ANAPC7 Mouse mAb - Primary antibody, specific to ANAPC7

Cat. No.: Ab327499

GRADE & PURITY KD Validation

Application

WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG1

Conjugation

Unconjugated

Specifications

Product Name

ANAPC7 Mouse mAb - Primary antibody, specific to ANAPC7

Spécifications et pureté

KD Validation

Espèce hôte

Mouse

Spécificité

ANAPC7

Immunogène

Recombinant protein of human ANAPC7

Conjugation

Unconjugated

Grade

KD Validation

Méthode de purification

Protein G purified

Product Line

biological_products

Stockage et expédition

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at -20°C for one year.

Application Protocols

• Item-specific (Product Data)

  • Tested/validated applications, recommended dilutions, incubation times, positive controls, and antigen retrieval conditions: Not specified for this item; refer to CoA/Spec Sheet.

• General protocol outlines (literature/practice; not item-specific)

  • Western blot (outline): Prepare lysates with protease inhibitors; denature/reduce as appropriate; transfer to PVDF; block (e.g., 5% milk in TBST); incubate with primary antibody (optimize dilution); wash; incubate with species-matched secondary; detect via chemiluminescence.
  • Immunoprecipitation: Incubate clarified lysate with antibody; capture with Protein A/G resin; wash; elute under mild conditions for complex preservation or denaturing conditions for WB.
  • Immunofluorescence: Fix (paraformaldehyde), permeabilize (Triton X-100 if needed), block (BSA/serum), primary incubation, wash, fluorescent secondary incubation, mount with anti-fade.

These outlines are provided for planning purposes only. For this specific item, rely on the CoA/Spec Sheet for any tested applications and detailed directions.

Biological Roles

• Target overview (literature/general; not item-specific)

  • ANAPC7 encodes a tetratricopeptide repeat (TPR) subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase that marks key cell-cycle regulators (e.g., securin, cyclins) for proteasomal degradation, enabling metaphase–anaphase transition and mitotic exit.
  • Functional context: TPR subunits (ANAPC7, ANAPC3/CDC27, ANAPC6/CDC16) scaffold co-activators CDC20 and CDH1 and help recruit substrates via degrons (D-box, KEN-box). Proper APC/C function ensures chromosome segregation fidelity and prevents aneuploidy.
  • Localization: Predominantly cytoplasmic with centrosomal/mitotic spindle association reported during cell division (literature), but distribution can vary by cell type and cell-cycle stage.
  • Regulation: Activity and substrate selection depend on spindle assembly checkpoint signaling, phosphorylation state of APC/C components, and co-activator binding.

• Research relevance (general)

  • ANAPC7 detection supports studies on cell-cycle checkpoints, proteostasis, and ubiquitin-mediated degradation. Perturbations in APC/C components are studied for their roles in genome stability.

• Item-specific

  • This antibody is intended for research use only (Product Data). Species reactivity, epitope, and validated assay types are Not specified for this item; refer to CoA/Spec Sheet.

Buffer Applications

This item is an antibody reagent; it is not a buffering agent and is not used to establish defined pH systems.

• Item-specific (Product Data)

  • Supplied buffer and recommended diluents: Not specified for this item; refer to CoA/Spec Sheet.

• Practical guidance (general)

  • For assay buffers (WB/IF/ELISA), commonly used systems include PBS (pH ~7.4) or TBS (pH ~7.4–8.0) with additives tailored to the application (e.g., 0.05% Tween-20 for washes; 1–5% BSA or non-fat dry milk for blocking). Optimize based on background and antigen exposure.

If you require a defined buffering system for your workflow, select from dedicated buffer products; this antibody should be diluted into compatible assay buffers rather than formulated to buffer pH itself.

Green Alternatives

Antibody reagents are aqueous biologics; green chemistry considerations focus on minimizing hazardous additives, waste, and energy use rather than substituting organic solvents.

• Item-specific (Product Data)

  • Preservatives/excipients: Not specified for this item; refer to CoA/Spec Sheet.

• Greener practice recommendations (general)

  • Buffer selection: Use phosphate- or tris-based buffers prepared with deionized water; avoid unnecessary organic co-solvents.
  • Stabilizers: Prefer benign stabilizers (e.g., trehalose, low % nonionic surfactant) where compatible with downstream assays to reduce need for cold-chain extremes.
  • Aliquoting: Prepare single-use aliquots to prevent freeze–thaw–induced losses, reducing repeat experiments and waste.
  • Energy use: Store at −20°C as specified; avoid unnecessary −80°C storage if not required by the CoA.
  • Waste reduction: Scale assays appropriately, adopt micro-volume formats, and use reusable, validated labware where sterility is not compromised.

