AC-4-130 - 10mM in DMSO , CAS No.1834571-82-2

CAS: 1834571-82-2 Cat. No.: A656586 Formule: C₃₇H₃₆ClF₅N₂O₅S Poids moléculaire: 751.20 PubChem CID: 154701370

GRADE & PURITY 10mM in DMSO

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Vue d’ensemble

AC-4-130 is a potent STAT5 SH2 domain inhibitor. AC-4-130 directly binds to STAT5 and disrupts STAT5 activation, dimerization, nuclear translocation, and STAT5-dependent gene transcription. AC-4-130 induces cell cycle arrest and apoptosis in FLT3-ITD-driven leukemic cells. AC-4-130 has anti-cancer activity and can efficiently block pathological levels of STAT5 activity in acute myeloid leukemia (AML)

In Vitro

AC-4-130 (0.1-100 μM; 72 hours) leads to a significant increase in apoptosis in a dose-dependent and time-dependent manner in MV4-11 or MOLM-13 cells. ?\nAC-4-130 (2, 5 μM; 72 hours) induces cell cycle arrest with an increase in G0/G1 arrested cells and a concomitant reduction in cells in S or G2/M. ?\nAC-4-130 (0.5-2; 24 hours) reveals reduced pY-STAT5 levels both in the cytoplasm and nucleus. ?\nAC-4-130-mediated STAT5 inhibition efficiently blocks the proliferation and clonogenic growth of primary human AML cells, while healthy CD34+ cells are less sensitive. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

IC50& Target:STAT5

Specifications

Spécifications et pureté

10mM in DMSO

Mécanismes biochimiques et physiologiques

AC-4-130 is a potent STAT5 SH2 domain inhibitor. AC-4-130 directly binds to STAT5 and disrupts STAT5 activation, dimerization, nuclear translocation, and STAT5-dependent gene transcription. AC-4-130 induces cell cycle arrest and apoptosis in FLT3-ITD-driv

Conditions de stockage de stockage

Store at -80°C

Expédié en

Dry ice packs + Cold packs

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Noms et identifiants

• PubChem CID: 154701370
• Poids moléculaire: 751.20

Application Protocols

No tested/validated application protocols are provided in the Product Data for AC-4-130.

General guidance for small-molecule screening workflows (not item-specific)

• Stock solution: Prepare 10–50 mM in anhydrous DMSO. Aliquot into low-binding tubes or 384-well source plates. Store at −80°C.
• Serial dilution: Perform in DMSO (e.g., 1:3 or 1:10) to create intermediate plates; back-dilute into assay buffer/media to desired final concentrations, keeping final DMSO constant across wells (commonly 0.1–1% v/v).
• Controls: Include vehicle controls (matching DMSO), positive/negative assay controls, and, where relevant, detergent controls (e.g., 0.01% Tween 20/80) to detect aggregation-based artifacts.
• QC checkpoints: Inspect for precipitation post-dilution; confirm concentrations via LC–UV/LC–MS when quantitative accuracy is critical. Track freeze–thaw events and plate age.

For any application-specific protocol, consult your internal SOPs and validate conditions empirically with this lot.

Biological Roles

No target, pathway, or mode-of-action information is provided in the Product Data for AC-4-130. Consequently, specific biological roles cannot be described for this item.

General considerations for screening compounds (not item-specific)

• Use in exploratory research: Such compounds are often evaluated across biochemical, cell-based, or phenotypic assays to uncover putative targets or pathways of interest.
• Off-target assessment: When activity is observed, follow-up with orthogonal assays and counterscreens is recommended to rule out assay interference (e.g., redox cycling, aggregation). Employ controls such as detergent supplementation and concentration-response curve quality checks.
• Physicochemical properties and bioavailability: If and when structural data become available, assess lipophilicity, ionization state, and polar surface area to anticipate cell permeability and nonspecific binding; verify with empirical measurements.

Compliance note

• For research use only. No claims are made regarding therapeutic, diagnostic, or clinical applications of AC-4-130.

Buffer Applications

This product is not a buffering agent, and no buffer-related specifications are provided. Therefore, dedicated buffer system recipes or pH ranges are not applicable to AC-4-130.

