AcM de rat CD8 alpha (AF647) - Primary antibody, specific to CD8 alpha, Rat IgG2a

Cat. No.: Ab190218
DISPONIBLE À COMMANDE
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Ex:650nm ? Fluorescence excitation maximum at 650 nm — the wavelength of light that best excites this fluorophore. Match it to your instrument's laser or filter set for the strongest signal. Em:668nm ? Fluorescence emission maximum at 668 nm — the wavelength this fluorophore emits after excitation. Use it to pick the right detection filter and avoid channel cross-talk. 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2a
Clone Number
53-6.7
Conjugation
AF647
Size
USA
Allemagne (EU)*
Price
Qty
10μg
Ab190218-10μg
4 En stock

37,90$US

47,90$US
Enregistrer 10,00 $US (20.88%)
25μg
Ab190218-25μg
2 En stock

71,90$US

95,90$US
Enregistrer 24,00 $US (25.03%)
100μg
Ab190218-100μg
1 En stock

171,90$US

206,90$US
Enregistrer 35,00 $US (16.92%)
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, validé, Ex:650nm, Em:668nm, 0.5 mg/mL Em:668nm,Ex:650nm,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

A conserver à 2-8°C,A l'abri de la lumière,Interdit au congélateur Ships Glace humide Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Vue d’ensemble

Anticorps monoclonal anti-souris CD8 alpha (53-6.7), pouvant être utilisé pour le Flow, l'ICC/IF, le WB, la déplétion des cellules T CD8+ in vivo, etc.

Specifications

Product Name
AcM de rat CD8 alpha (AF647) - Primary antibody, specific to CD8 alpha, Rat IgG2a
Synonymes
Ly-2 | Ly-35 | Ly-B | Lyt-2 | CD_antigen : CD8a | AF647 Anticorps anti-souris CD8a (53-6.7) | AF647 Anticorps anti-souris CD8a [53-6.7] | Anticorps monoclonal CD8a (53-6.7), AF647 | AF647 Anticorps anti-souris CD8a | Antigène de différenciation des lympho
Spécifications et pureté
ExactAb™, validé, Ex:650nm, Em:668nm, 0.5 mg/mL
Mécanismes biochimiques et physiologiques
Identifie les cellules T cytotoxiques/suppressives qui interagissent avec les cibles porteuses du CMH de classe I. On pense que le CD8 joue un rôle dans le processus de destruction médiée par les cellules T. Les chaînes alpha du CD8 se lient aux domaines
Espèce hôte
Rat
Spécificité
CD8 alpha
Immunogène
Mouse Spleen Cells or Thymocyte Membranes
Contrôle positif
Flow: C57BL/6 mouse splenocytes.
Conjugation
AF647
Excitation(nm)
Red Laser(633nm)
Émission (nm)
668nm
Ex/Em(nm)
Ex:650nm;Em:668nm
Grade
Em:668nm, Ex:650nm, ExactAb™, Validated
Isotype
Rat IgG2a
Type de chaîne lumineuse
kappa
Méthode de purification
Protein G purified
Concentration
0.5 mg/mL
Stockage et expédition
Conditions de stockage de stockage
A conserver à 2-8°C,A l'abri de la lumière,Interdit au congélateur
Expédié en
Glace humide
Stabilité et stockage
La solution d'anticorps doit être conservée non diluée entre 2°C et 8°C, à l'abri d'une exposition prolongée à la lumière. Ne pas congeler.
Images

CD8 alpha Rat mAb (AF647) (Ab190218) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD8 alpha Rat mAb (AF647) (Ab190218) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF647) (Ab190071).

