CD20 Mouse mAb (AF488) - Primary antibody, specific to CD20

Cat. No.: Ab191694

QUALITÉ & PURETÉ ExactAb™, Validated, 5 μL/test

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human, Monkey

Isotype

Mouse IgG2b

Clone Number

2H7

Conjugation

AF488

Vue d’ensemble

Mouse anti Human/Monkey CD20 Antibody, Monoclonal (2H7), could be used for Flow Cytometry.

Spécifications

Product Name

CD20 Mouse mAb (AF488) - Primary antibody, specific to CD20

Synonymes

FMC7|B-lymphocyte antigen CD20|B-lymphocyte cell-surface antigen B1|B-lymphocyte surface antigen B1|Bp35|Bp35MGC3969|CD20 antigen|CD20 receptor|CD20|LEU-16|Leukocyte surface antigen Leu-16|Ly-44|Membrane-spanning 4-domains subfamily A member 1|membrane-spanning 4-domains, subfamily A, member 1|S7|APY antibody|ATOPY antibody|B lymphocyte antigen CD20 antibody|B Lymphocyte Cell Surface Antigen B1 antibody|B-lymphocyte cell-surface antigen B1 antibody|B-lymphocyte surface antigen B1 antibody|B1 antibody|Bp 35 antibody|Bp35 antibody|CD 20 antibody|CD20 antibody|CD20 antigen antibody|CD20 receptor antibody|CVID 5 antibody|CVID5 antibody|Fc epsilon receptor I beta chain antibody|Fc Fragment of IgE high affinity I receptor for beta polypeptide antibody|FCER1B antibody|High affinity immunoglobulin epsilon receptor subunit beta antibody|IgE Fc receptor subunit beta antibody|IGEL antibody|IGER antibody|IGHER antibody|LEU 16 antibody|Leu-16 antibody|LEU16 antibody|leukocyte surface antigen Leu 16 antibody|Leukocyte surface antigen Leu-16 antibody|Ly44 antibody|Membrane spanning 4 domains A1 antibody|Membrane spanning 4 domains subfamily A member 2 antibody|membrane-spanning 4-domains A1 antibody|Membrane-spanning 4-domains subfamily A member 1 antibody|MGC3969 antibody|MS4A1 antibody|MS4A2 antibody|S7 antibody

Spécifications et pureté

ExactAb™, Validated, 5 μL/test

Mécanismes biochimiques et physiologiques

This protein may be involved in the regulation of B-cell activation and proliferation.

Espèce hôte

Mouse

Spécificité

CD20

Immunogène

Human tonsillar B cells

Contrôle positif

Flow: Human peripheral blood lymphocytes

Conjugation

AF488

Excitation(nm)

Blue Laser(488nm)

Émission (nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grade

ExactAb™, Validated

Type de chaîne lumineuse

kappa

Méthode de purification

Protein A purified

Concentration

5 μL/test

Product Line

Produits biologiques

Stockage et expédition

Conditions de stockage de stockage

Store at 2-8°C,Protected from light,Do not freeze

Expédié en

Wet ice,Do not freeze

Stabilité et stockage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD20 Mouse mAb (AF488) (Ab191694)

CD20 Mouse mAb (AF488) (Ab191694) - Flow Cytometry
Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD20 Mouse mAb (AF488) (Ab191694) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

Flow Cytometry: CD20 Mouse mAb (AF488) (Ab191694)

CD20 Mouse mAb (AF488) (Ab191694) - Flow Cytometry
Flow cytometry analysis of CD20 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD20 Mouse mAb (AF488) (Ab191694) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.

Application

Application

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Cibles associées (humaines)

MS4A1 Tclin B-lymphocyte antigen CD20 (0 Activities)

Discover Target: MS4A1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD20 Mouse mAb (AF488)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.