APT Broth - BioReagent

Cat. No.: A1528504

QUALITÉ & PURETÉ BioReagent

Storage

Room temperature

Shipped In

Normal

Vue d’ensemble

For cultivation of heterofermentative Lactobacillus and other bacteria requiring high levels of thiamine hydrochloride

Spécifications

Spécifications et pureté

BioReagent

Conditions de stockage de stockage

Room temperature

Expédié en

Normal

Grade

BioReagent

Product Line

Produits biologiques

Application Protocols

Item-specific, tested application protocols are not provided for this SKU. Refer to institutional SOPs and the Reaction Conditions tab for general, literature-based preparation and incubation guidance.

For detailed protocols (e.g., enumeration, MIC testing, or anaerobic cultivation) adapt standard methods (ISO/USP/ASTM/CLSI) to your organism and study design, and qualify locally.

Biological Roles

APT Broth functions as a supportive environment for microbial growth rather than exerting a specific biochemical activity. The following roles describe the nutritional ecology of typical APT-type formulations (literature/general):

• Carbon and energy: Fermentable carbohydrates (e.g., dextrose) fuel glycolysis and lactic fermentation pathways in LAB, yielding lactic acid and regenerating NAD+.
• Nitrogen and growth factors: Peptones/tryptone and yeast extract supply amino acids, small peptides, B vitamins, and nucleotides, reducing biosynthetic burden on fastidious organisms.
• Lipid and membrane components: Polysorbate (Tween) can supply fatty acids and improve uptake of hydrophobic nutrients for organisms with limited fatty acid biosynthesis.
• Buffering and ions: Phosphate/citrate buffers stabilize pH near neutrality; Mg2+, Mn2+, and Fe2+ serve as enzyme cofactors (e.g., for lactate dehydrogenase and superoxide dismutase).
• Redox considerations: Some components lower redox potential, which can be favorable for aerotolerant anaerobes.

These biological roles explain the broad utility of APT-type media for cultivating lactic acid bacteria and other nutritionally demanding microbes in research workflows.

Buffer Applications

APT Broth is a growth medium rather than a dedicated buffer. However, typical APT-type formulations include phosphate and/or citrate salts that provide moderate buffering capacity around neutral pH (literature/general):

• Effective pH range (prepared medium): approximately pH 6.5–7.0, supporting lactic acid bacteria while accommodating acidification during growth. Exact pH for this SKU is not specified; refer to CoA/Spec Sheet.
• Practical guidance:
  • For experiments sensitive to pH drift (e.g., metabolic flux studies), supplement with additional buffering (e.g., 50–100 mM MOPS or phosphate) or use controlled bioreactors with pH-stat.
  • Monitor and record starting pH after sterilization; small shifts may occur during autoclaving.

If your primary need is a buffer (not growth), choose a defined buffering system (e.g., phosphate, MOPS, HEPES) rather than a nutrient medium.

Green Alternatives

While APT Broth itself is a nutrient medium (not a hazardous solvent), sustainable practices can reduce environmental impact.

• Comparison of media choices (literature/general):
  • APT vs. MRS: Both are biodegradable. Select the medium that achieves growth with the least preparation and incubation time to lower energy and resource usage.
  • APT vs. custom-defined media: Chemically defined media can reduce lot-to-lot variability and waste in some studies, but may require more complex supplement preparation.

Sustainability practices:

• Prepare only the volume needed; unused sterile broth has a finite shelf life and increases autoclave energy use if discarded.
• Use energy-efficient sterilization cycles and load autoclaves fully when institutional policy allows to maximize throughput per cycle.
• Employ reusable glassware where practical; verify cleaning validation to avoid inhibitory residues.
• Dispose of spent media via autoclaving and sewer discharge only if permitted by local regulations; otherwise, treat as biohazardous waste. Avoid adding hazardous chemicals to media waste streams.

Item-specific environmental certifications or green attributes: Not specified for this item; refer to CoA/Spec Sheet.

Pharmaceutical Uses

No therapeutic or clinical claims are made for this product. Research-use-only medium.

Relevant research and manufacturing support roles (literature/general):

• Microbial quality assessments in R&D settings: cultivation and enumeration of lactic acid bacteria from raw materials, in-process samples, or environmental monitoring (non-GMP contexts unless otherwise qualified).
• Growth promotion testing in microbiology laboratories to verify performance of test organisms relevant to probiotics research.
• Upstream research on microbial production strains or consortia where lactic acid bacteria act as helper/adjunct organisms.

Regulatory/compendial status for this specific SKU: Not specified for this item; refer to CoA/Spec Sheet. For regulated workflows, users should qualify the medium per internal SOPs and applicable pharmacopeial guidance before use.