• Comparative note (general)

  • Sodium azide vs thimerosal: If preservatives are needed, azide is effective but incompatible with peroxidase-based assays and hazardous for wastewater microbes; thimerosal has mercury content. Where possible, use preservative-free formulations and maintain sterility through good aseptic technique. Consult the CoA to confirm what this item contains.

Pharmaceutical Uses

This product is supplied strictly for research use only and is not intended for human or veterinary use, diagnostics, or therapeutic applications (Product Data: Research Use Only).

• Item-specific (Product Data)

  • Regulatory/compendial status: Not specified for this item; refer to CoA/Spec Sheet.

• General context for monoclonal antibodies (not item-specific)

  • In pharmaceutical manufacturing, research-grade antibodies may be used in process development assays (e.g., cellular analytics, target quantitation) but are segregated from GMP-grade reagents.
  • Excipient and formulation details (buffers, stabilizers, preservatives), sterility/endotoxin specifications, and viral safety validation are required for clinical/GMP contexts and are not provided for this research reagent.

• Practical note

  • Do not use this product as an excipient, API, or in any clinical workflow. For regulated applications, obtain GMP-grade, fully qualified reagents with lot-specific quality documentation.

Physical Properties

• Item-specific (Product Data)

  • Appearance, concentration, buffer composition, excipients: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight/form: Not specified for this item; refer to CoA/Spec Sheet.

• Literature/general values for antibodies (not item-specific)

  • Typical IgG molecular mass: ~150 kDa (monomer) comprised of ~50 kDa heavy chains and ~25 kDa light chains.
  • Isoelectric point (pI): Commonly ~6–9 depending on subclass and sequence (literature range).
  • Solubility: Highly soluble in aqueous buffers (PBS, TBS) across neutral pH; stability influenced by ionic strength and excipients (e.g., sugars, amino acids).
  • Stability considerations: Sensitive to repeated freeze–thaw; aggregation risk increases at elevated temperature, low ionic strength, or extreme pH.
  • UV characteristics: Proteins exhibit A280 absorption due to Trp/Tyr; typical IgG extinction coefficient ~1.35–1.6 (mg/mL)−1 cm−1 (literature, sequence dependent).

• Not applicable properties for a biologic

  • Boiling point, melting point, vapor pressure, logP, SMILES/InChI-based descriptors do not apply to macromolecular antibodies.

Note: For precise item specifications (concentration, buffer, excipients, preservatives, optical density), consult the product CoA/Spec Sheet; values above are general literature guidance only.

Quality and Grades

• Item-specific (Product Data)

  • Grade/Purity: KD Validation.

• Interpretation of “KD Validation” (general explanation; not a guarantee beyond Product Data)

  • KD (knockdown) validation typically indicates the antibody’s specificity was evaluated by comparing signal in control samples versus samples where the target gene (here, ANAPC7) was reduced via RNAi/shRNA/siRNA or CRISPR interference. A specific antibody should show a diminished band/staining in the knockdown condition.
  • Benefits: Increases confidence in target specificity, reduces risk of off-target binding, and supports use in knockdown/knockout controls.
  • Scope: KD validation is one dimension of validation; performance can be application- and species-dependent.

• What this means for this item

  • The listing identifies KD Validation as the grade. Application-specific performance metrics (WB/IHC/IF/ELISA), isotype/subclass, epitope, and species cross-reactivity are Not specified for this item; refer to CoA/Spec Sheet.

• Practical implications for users

  • Controls: Incorporate positive controls (ANAPC7-expressing lysates) and negative controls (KD/KO or peptide block, if available) to mirror KD-validation logic in your system.
  • Documentation: Check the lot-specific CoA for concentration, preservative, and recommended dilutions prior to setting up assays.
  • Traceability: Record lot numbers; performance may vary between lots despite validation category.

Reaction and Applications

Classical organic/inorganic reaction chemistry does not apply to monoclonal antibodies. For this product, “applications” refers to immunodetection/affinity workflows.

• Item-specific (Product Data)

  • Manufacturer Applications: Not specified in the listing beyond KD Validation; refer to CoA/Spec Sheet for tested applications.

