Z Buffer (For LacZ Staining) - Sterile-filtered, BioReagent

Cat. No.: Z1532750

GRADE & PURITY Sterile-filtered, BioReagent

Synonyms

Miller Z buffer | Yeast Z Buffer | X-gal Staining Buffer | X-gal Development Buffe | β-Galactosidase Assay Buffer

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

1117

Overview

This product is composed of Na₂HPO₄, NaH₂PO₄, KCl, and MgSO₄. It is a buffer at pH 7.0 and has been filter-sterilized. It can be used to prepare Z buffer/X-gal solution for detecting the LacZ reporter gene in the nuclear system AH109 yeast two-hybrid and the membrane system NMY51 yeast two-hybrid.

Z buffer provides a buffering environment for yeast X-gal chromogenic experiments, making yeast colonies develop clearer and more persistent color.

Instructions for Use (using a 5 mL system as an example):

Add 13.5 μL of 2-mercaptoethanol (Aladdin Catalog No.: M755744) and 83.5 μL of X-Gal solution (20 mg/mL) (Aladdin Catalog No.: X1520260) to 5 mL of Z buffer. The preparation can be scaled up proportionally according to the required amount.

Precautions:

1.Pay attention to aseptic operation to avoid microbial contamination.

2.This product is for research use only. Do not use it for pharmaceutical, clinical diagnostic or therapeutic, food, or cosmetic purposes.

3.For your safety and health, please wear a laboratory coat and disposable gloves and a mask during operation.

Specifications

Synonyms

Miller Z buffer | Yeast Z Buffer | X-gal Staining Buffer | X-gal Development Buffe | β-Galactosidase Assay Buffer

Specifications & Purity

Sterile-filtered, BioReagent

Stability And Storage

Store at 2-8℃ long term (18 months).

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, Sterile-filtered

Product Line

biological_products

Names and Identifiers

PH

7