CD86 Rat mAb - Primary antibody, specific to CD86, Rat IgG2a

Cat. No.: Ab095462

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, See COA

Application

Animal Model, Blocking, ELISA, Flow Cytometry, Functional Assay, IHC, IHC-F, IP, WB

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

Unconjugated

Overview

 Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry, WB, IHC-F, IP, Blocking, ELISA, Functional Assay, Animal Model and so on.

Specifications

Product Name

CD86 Rat mAb - Primary antibody, specific to CD86, Rat IgG2a

Synonyms

Activation B7-2 antigen | B70 | B7-2 antigen | B72 | B7-2 | B-lymphocyte activation antigen B7-2 | BU63 | CD28 antigen ligand 2 | CD28LG2B7-2 antigen) | CD86 antigen | CD86 molecule | CD86 | CTLA-4 counter-receptor B7.2 | FUN-1 | LAB72 | MGC34413 | T-lymp

Specifications & Purity

Carrier Free, ExactAb™, Azide Free, Validated, PBS Only, See COA

Biochemical and Physiological Mechanisms

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Host species

Rat

Specificity

CD86

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Positive Control

Flow: Mouse splenocytes.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotype

Rat IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at 2-8°C,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

Store at 2-8°C. Store at -20°C for long term. Upon delivery aliquot. Avoid freeze/thaw cycle.

Images

Flow Cytometry: CD86 Rat mAb (Ab095462)

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 (red) with CD86 Rat mAb (Ab095462) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Isotype control, Rat IgG Isotype Control Antibody (Biotin) (Ab180310). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: CD86 Rat mAb (Ab095462)

CD86 Rat mAb (Ab095462) - Flow Cytometry
Flow Cytometry analysis of stimulated with LPS (L118716) for 24h mouse splenocytes cells labelling CD86 with CD86 Rat mAb (Ab095462) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with Rat IgG Isotype Control Antibody (Biotin) (Ab180310) - Isotype Control (left panel) and detected with SA-PE (rp156150).

Application

• Application: Dilution info
• Flow Cytometry:

  0.25μg/100w cells;

• WB:

  Use at an assay dependent concentration.;

• Blocking:

  Use at an assay dependent concentration.;

• Functional Assay:

  Use at an assay dependent concentration.;

• IP:

  Use at an assay dependent concentration.

• ELISA:

  Use at an assay dependent concentration.;

• Animal Model:

  Use at an assay dependent concentration. ;

• IHC-F:

  Use at an assay dependent concentration.

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action