B-Phycoerythrin (B-PE) - BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥20 mg/ml; A545/A280>5.5; A565/A545>1.05; A620/A545

Cat. No.: B1451318

GRADE & PURITY BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥20 mg/ml; A545/A280>5.5; A565/A545>1.05; A620/A545<0.005

Storage

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing

Shipped In

Wet ice

Application

Cell Staining, Fluorescence Analysis, Protein/Antibody Labeling

Vue d’ensemble

B-Phycoerythrin (B-PE) and the closely related R-Phycoerythrin (R-PE) are the most intensely fluorescent phycobiliproteins. B-PE, considered the world's brightest fluorophore, exhibits significantly bright and photostable orange fluorescence.

B-PE is compatible with commonly available lasers (520 nm) and provides efficient, accurate, less background interference results in Flow Cytometry (FC) and Immunofluorescence microscopy (IF).

Aladdin's B-PE absorbance peaks are at 545 and 563 nm, and its significant stokes shifts with a resolvable emission peak at 578 nm.

Benefits of B-PE

1. Ideal for cell imaging 

- Applied to most fluorescent-based instruments;

- Stable conjugation with Streptavidin, primary and secondary antibodies.

2. Ensure result accuracy

​- Bright Index: Level 5 with high quantum yield;

- Won't penetrate into the cell and cause a false-positive reaction.

​3. Stability

- Minimal fluorescence quenching;

- Non-toxic, less chance of affecting characteristics of the sample, human body, and environment.

Physical & Spectral Properties

Molecular weight: 240 kDa

Form: Liquid

Absorption maximum (nm): 545

Emission maximum (nm): 578

Specifications

Purity & Impurity:

A545/A280>5.5

A565/A545>1.05

A620/A545<0.005

Applied Instruments & Applications

1. Applied to most lab fluorescent-based instruments:

- Flow Cytometry (FC)

- Immunofluorescence Assay (IFA)

2. Can be used in researches such as:

- Microfluidic Biochip

- Circulating Tumor Cell (CTC) Research

- Cell Therapy

- and other laboratory researches

*Note: This reagent is for research use only, not for diagnostic or therapeutic use.

Specifications

Spécifications et pureté

BioReagent, Biological Stain, for Fluorescence analysis, for Microscopy, ≥20 mg/ml; A545/A280>5.5; A565/A545>1.05; A620/A545<0.005

Stabilité et stockage

Store at 2-8℃ long term (24 months). Store in the dark. Desiccated. DO NOT FREEZE.

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Avoid repeated freezing and thawing

Expédié en

Wet ice

Ce produit nécessite l'expédition en chaîne froide. Les services terrestres et autres services économiques ne sont pas disponibles.

Grade

Biological Stain, BioReagent, for Fluorescence analysis, for Microscopy