Physical Properties

• Appearance: Not specified for this item; refer to CoA/Spec Sheet.
• Solubility (literature, general for dehydrated media):
  • Readily soluble in purified water to form a clear to slightly opalescent broth after sterilization.
• pH (literature, prepared broth at 25 °C):
  • Typical range for APT-type media: ~6.7–6.9 before sterilization; may shift slightly after autoclaving. This is general literature guidance, not an item specification.
• Density, refractive index, logP, BP/MP: Not applicable/Not measured for complex media; not specified for this item.
• Osmolality/ionic strength (literature):
  • Moderate, primarily due to phosphate/citrate buffers and sodium salts; exact values are formulation-dependent and not specified here.

Practical notes (general, literature):

• Dissolution is improved by gradually sprinkling the powder into vigorously stirred water heated to ~40–50 °C; avoid boiling prior to sterilization to prevent Maillard reactions/darkening.
• Final appearance after sterilization is usually clear to slightly hazy; excessive haze can indicate overdosing, incomplete dissolution, or precipitation of divalent salts.

Quality and Grades

• Item-specific grade: BioReagent (as provided in Product Data).
• What BioReagent typically implies (general explanation):
  • Manufactured to meet performance expectations for research use in the life sciences (e.g., reproducible growth characteristics, low bioburden of the dehydrated powder).
  • Batch-to-batch consistency suitable for microbiology workflows, media preparation, and routine QC of organisms relevant to research. Not produced to clinical diagnostic or GMP manufacturing specifications unless explicitly stated.
• Purity/stabilizers for this SKU:
  • Not specified for this item; refer to CoA/Spec Sheet.
• Documentation and lot control (recommended practice):
  • Refer to the lot-specific Certificate of Analysis for growth promotion test results, recommended preparation parameters (g/L), and any performance verification data.
• Storage and shipping (item-specific):
  • Storage: Room temperature (per Product Data). Protect from moisture; reseal promptly after opening.
  • Shipped: Normal (per Product Data).

Note: If critical for your application, perform in-house growth promotion testing with your target strains to verify suitability of the lot under your incubation conditions.

Reaction and Applications

This product is a microbiological culture medium, not a reagent for organic or inorganic chemical synthesis. Relevant applications center on microbial cultivation and analysis (general, literature):

• Cultivation/enumeration of lactic acid bacteria and related fastidious organisms, including Lactobacillus, Leuconostoc, Pediococcus, and some Bifidobacterium under appropriate conditions.
• Maintenance of starter cultures and propagation for downstream assays (e.g., carbohydrate fermentation profiles, metabolite production studies, and enzyme activity screens).
• Food, beverage, and fermentation research for monitoring LAB populations in dairy, meat, vegetable fermentations, and beverage matrices.
• Probiotic R&D and raw-material assessments: growth promotion testing and viability checks (non-GMP research contexts).
• Antimicrobial susceptibility or challenge studies against LAB in research settings (using broth microdilution or time–kill formats), recognizing that medium composition affects apparent activity.

Practical tips (general):

• Deaeration/oxygen control: LAB growth can improve under reduced redox potential; consider pre-reducing broth (e.g., by anaerobic incubation or addition of redox agents where appropriate) or incubating in anaerobic jars.
• pH control: LAB generate lactic acid; overlay with sterile mineral oil or use CO2-enriched/incubator conditions as needed; for extended cultures, consider buffering or pH-stat fermentation.
• Inoculum size and incubation temperature should match organism requirements (commonly 25–37 °C; consult strain datasheets).

Reaction Conditions

As a culture medium, “reaction conditions” correspond to preparation and incubation parameters. The following are general, literature-based guidelines for APT-type media and are not item-specific specifications:

• Reconstitution (typical literature formulation):
  • Dissolve the recommended grams per liter (g/L) indicated on the product label or CoA (value not specified for this item) in purified water with gentle heat and stirring.
  • Adjust volume to final mark; do not overheat prior to sterilization.
• Sterilization:
  • Autoclave at 121 °C for 15 minutes (liquids cycle). For heat-labile supplements (e.g., antibiotics), sterile-filter and add after cooling to ~45–50 °C.
• Incubation conditions (organism-dependent):
  • Temperature: commonly 25–37 °C for lactic acid bacteria; select per strain guidance.
  • Atmosphere: aerobic, microaerophilic, or anaerobic as required. Reduced redox potential often benefits LAB; consider anaerobic jars or CO2 incubation.
  • Duration: 18–48 hours for routine growth; extended incubation may be needed for slow growers.
• Performance checks:
  • Conduct growth promotion testing with designated control strains to verify lot suitability and local water/sterilization effects.

Exact g/L dosage, starting pH, and performance specifications are not provided for this SKU; consult the CoA/Spec Sheet for authoritative instructions.

