YM Broth - BioReagent

Cat. No.: Y1528395

GRADE & PURITY BioReagent

Storage

Room temperature

Shipped In

Normal

Overview

For cultivation of molds, yeasts, and other acid-resistant bacteria

Specifications

Specifications & Purity

BioReagent

Storage

Room temperature

Shipped In

Normal

Grade

BioReagent

Product Line

biological_products

Application Protocols

No antibody-based or immunoassay application protocols apply to this product.

General microbiological workflow (literature, general):

• Prepare YM Broth per CoA or standard lab protocol. Sterilize and cool.
• Inoculate with a fresh colony or glycerol stock under aseptic conditions.
• Incubate at organism-appropriate temperature with or without agitation.
• For downstream analyses (e.g., enzyme assays, metabolite profiling), harvest cells at defined OD600 or time points.

Item-specific tested applications, recommended dilutions, or positive controls are Not specified for this item; refer to CoA/Spec Sheet.

Biological Roles

YM Broth itself is not a biological molecule but a nutrient matrix designed to support cellular processes in yeasts and fungi.

• Functional role (literature, general):

  • Provides readily assimilable carbon (typically sugars) and nitrogen (peptones) along with vitamins and minerals from yeast/malt extracts, enabling biomass accumulation, fermentation activity, and secondary metabolism.
  • Mildly acidic pH (commonly near 6.2 in typical formulations) favors many yeasts and inhibits excessive bacterial overgrowth relative to neutral media.

• Metabolic support:

  • Carbohydrates fuel glycolysis and fermentation/respiration; peptides and amino acids support protein synthesis.
  • Extract components supply cofactors (e.g., B-vitamins), nucleotides, and trace elements that can reduce the need for complex supplementation.

• Use cases in biochemistry and molecular biology:

  • Production of recombinant proteins in yeast hosts prior to induction in specialized media.
  • Preparation of enzyme-rich cell pastes for biochemical assays.
  • Growth for metabolomics or lipidomics, with awareness that complex media can add background signals; defined media may be preferred for stringent analyses.

Note: Exact growth performance depends on the specific YM formulation, organism, temperature, aeration, and inoculum. For this item, composition and performance parameters are Not specified; consult the CoA/Spec Sheet and perform organism-specific validation.

Buffer Applications

YM Broth is not a defined buffering system. It is a nutrient medium that may exhibit limited inherent buffering due to phosphate and organic components in extracts (literature, general).

• Practical guidance:
  • If precise pH control is required, adjust pH before sterilization and/or supplement with a defined buffer (e.g., citrate-phosphate) compatible with the target organism.
  • Monitor pH drift during growth; yeast metabolism can acidify or alkalinize the medium depending on substrates and aeration.

For dedicated buffer preparation, select a defined buffering agent rather than YM Broth.

Green Alternatives

Environmental considerations for culture media focus on ingredient sourcing, sterilization energy, and waste management rather than solvent replacement.

• Ingredient sourcing (literature, general):

  • Consider plant-derived peptones or certified sustainable yeast/malt extracts to reduce animal-derived inputs and improve supply-chain transparency.
  • Evaluate media that achieve similar growth with lower extract loadings to reduce resource intensity.

• Sterilization choices:

  • Autoclaving (121 °C) is robust but energy-intensive. Where compatible, sterile filtration of heat-labile components and small-batch autoclaving can reduce energy use.

• Waste minimization:

  • Prepare only volumes required for near-term use to limit disposal of expired media.
  • Decontaminate by validated methods (autoclave/chemical) and segregate waste to facilitate appropriate treatment.

• Comparison (general, illustrative):

  • YM Broth vs high-sugar media (e.g., Sabouraud): YM often uses a balanced extract profile that can provide efficient growth at moderate sugar concentration, potentially lowering COD load in effluent compared with very high dextrose formulations.

Trade-offs:

• Lower-temperature sterilization (filtration) is not suitable for complex extracts containing particulates; confirm clarity and filterability.
• Switching to plant-derived peptones may slightly alter growth performance; validate with target strains before adoption.

