet.

Reaction Conditions

Chemical reaction conditions (solvent, temperature, catalysts) are not applicable to this monoclonal antibody.

General handling conditions for biomolecular reactions (not product-specific):

• Temperature: Keep on ice during setup; incubate immunoreactions typically at 4 °C overnight or at room temperature for 1–2 h depending on assay and antibody affinity.
• Buffers: Use near-neutral, isotonic buffers (PBS/TBS). Add blockers (serum, casein, BSA) only if compatible with Carrier Free usage and your downstream application; note that this product is supplied without carriers.
• Detergents/salts: Adjust stringency (e.g., 0.1–0.5% Tween-20; 150–500 mM NaCl) to balance specificity and background in blots/IPs.

Item-specific tested conditions, recommended dilutions, and performance metrics are not specified for this item; refer to CoA/Spec Sheet.

Safety and Handling

Item-specific hazard info (from Product Data):

• GHS classification, pictograms, signal word, H-statements: Not specified for this item; refer to SDS.

General guidance for protein antibodies (not product-specific):

• Expected hazards: Antibody preparations are typically aqueous protein solutions and are generally not classified as hazardous; however, preservatives/additives (not specified here) or azide (if present) can pose risks. Always consult the product SDS for authoritative information.
• PPE: Laboratory coat, gloves, and safety glasses. Handle using good microbiological practices to avoid contamination of the protein and the workspace.
• Handling: Work on ice or at 2–8 °C during setup to minimize denaturation. Avoid vigorous vortexing and foaming. Use low-bind tubes and tips for carrier-free proteins to reduce adsorption.
• Incompatibilities: Avoid strong oxidizers, extreme pH, and organic solvents that denature proteins. Sodium hypochlorite reacts dangerously with sodium azide (if present), forming explosive azides—dispose of azide-containing solutions appropriately. Azide presence is not specified for this item.
• First aid (general): In case of contact with skin or eyes, rinse with water. If inhaled as aerosol or ingested, move to fresh air and seek medical attention if symptoms persist. Spill cleanup with absorbent; decontaminate surfaces.

Always defer to the SDS for storage incompatibilities, accidental release measures, and disposal considerations.

Solvent Selection

Antibody products are aqueous biomolecules rather than small-molecule solutes; conventional organic solvent selection is not typically applicable.

Practical notes for this item type (general guidance):

• Working medium: Use isotonic, near-neutral aqueous buffers (e.g., PBS, TBS) for handling and dilution, unless the CoA specifies a particular buffer system. For conjugation, volatile amine-free buffers (e.g., PBS, HEPES) are preferred for NHS-ester reactions; avoid Tris/glycine when performing NHS chemistry.
• Organic solvents: Avoid appreciable amounts of organic solvents (DMSO, DMF, acetonitrile, alcohols) which can denature proteins. Small volumes may be tolerated during specific conjugations, but validate experimentally.
• Detergents/surfactants: Trace nonionic surfactants (e.g., 0.01% Tween-20) can reduce adsorption losses; do not add unless compatible with your downstream assay. This product is Carrier Free; no surfactants are specified.

For extraction, chromatography, or synthetic solvent selection, see other product categories; for this antibody, buffer selection and gentle handling are the relevant parameters.

Storage and Reconstitution

Item-specific instructions (from Product Data):

• Storage: Store at −20 °C.
• Handling: Avoid repeated freezing and thawing.
• Shipping: Shipped in ice chest with ice pads (cold chain).

Not specified for this item; refer to CoA/Spec Sheet:

• Initial concentration, buffer composition, pH, and any preservatives
• Exact aliquoting guidance and long-term stability claims at −20 °C vs −80 °C
• Reconstitution instructions (if supplied lyophilized) and recommended storage after reconstitution

General best practices (not product-specific):

• Upon receipt, keep cold, inspect for container integrity, and, if in solution, aliquot into low-bind tubes to minimize freeze–thaw.
• Thaw on ice and mix gently; avoid vortexing.
• For carrier-free materials intended for conjugation, ensure buffer compatibility with the selected chemistry before labeling.

Structure and Identity

Item-specific facts (from Product Data):

• Product: UBTD1 Mouse mAb (monoclonal antibody)
• Format/grade: Carrier Free; ExactAb™; Validated
• CAS, CID, InChIKey, SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular formula and molecular weight: Not specified for this item; refer to CoA/Spec Sheet.

General/literature context for monoclonal antibodies (not product-specific):

• Typical architecture: IgG-class antibodies are heterotetramers composed of two heavy and two light chains with disulfide bonds; variable domains define antigen specificity (paratope) directed here toward UBTD1 epitope.
• Approximate size: ~150 kDa for an intact IgG (literature); recombinant fragments (e.g., scFv, Fab) are smaller, but fragment type is not specified for this item.
• Post-translational features: N-linked glycans on Fc (for full-length IgG); exact glycoform depends on expression system (not specified).

Target description (literature):

• UBTD1 (Ubiquitin domain-containing protein 1) is a conserved ubiquitin-like/UBX-domain protein implicated in ubiquitin–proteasome pathway modulation and proteostasis. The antibody recognizes a mouse UBTD1 epitope; precise epitope/clone is not provided in product data.

2D structural description (antibody, conceptual):

• Y-shaped quaternary structure with two identical antigen-binding arms (Fab regions) and one effector Fc stem; complementarity-determining regions (CDRs) within variable domains confer specificity to UBTD1.

Synthetic Utility

Not applicable. This is a protein biologic (monoclonal antibody), not a small-molecule building block or reagent for chemical synthesis.

Relevant laboratory utility (general):

• Immunocapture: Can serve as an affinity reagent to isolate UBTD1 from lysates for downstream proteomics.
• Assay development: Can be incorporated into sandwich ELISAs or proximity assays if paired with compatible anti-UBTD1 reagents recognizing distinct epitopes.

Product-specific conjugation readiness is supported by the Carrier Free designation; however, no specific chemistries or degrees of labeling are provided in the product data.

Target Specificity

Item-specific details (from Product Data):

• Target: UBTD1 (Ubiquitin domain-containing protein 1)
• Species in name: Mouse mAb (monoclonal antibody). Specific clone, epitope, and isotype are not provided.

Not specified for this item; refer to CoA/Spec Sheet:

• Clone ID/name
• Isotype/subclass and light chain type
• Verified species reactivity and cross-reactivity profile
• Epitope mapping or immunogen description
• Affinity/avidity metrics (KD), and blocking/neutralizing capabilities (if any)

General note: Verify target band size and specificity using appropriate positive/negative controls (e.g., UBTD1-overexpressing or knockout lysates) in your system.

Frequently Asked Questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (UBTD1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.