Safety and Handling

• GHS classification, signal word, H/P statements, pictograms: Not specified for this item; refer to the product SDS for authoritative information.
• General laboratory precautions (good practice for dehydrated culture media; literature/general):
  • Avoid creating dust/aerosols; may cause mechanical/respiratory irritation. Use in a well-ventilated area or biological safety cabinet when handling dry powders.
  • Wear appropriate PPE: lab coat, safety glasses, and nitrile gloves. Wash hands after handling.
  • Prevent microbial contamination of the dry medium and solutions; handle reconstituted broth aseptically.
• Incompatibilities (general):
  • Strong oxidizers and acids/alkalis may degrade organic components. Avoid prolonged light and humidity exposure which can lead to clumping or performance drift.
• First-aid (general guidance; defer to SDS/site SOPs):
  • Inhalation: Move to fresh air; seek medical attention if irritation persists.
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing; obtain medical advice if irritation continues.
  • Ingestion: Rinse mouth with water; seek medical attention as needed.
• Microbiological safety:
  • Reconstituted medium can support microbial growth; treat spent media and cultures as biohazardous waste. Autoclave or chemically disinfect before disposal per institutional biosafety procedures.

Solvent Selection

APT Broth is intended to be reconstituted in water; organic solvent selection is not applicable.

• Primary solvent: High-purity water (e.g., ASTM Type 1 or Type 2). Avoid ionic or organic contaminants that may inhibit fastidious organisms.
• Sterilization (general, literature):
  • Autoclaving at 121 °C for ~15 minutes is standard for bulk volumes. For heat-labile supplements (e.g., antibiotics), sterile-filter and add aseptically post-autoclave after cooling to ~45–50 °C.
• Water quality considerations:
  • Elevated TOC, residual chlorine, or heavy metals can suppress growth performance; use validated lab water sources.
• Comparison (general media choices):
  • APT vs. MRS: Both support lactic acid bacteria. APT often includes polysorbate and citrate buffering; MRS is more selective for Lactobacillus spp. Choice depends on target spectrum and desired selectivity.

Item-specific solvent compatibility limits (pH, ionic strength, supplements): Not specified for this item; refer to CoA/Spec Sheet.

Storage and Reconstitution

• Item-specific storage: Room temperature (per Product Data). Keep container tightly closed, dry, and protected from humidity and direct sunlight. Reseal promptly after each use or transfer to a desiccated cabinet.
• Shipping: Normal (per Product Data).
• Reconstitution details (g/L, pH targets, sterilization): Not specified for this item; refer to CoA/Spec Sheet.
• Stability notes (general, literature):
  • Dehydrated medium is generally stable at room temperature if kept dry; avoid caking due to moisture ingress.
  • Prepared (sterile) broth should be stored at 2–8 °C and used within a short period (commonly days to a few weeks depending on container and sterility); verify performance before critical use.
• Freeze–thaw: Not applicable to the dry powder. Avoid freezing prepared broth unless validated, as precipitation or component degradation may occur.

Always consult the lot-specific CoA/SDS for definitive handling instructions and any performance specifications.

Structure and Identity

APT Broth is a complex, undefined nutrient medium formulated for the cultivation of fastidious and fermentative bacteria (not a single chemical substance).

• Item-specific identifiers:
  • CAS: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular formula: Not applicable (complex mixture).
  • Molecular weight: Not applicable (complex mixture).
  • SMILES / InChIKey: Not applicable (complex mixture).
• Structural features (general, literature):
  • Contains protein/peptide hydrolysates and yeast extract (sources of amino acids, peptides, vitamins, nucleotides).
  • Includes carbohydrate(s) as fermentable energy (e.g., dextrose) and nonionic surfactant (polysorbate/Tween) as a fatty acid source and to enhance nutrient availability.
  • Typically buffered with phosphate/citrate salts and supplemented with divalent cations (e.g., Mg2+, Mn2+) to support lactic metabolism and enzyme function.
• 2D “structure” description in words:
  • Heterogeneous blend of organic and inorganic nutrients; no single repeating unit or defined molecular structure. The medium is supplied dehydrated and, upon dissolution in water, yields a balanced broth supporting growth of lactic acid bacteria and other fastidious organisms.

Note: All composition details above are general/literature descriptions of APT-type media and are not item-specific specifications for this SKU.

Synthetic Utility

Not applicable. APT Broth is not a reagent for chemical synthesis and does not participate as a defined reactant or catalyst in named organic or inorganic reactions.

For laboratories integrating chemistry and microbiology, APT Broth may serve as the biological culture stage upstream of chemical analytics (e.g., metabolite profiling by HPLC/GC-MS), but it does not provide synthetic transformations itself.

Target Specificity

Not applicable to this product type. Target specificity (antigen/epitope/clone) pertains to antibodies or affinity reagents, and no such data are provided for this SKU.

Foire aux questions

How should this product be stored?

Store at room temperature.