Always verify that any alternative medium maintains required growth and assay fidelity.

Pharmaceutical Uses

For research use only (per Product Data). Not intended for diagnostic or clinical applications.

In pharmaceutical and biomanufacturing laboratories (literature, general), YM Broth or closely related media may be employed in non-clinical contexts such as:

• Environmental monitoring and method development studies for yeast/fungal recovery (when validated in-house).
• Upstream research on yeast strains used as production hosts, prior to transfer into defined/qualified process media.
• Growth promotion testing in R&D settings to compare medium performance for target organisms.

Notes and cautions:

• For regulated QC/QA testing, only use media that meet the applicable pharmacopeial requirements or validated internal specifications; lot-specific CoAs are essential.
• Extract-based complex media can introduce analytical background in downstream assays; consider defined media for impurity profiling or release testing.

This item’s pharmacopeial status, sterility level, and any growth-promotion certification are Not specified for this item; refer to CoA/Spec Sheet and your quality system requirements.

Physical Properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Miscible with water when prepared as broth (literature, general). Typical dehydrated media readily dissolve in warm water with stirring.
• pH of prepared medium (literature, general): Common YM Broth preparations target pH ~6.2 at 25 °C before sterilization; confirm and adjust per CoA/protocol.
• Density/refractive index/viscosity: Not specified for this item; refer to CoA/Spec Sheet.
• Melting/boiling point: Not applicable as a complex aqueous preparation; dehydrated blend has no discrete melting point (mixture).

Working characteristics (literature, general):

• Typical preparation strength: 20–25 g per liter of purified water, bringing to near-boil to dissolve completely, then sterilizing (see Reaction Conditions and Storage & Reconstitution). This is general guidance; follow the CoA for this product.
• Physical stability: Prepared broth is clear to slightly opalescent pale amber (general observation in literature); may form a light precipitate upon autoclaving depending on water quality and formulation.

All item-specific numeric specifications (e.g., exact pH target, color, and clarity) are Not specified for this item; refer to CoA/Spec Sheet.

Quality and Grades

• Item-specific grade: BioReagent (per Product Data).
  • BioReagent generally denotes suitability for laboratory research, with quality attributes aligned to common biochemical or microbiological workflows.

What BioReagent typically implies (literature, general):

• Raw material quality: Components selected for reproducible growth performance and low interfering contaminants for routine lab assays.
• Performance focus: Lot-to-lot consistency in growth promotion tests using representative yeast and fungal strains, clarity of prepared medium, and pH conformity.
• Documentation: Certificate of Analysis (CoA) typically includes pH range (prepared), appearance, growth promotion results, and microbial limits of the dehydrated product.

Stabilizers/additives:

• Stabilizers are not typically used in dehydrated media; any anti-caking agents or special additives would be disclosed in the CoA. For this item: Not specified for this item; refer to CoA/Spec Sheet.

Compliance notes:

• YM Broth is for research use only (per Product Data). It is not intended for diagnostic or clinical use. If regulatory or QC microbiology use is intended, verify suitability and documentation requirements for your quality system.

Recommendation:

• For critical applications, request or review the lot-specific CoA and, if needed, perform in-house growth promotion testing with your organism panel under intended conditions.

Reaction and Applications

This section is repurposed to highlight laboratory applications of YM Broth in microbiology (not chemical reactions).

Key applications (literature, general):

• Cultivation and maintenance: Supports routine cultivation, propagation, and maintenance of many yeasts (e.g., Saccharomyces spp., Candida spp.) and filamentous fungi that benefit from yeast/malt-derived vitamins and growth factors.
• Biomass production: Useful for generating starter cultures or preparative biomass for downstream biochemical assays, enzyme isolation, metabolite production, or molecular biology workflows.
• Growth characterization: Suitable for growth curve analysis, carbon/nitrogen source utilization studies (with modifications), and physiological profiling under mildly acidic conditions.
• Plating counterpart: Often paired with YM Agar for colony isolation and enumeration; the broth provides a comparable nutrient profile in liquid format.