Practical note (general)

• When preparing aqueous working solutions for assays, dissolve the compound first in a suitable organic solvent (commonly DMSO), then dilute into the target buffer immediately before use. Maintain consistent final organic co-solvent percentages across test and control wells to avoid confounding effects.

Green Alternatives

Green chemistry substitution is generally relevant to solvents and bulk reagents. AC-4-130 is supplied as a screening compound; no reagent function is specified. As such, direct “green alternative” substitutions do not apply to the compound itself.

General green practice guidance for handling screening compounds (not item-specific)

• Choose greener solvents for sample preparation and cleanup where feasible (e.g., replace DMF with ethanol or propylene carbonate; replace dichloromethane with ethyl acetate) while maintaining assay performance.
• Minimize DMSO volumes by using concentrated stocks and immediate dilution into aqueous buffers to reduce solvent waste.
• Implement microscale and high-throughput practices to decrease material consumption and waste generation.
• Consolidate shipments on dry ice to reduce packaging, and promptly transfer to ultralow freezers to avoid waste from thawed goods.

Note: Without structure/reactivity information, greener syntheses or process modifications specific to AC-4-130 cannot be proposed.

Pharmaceutical Uses

No pharmacopeial status, excipient role, or formulation guidance is provided for AC-4-130. This item is supplied strictly for research use as part of a small-molecule/compound library and is not intended for therapeutic, diagnostic, or clinical applications.

General formulation context for screening compounds (not item-specific)

• Discovery-stage enabling studies may involve preparing DMSO stock solutions and short-term dosing formulations for in vitro experiments. Any in vivo formulation work requires comprehensive characterization and institutional approvals; no guidance is provided here for AC-4-130.
• Do not infer any pharmacological activity, safety, or efficacy from the product listing. Always generate de novo data under appropriate regulatory and ethical oversight.

Physical Properties

Item-specific physicochemical specifications are not provided in the Product Data for AC-4-130.

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Melting point: Not specified for this item; refer to CoA/Spec Sheet.
• Boiling point: Not specified for this item; refer to CoA/Spec Sheet.
• Density: Not specified for this item; refer to CoA/Spec Sheet.
• Refractive index: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility: Not specified for this item; refer to CoA/Spec Sheet.
• LogP/logD: Not specified for this item; refer to CoA/Spec Sheet.
• pKa: Not specified for this item; refer to CoA/Spec Sheet.
• UV/Vis characteristics: Not specified for this item; refer to CoA/Spec Sheet.

General guidance (literature/common practice for screening compounds; not item-specific)

• Initial dissolution is often attempted in anhydrous DMSO to prepare 10–50 mM stocks; subsequently dilute into assay buffer immediately before use to minimize precipitation.
• If aqueous solubility is anticipated or required, consider co-solvent systems (e.g., DMSO:buffer 1–5% v/v) and evaluate with small-scale tests and visual inspection; verify concentration by UV/Vis or LC where applicable.
• For hygroscopic or air-sensitive compounds (unknown for this item), minimize exposure to ambient moisture and oxygen during handling.

Always consult the item’s CoA/Spec Sheet for authoritative, lot-specific physical property information.

Quality and Grades

• Grade/Purity: Not specified for this item; refer to CoA/Spec Sheet.
• Stabilizers/added components: Not specified for this item; refer to CoA/Spec Sheet.

Guidance on interpretation (general; not item-specific)

• Screening compound/library grade typically emphasizes identity confirmation (e.g., LC–MS, HRMS, NMR) and purity sufficient for biochemical/cellular assays (commonly ≥90% by HPLC, but verify on CoA). Low-UV-absorbing solvent residues are preferred for analytical compatibility.
• If a salt form or solvate is supplied, the effective free-base/free-acid content influences stock solution molarity; confirm counterion/solvate content on the CoA.
• UV cutoff, residual solvents, water content (KF), and metal/peroxide limits are critical for some assays. When not listed on the CoA, perform in-house verification if your application is sensitive to these parameters.

Documentation

• For batch-specific purity, analytical traces (HPLC/LC–MS), and identity data, consult the corresponding CoA/Spec Sheet. These documents are the authoritative source for acceptance criteria and release testing for this item.

Reaction and Applications

No synthetic/reagent applications are specified for AC-4-130 in the Product Data. In the absence of a disclosed structure, this product should be considered a research screening compound rather than a general-purpose synthetic reagent.