• General applications for a mouse monoclonal anti-ANAPC7 (literature/practice; not item-specific)

  • Western blotting (WB): Detect ANAPC7 in denatured cell lysates; include molecular weight markers and KD/KO controls when possible.
  • Immunoprecipitation (IP): Enrichment of ANAPC7 and associated APC/C components for interaction studies.
  • Immunofluorescence (IF)/Immunocytochemistry (ICC): Cellular localization during cell cycle phases; fixation/permeabilization conditions can affect epitope exposure.
  • ELISA: If antigen is available in native/coated form, sandwich or indirect formats may be developed.

• Practical tips (general)

  • Antigen retrieval: If performing IHC on FFPE tissues, test heat-induced epitope retrieval buffers (citrate vs EDTA) to optimize signal.
  • Controls: Always include isotype control, secondary-only control, and KD/KO control to verify specificity.
  • Load/amount: Start titrations over a broad range (e.g., 1:500–1:5,000 for WB) and optimize empirically; item-specific recommendations are not provided here.

Reaction Conditions

Conventional reaction conditions (temperature, solvent, catalysts, yields) are not applicable to an antibody reagent.

• Item-specific (Product Data)

  • Tested application conditions and recommended dilutions: Not specified for this item; refer to CoA/Spec Sheet.

• General assay conditions for antibodies (not item-specific)

  • Western blot: Typical starting dilutions 1:500–1:5,000 in TBST with 1–5% BSA or milk; incubate 1–16 h at 4–25°C; optimize empirically.
  • Immunofluorescence/ICC: 1:100–1:1,000 in PBS/BSA with permeabilization (e.g., 0.1–0.3% Triton X-100) if required; avoid over-fixation that masks epitopes.
  • Immunoprecipitation: 1–5 µg antibody per mg lysate protein; incubate 1–4 h at 4°C with Protein A/G support; wash stringency adjusted to preserve complexes.
  • ELISA: Coating 0.5–2 µg/mL antigen; detection antibody titrated for desired dynamic range.

All values above are literature/practice guidance, not specifications for this item. Validate conditions in your system and consult the CoA for any lot-specific recommendations.

Safety and Handling

• Item-specific (Product Data)

  • GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to SDS.
  • Storage: Store at −20°C; avoid repeated freezing and thawing (Product Data).
  • Shipping: Shipped in ice chest with ice pads (Product Data).

• General safety guidance for antibodies (literature/practice)

  • PPE: Laboratory coat, gloves, and eye protection when handling liquids or lyophilized material.
  • Hygiene: Work in a clean area to avoid microbial contamination. Use low-protein binding tips/tubes to minimize loss and aerosol generation.
  • Incompatibilities: Avoid extreme pH (<5 or >9) and strong oxidants; limit surfactant-free vigorous mixing that can induce aggregation/foaming.
  • Freeze–thaw risk: Repeated cycles accelerate aggregation and activity loss. Prepare single-use aliquots on first thaw.
  • Biological origin caution: Treat as potentially bioactive protein; avoid ingestion/injection. If of cell culture origin, consider universal precautions even if non-hazardous by GHS.

• First-aid overview (defer to SDS for authoritative instructions)

  • Skin/eye contact: Rinse with water for several minutes. Remove contaminated clothing.
  • Inhalation of aerosols: Move to fresh air.
  • Ingestion: Rinse mouth; seek medical advice.

Always refer to the product’s SDS for definitive hazard, spill, disposal, and first-aid procedures.

Solvent Selection

This product is an aqueous protein reagent rather than a small-molecule solute; classical solvent selection is not typically applicable.

• Item-specific (Product Data)

  • Formulation/buffer: Not specified for this item; refer to CoA/Spec Sheet.

• General guidance for antibody handling (literature/practice)

  • Preferred media: Neutral aqueous buffers such as PBS or TBS, commonly with stabilizers (e.g., 0.1–1% BSA) and low levels of nonionic surfactant (e.g., 0.01% Tween-20) to minimize adsorption and aggregation. Only adjust if compatible with your assay and if not already included.
  • Avoid: Organic solvents, strong acids/bases, and high concentrations of chaotropes unless deliberately performing elution or regeneration steps.
  • Diluent choice: Use the same buffer matrix as the stock where possible; if unknown, start with PBS, pH ~7.4, and assess stability.

For solvent-like decision points (e.g., blocking/dilution buffers), select based on assay (WB vs IF vs IHC) and background profiles rather than classical polarity or dielectric considerations.