Practical tips (literature, general):

• Aeration/agitation: Yeasts often benefit from vigorous aeration; use baffled flasks or increased shaking (e.g., 150–250 rpm) for oxygen transfer.
• Inoculum standardization: Start from a fresh colony grown on the corresponding agar; standardize inoculum density (e.g., OD600) for comparability.
• Supplements: Antibiotics, selective agents, or pH adjustments may be added post-sterilization as needed; ensure sterility of additives by filtration.

Note: Formulation and performance specifications for this item are Not specified; refer to the CoA/Spec Sheet for item-specific directions.

Reaction Conditions

This section provides general culture preparation and incubation parameters for YM Broth (literature, general). These are not item-specific specifications; always follow the CoA/Spec Sheet when available.

• Typical preparation (literature): Dissolve approximately 20–25 g of dehydrated YM Broth per liter of purified water with stirring and gentle heat. Adjust pH to ~6.2 at 25 °C if required. Distribute into suitable vessels.
• Sterilization: Autoclave at 121 °C for 15 minutes (standard gravity displacement or pre-vacuum cycle). Cool to ~50–55 °C before adding sterile heat-labile supplements.
• Working volumes and vessels: Use baffled Erlenmeyer flasks at 10–20% fill for aerobic cultures to improve oxygen transfer. For anaerobic/oxygen-limited cultures, seal or use appropriate systems.
• Incubation conditions: Yeasts commonly grow at 25–30 °C; some species at 30–37 °C. Agitation 150–250 rpm on orbital shakers enhances aeration; optimize for strain and vessel geometry.
• Monitoring: Track OD600 for growth curves; plate on YM Agar (or organism-appropriate agar) for CFU enumeration as needed.
• Storage of prepared medium: See Storage & Reconstitution; limit storage time to preserve performance.

Caveats:

• Precipitation after autoclaving may occur with certain waters; filter sterilization (0.22 µm) can improve clarity for optical measurements if the formulation is filterable.
• For selective applications, add antibiotics or pH modifiers post-sterilization; verify compatibility with target organisms.

Safety and Handling

Safety classification (this item):

• Signal Word: Not specified for this item; refer to SDS.
• H-Statements: Not specified for this item; refer to SDS.
• GHS Classification/Pictograms: Not specified for this item; refer to SDS.

General safety considerations for dehydrated culture media (literature, general):

• Hazards: Typically regarded as low hazard; may cause mechanical/irritant effects (dust). Avoid inhalation of powders and contact with eyes.
• PPE: Laboratory coat, safety glasses, and appropriate gloves. Use a dust mask/respirator if handling large quantities of powder or if dust generation cannot be controlled.
• First aid: Rinse eyes with water for several minutes if contacted. Wash skin with soap and water. If inhaled, move to fresh air. If ingested, rinse mouth with water; seek medical advice as needed. Always defer to the product SDS.

Microbiological safety (critical):

• Once inoculated, cultures may contain high titers of viable microorganisms. Treat all used media and cultures as biohazardous material.
• Engineering controls: Use a biological safety cabinet where appropriate. Minimize aerosol generation when pipetting or mixing cultures.
• Decontamination: Autoclave spent media and materials (e.g., 121 °C, ≥15 min) or apply validated chemical disinfectants before disposal.

Storage incompatibilities and handling:

• Store away from strong oxidizers and moisture. Keep container tightly closed to prevent caking/contamination.

Refer to the SDS and institutional biosafety policies for authoritative, use-specific guidance.