General research uses for screening compounds (not item-specific)

• Biochemical and cell-based screening: Preparation of DMSO stocks for target-agnostic primary screening, hit validation, and mechanism-of-action follow-up studies.
• Analytical characterization: Method development and stability-indicating assays (LC–UV/LC–MS) to assess compound integrity in storage and assay-relevant matrices.
• Physicochemical profiling: Determine solubility, lipophilicity (logD), and microsomal/chemical stability as part of early ADME profiling (no clinical claims).

Good practices

• Validate identity and purity upon receipt against the CoA (e.g., HPLC, MS). If the structure is later disclosed, orthogonal analytical methods (1H/13C NMR, HRMS) are recommended.
• Minimize freeze–thaw cycles of DMSO stocks; aliquot into assay-ready vials or plates. Track plate map, DMSO percentage, and dilution history to ensure data integrity.
• Record and control water content in DMSO if hydrolysis-sensitive motifs are suspected; use anhydrous solvents and desiccated storage where appropriate.

If you intend to use AC-4-130 as a building block or reagent, obtain the detailed structure and reactivity profile first; otherwise, such applications are not recommended.

Reaction Conditions

Item-specific reaction conditions are not applicable because AC-4-130 is supplied as a screening compound with no disclosed structure or reagent role in the Product Data.

General analytical and handling conditions for screening compounds (not item-specific)

• Stock preparation: 10–50 mM in anhydrous DMSO, aliquoted to avoid freeze–thaw. Vortex and, if necessary, sonicate briefly to aid dissolution. Filter (0.2 µm PTFE) if particulate persists.
• Assay dilution: Add to assay buffer/media to achieve target final concentration with matched DMSO percent across controls and treated samples; verify absence of precipitation and adsorption losses.
• Stability checks: Establish bench stability (e.g., 4–24 h at room temperature in assay buffer) by LC–UV/LC–MS. Evaluate light sensitivity with foil-wrapped vs. exposed controls if aromatic/photolabile motifs are suspected once structural data are available.

If synthetic reactions are intended, first obtain structure and reactivity information; otherwise, no reliable reaction parameters can be recommended.

Safety and Handling

Authoritative safety data (GHS classification, pictograms, H-statements) are not provided in the Product Data for AC-4-130.

• GHS/CLP classification: Not specified for this item; refer to SDS.
• Signal word: Not specified for this item; refer to SDS.
• Hazard statements: Not specified for this item; refer to SDS.

General laboratory safety guidance (not item-specific; follow your institutional EHS policies and the SDS)

• PPE: Wear lab coat, safety glasses, and appropriate chemically resistant gloves. Use in a fume hood to avoid inhalation of vapors/aerosols.
• Avoid exposure: Prevent contact with skin and eyes. Do not ingest or inhale. Wash thoroughly after handling.
• Incompatibilities: Unknown for this item. As a precaution, segregate from strong oxidizers/reducers and reactive acids/bases until compatibility is confirmed via SDS.
• First aid (overview): In case of skin/eye contact, rinse with water for at least 15 minutes; remove contaminated clothing. If inhaled, move to fresh air. If ingested, rinse mouth; seek medical attention. Always consult SDS for specific measures.
• Spill response: Absorb small spills with inert material (e.g., vermiculite), collect in appropriate waste container. Ventilate area. Decontaminate surfaces per SDS guidance.
• Waste disposal: Dispose of contents/container in accordance with local/regional regulations; many screening compounds are handled as hazardous organic waste.

For research use only. Not for human or veterinary use. Always defer to the product’s SDS for authoritative safety and handling information.

Solvent Selection

Item-specific solubility and polarity data are not provided for AC-4-130. The following is general, literature-based guidance for small-molecule screening compounds (not item-specific):

• Primary solvent for stock solutions: Anhydrous DMSO is the default for many discovery compounds due to broad solubilizing power and assay compatibility at low final percentages (typically 0.1–1% v/v in biochemical/cellular assays).
• Secondary solvents: DMF, ethanol, methanol, acetonitrile can be evaluated for analytical sample prep. For biological assays, ethanol or PEG400 co-solvent systems may be considered with appropriate controls.
• Aqueous work: If aqueous solubility is anticipated, use buffered solutions at the target assay pH and include a minimal DMSO co-solvent (1–5% v/v) to stabilize supersaturated stocks. Verify absence of precipitation after dilution by visual inspection and analytical check (e.g., UV/Vis baseline scatter, LC).
• Surfactants: For notoriously insoluble chemotypes, nonionic surfactants (e.g., 0.01–0.1% Tween 80 or Pluronic F-68) can aid dispersion in cell-based assays. Validate for assay interference.