Storage and Reconstitution

• Item-specific (Product Data)

  • Storage: Store at −20°C. Avoid repeated freezing and thawing.
  • Shipping: Ice chest with ice pads.
  • Form/concentration/buffer: Not specified for this item; refer to CoA/Spec Sheet.

• General guidance (not item-specific)

  • Upon receipt: If lyophilized, briefly centrifuge the vial before opening to collect contents. Reconstitute with sterile, neutral pH buffer (e.g., PBS) to the desired concentration, gently invert to mix; avoid vigorous vortexing. If liquid, verify buffer composition on CoA.
  • Aliquoting: Prepare single-use aliquots in low-protein-binding tubes immediately after first thaw/reconstitution. Avoid more than one freeze–thaw cycle per aliquot.
  • Storage temperatures: Short-term (≤1–2 weeks) at 2–8°C may be acceptable for many antibodies; for longer-term, follow the specified −20°C storage. Do not store at room temperature.
  • Stabilizers: Addition of carrier protein (e.g., 0.1% BSA) and cryoprotectants (e.g., 10–50% glycerol) can improve stability at −20°C; only apply if compatible with your assays and if not already present. Confirm with CoA before modifying formulation.
  • Light and contamination: Protect from light if conjugated (label not specified for this item) and maintain aseptic technique to prevent microbial growth.

Always consult the lot-specific CoA/Spec Sheet for exact reconstitution volumes, final concentrations, and any preservative/excipient information for this item.

Structure and Identity

• Item-specific (Product Data)

  • Product: ANAPC7 Mouse mAb (SKU: Ab327499)
  • Type: Monoclonal antibody, host species: Mouse
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
  • Sequence/clone/isotype: Not specified for this item; refer to CoA/Spec Sheet.
  • CAS, CID, InChIKey, SMILES: Not applicable for protein biologics; not assigned.

• Literature/general information (not item-specific)

  • Typical IgG structure: Two heavy chains and two light chains forming a Y‑shaped heterotetramer with disulfide bonds; variable domains confer antigen specificity; Fc mediates effector functions.
  • Approximate molecular mass: ~150 kDa for a full-length IgG (literature).
  • Target description: ANAPC7 (Anaphase-Promoting Complex subunit 7) is a tetratricopeptide repeat (TPR) protein component of the APC/C E3 ubiquitin ligase complex involved in metaphase–anaphase transition and mitotic exit.

• 2D/structural description in words (general)

  • Antibody domains: Each heavy and light chain contains variable (V) and constant (C) domains; antigen-binding sites are formed by the six CDR loops at the tips of the Fab arms. The antibody recognizes an epitope on ANAPC7; epitope mapping not specified for this item.

Synthetic Utility

This product is a monoclonal antibody, not a small-molecule building block or catalytic reagent.

• Not applicable to this item

  • It does not participate in classical organic synthesis, polymerization, or metal-catalyzed transformations.

• Relevant laboratory utility (general)

  • Affinity capture: Can be used in immunoprecipitation or pull-down assays to enrich ANAPC7 and associated APC/C complexes for downstream MS or immunoblotting.
  • Detection: Supports antigen detection in WB/IF/IHC/ELISA when compatible with assay conditions.

• Item-specific constraints

  • Application scope, binding epitope, and tested species are Not specified for this item; refer to CoA/Spec Sheet for any validated uses.

For synthetic chemistry applications, consider small-molecule reagents or catalytic antibodies specifically engineered for catalysis; this reagent is intended for analytical/biochemical workflows.

Target Specificity

• Item-specific (Product Data only)
  • Target antigen: ANAPC7 (Anaphase-Promoting Complex subunit 7)
  • Antibody type: Mouse monoclonal antibody (mAb)
  • Clone ID: Not specified for this item; refer to CoA/Spec Sheet.
  • Epitope: Not specified for this item; refer to CoA/Spec Sheet.
  • Species reactivity: Not specified for this item; refer to CoA/Spec Sheet.
  • Isotype/subclass: Not specified for this item; refer to CoA/Spec Sheet.
  • Conjugate/label: Not specified for this item; refer to CoA/Spec Sheet.
  • Cross-reactivity/competition data: Not specified for this item; refer to CoA/Spec Sheet.

No additional specificity claims are made beyond the product name and KD Validation grade; consult the lot-specific documentation for detailed validation data.