Solvent Selection

Not a solvent. YM Broth is an aqueous nutrient medium. Selection considerations instead relate to water quality and sterilization method:

• Water quality (literature, general): Use purified, low-ion, low-organic water (e.g., ASTM Type I–II or equivalent) to minimize precipitates and variability in growth performance.
• Miscibility: Designed for complete dissolution in water with heat and stirring; not intended for use with organic solvents.
• When to choose YM Broth vs alternatives: Select YM Broth for general cultivation of yeasts and many fungi where a mildly acidic, carbohydrate- and peptide-rich medium is desired. Consider YPD, Sabouraud Dextrose, or Malt Extract Broth for organism-specific preferences or methodological requirements.

For solvent properties or extraction workflows, refer instead to the appropriate solvent product category.

Storage and Reconstitution

• Storage conditions (this item): Room temperature (per Product Data). Store tightly closed in a dry place to prevent moisture uptake and caking. Protect from prolonged exposure to humidity and direct heat.

• Shelf life: Not specified for this item; refer to CoA/Spec Sheet.

• Reconstitution/preparation (literature, general):

  • Weigh the recommended amount of dehydrated YM Broth (commonly 20–25 g per liter, per typical YM formulations; confirm with CoA).
  • Add to cold or warm purified water with stirring until dissolved. Gentle heating can expedite dissolution.
  • Adjust pH as needed (typical target ~6.2 at 25 °C in many YM formulations), then dispense into vessels.
  • Sterilize by autoclaving at 121 °C for 15 minutes. Cool to ~50–55 °C before adding sterile, heat-labile supplements or antibiotics if required.
  • For long incubations or sensitive assays, pre-test for clarity and background absorbance/fluorescence.

• Storage of prepared medium (literature, general):

  • Store at 2–8 °C protected from light for short-term use (days to a couple of weeks), minimizing contamination risk. Warm to room temperature before use and mix to re-homogenize. Discard if turbidity, precipitate, or contamination is evident.

• Shipping: Normal (per Product Data).

All item-specific preparation values and stability limits are Not specified for this item; refer to CoA/Spec Sheet. For research use only (per Product Data).

Structure and Identity

YM Broth (Yeast Malt Broth) is a complex, undefined microbiological growth medium formulated to cultivate yeasts and many fungi.

• Item identity (this SKU):

  • Product name: YM Broth (SKU: Y1528395)
  • Grade: BioReagent (per Product Data)
  • Composition details: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES, Molecular Formula, Molecular Weight: Not specified for this item; refer to CoA/Spec Sheet.

• Structural characterization notes:

  • As a complex medium composed of biological extracts and nutrients, YM Broth does not have a single, well-defined molecular structure. It is a mixture typically containing a carbon source, nitrogen source, vitamins, minerals, and growth factors.

• Typical structural features (literature, general):

  • Contains small molecules such as carbohydrates (e.g., glucose/dextrose), peptides/amino acids (from peptone), and complex mixtures from yeast and malt extracts (vitamins, nucleotides, trace minerals).
  • No stereochemical identity applies to the overall mixture; constituents are diverse and partially undefined.

• 2D description in words (general):

  • The prepared medium is an aqueous solution/suspension of low-molecular-weight nutrients and peptides. No discrete ring system or single functional group defines the product; rather, it is a heterogeneous ensemble of saccharides, amino acids/peptides, and extractives.

Note: Exact formulation, lot-specific characteristics (e.g., total nitrogen, carbohydrate content, and pH of prepared medium) should be confirmed against the product’s CoA/Specification Sheet.

Synthetic Utility

Not a synthetic reagent. YM Broth is a biological growth medium and is not typically used as a reagent or intermediate in organic synthesis.

• If chemical synthesis is intended, select appropriate pure solvents and reagents tailored to the reaction class.
• For biocatalysis using yeast, YM Broth can be used to cultivate cells prior to harvesting biocatalyst biomass, but the broth itself is not part of the chemical transformation.

Target Specificity

Not an antibody or affinity reagent; no target specificity, clone, isotype, or species reactivity applies to YM Broth.

• Use-case note: YM Broth supports growth of many yeasts and fungi (literature, general), but it is not selective for a defined biomolecular target.