Practical selection tips

• Start with 10–50 mM DMSO stock; perform a solubility ladder (serial dilution in intended matrix) and record cloud points.
• Avoid prolonged storage of dilute aqueous solutions; prepare fresh working solutions immediately before use to reduce adsorption loss and hydrolysis risk.
• When analytical quantitation is required, confirm concentration by LC–UV or LC–MS against standards.

Storage and Reconstitution

Item-specific conditions from Product Data

• Storage: Store at −80°C.
• Shipping: Shipped on dry ice packs + cold packs.

Additional best practices (general; not item-specific)

• Upon receipt: Keep the package cold, inspect contents immediately, and transfer the item to a −80°C freezer. Avoid accidental thawing.
• Aliquoting: If supplied as a solid, subdivide rapidly in a cold, dry environment (preferably under inert gas for moisture/oxygen-sensitive materials; sensitivity unknown for this item). If supplied in solution, dispense into single-use aliquots to minimize freeze–thaw cycles.
• Reconstitution: If solubility data are unavailable, begin with anhydrous DMSO to prepare a 10–50 mM stock. Mix thoroughly (vortex/brief sonication). If aqueous working solutions are required, dilute the DMSO stock into buffer/media immediately before use. Verify clarity and absence of precipitation.
• Stability: Limit exposure to ambient temperature and light. Record preparation date, solvent, and concentration on each aliquot. Discard aliquots showing discoloration, precipitate, or off-odor.
• Freeze–thaw: Avoid repeated freeze–thaw; use single-use aliquots. Store aliquots desiccated and protected from light when applicable.

For exact specifications (purity, counterion, water content, recommended solvents), consult the lot-specific CoA/Spec Sheet.

Structure and Identity

• Product name: AC-4-130 (SKU: A656586); CAS: 1834571-82-2; PubChem CID: 154701370
• Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
• Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• SMILES: Not specified for this item; refer to CoA/Spec Sheet.
• InChIKey: Not specified for this item; refer to CoA/Spec Sheet.
• Synonyms: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features: Functional groups, ring systems, and stereochemistry are not available in the provided data. A 2D structural description cannot be supplied without the structure; consult the CoA/Spec Sheet or the referenced CID if available within your organization’s approved resources.
• Category: Small molecules and compound libraries (screening compound).

Notes

• Item-specific identifiers provided above are the only authoritative identifiers from the Product Data. Any additional structural details should be verified against the item’s CoA/Spec Sheet prior to experimental design.

Synthetic Utility

Synthetic roles (e.g., building block, coupling partner, reagent) cannot be assigned to AC-4-130 because the structure and functional groups are not provided in the Product Data. Consequently, guidance on named reactions or retrosynthetic applications would be speculative and is intentionally omitted.

If you plan to repurpose this material for synthesis

• Obtain the complete structural information and verify functional group content via CoA/NMR before attempting any transformation.
• Perform a small test reaction with analytical follow-up (LC–MS, NMR) to establish baseline stability under the intended conditions (acid/base, redox, thermal, photochemical).
• Document salt/stoichiometry if a salt or solvate form is involved to ensure accurate molarity and yield calculations.

Target Specificity

Target, pathway, or binding specificity data are not provided for AC-4-130.

• Target/epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity/selectivity: Not specified for this item; refer to CoA/Spec Sheet.
• Assay-validated activity: Not specified for this item; refer to CoA/Spec Sheet.

Until definitive data are available, AC-4-130 should be treated as a target-agnostic screening compound.

Foire aux questions

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 1834571-82-2, the molecular formula is C37H36ClF5N2O5S, and the molecular weight is 751.20 g/mol.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

How should this product be stored?

Store at ?80 °C. Ultra-low temperature storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.