CD8 alpha Rat mAb (AF647) (Ab190218) - Flow Cytometry
Flow cytometry analysis of CD8 alpha expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD8 alpha Rat mAb (AF647) (Ab190218) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (AF647) (Ab190071). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Cibles associées (humaines)
CD8A Tbio T-cell surface glycoprotein CD8 alpha chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateArticle
ZJ24F1012314Certificate of AnalysisMay 20, 2026 Ab190218
ZJ24F1012315Certificate of AnalysisMay 20, 2026 Ab190218
ZJ24F1012316Certificate of AnalysisMay 20, 2026 Ab190218
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide → View Ex:650nm grade guide → View Em:668nm grade guide →

Produits apparentés

Rat IgG - Isotype Control Antibody, IgG
Goat Anti-Rat IgG H&L Antibody - Secondary antibody, Goat IgG
CD8 alpha Rat mAb - Primary antibody, specific to CD8 alpha, Rat IgG2b
Goat Anti-Rat IgG H&L (Biotin) - Secondary antibody, Goat IgG
Rat IgG (Biotin) - Isotype Control Antibody, IgG
Rat IgG (PE) - Isotype Control Antibody, IgG
Rat IgG (FITC) - Isotype Control Antibody, IgG
Rat IgG (APC) - Isotype Control Antibody, IgG
CD8 alpha Rat mAb (APC) - Primary antibody, specific to CD8 alpha, Rat IgG2b
CD8 alpha Rat mAb (PE) - Primary antibody, specific to CD8 alpha, Rat IgG2b
CD8 alpha Rat mAb (FITC) - Primary antibody, specific to CD8 alpha, Rat IgG2b
CD8 alpha Rat mAb (Biotin) - Primary antibody, specific to CD8 alpha, Rat IgG2b
CD8 alpha Rat mAb - Primary antibody, specific to CD8 alpha, Rat IgG2a
Rat IgG2a-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Rat IgG2a
CD8 alpha Rat mAb - Primary antibody, specific to CD8 alpha, Rat IgG2b
Rat IgG2b-kappa, anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD, Rat IgG2b
Rat IgG1-kappa - Isotype Control Antibody, Rat IgG1
Rat IgG (AF405) - Isotype Control Antibody, IgG
Rat IgG (AF647) - Isotype Control Antibody, IgG
IgG de rat (AF700) - Isotype Control Antibody, IgG
AcM de rat CD8 alpha (biotine) - Primary antibody, specific to CD8 alpha, Rat IgG2a
AcM de rat CD8 alpha (AF405) - Primary antibody, specific to CD8 alpha, Rat IgG2a
AcM de rat CD8 alpha (FITC) - Primary antibody, specific to CD8 alpha, Rat IgG2a
CD8 alpha Rat mAb (PE) - Primary antibody, specific to CD8 alpha
Rat IgG (AF555) - Isotype Control Antibody
Rat IgG2b-kappa (Biotin), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2b-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2b-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2b-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2b-kappa (AF647), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2a-kappa (AF405), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2a-kappa (AF647), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2a-kappa (FITC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2a-kappa (APC), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2b-kappa (AF700), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2b-kappa (AF405), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG2a-kappa (AF700), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG (PE-Cy5) - Isotype Control Antibody
Rat IgG (APC-Cy7) - Isotype Control Antibody
Recombinant CD8α Antibody - Primary antibody, specific to Mouse CD8α
Recombinant CD8 alpha Antibody (PE) - Primary antibody, specific to Mouse CD8α
Recombinant CD8 alpha Antibody (Biotin) - Primary antibody, specific to Mouse CD8α
CD8 alpha Rat mAb (PE-Cy5) - Primary antibody, specific to CD8 alpha
CD8 alpha Rat mAb (PE-Cy7) - Primary antibody, specific to CD8 alpha
CD8 alpha Rat mAb (AF488) - Primary antibody, specific to CD8 alpha
Rat IgG2a-kappa (AF555), anti-Spike RBD - Isotype Control Antibody, specific to Spike RBD
Rat IgG (AF488) - Isotype Control Antibody
Rat IgG (PE-Cy7) - Isotype Control Antibody

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