GRADE & PURITYBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.
BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C Ships Wet ice Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Vue d’ensemble
Plasma total cholesterol consists of two-thirds cholesterol esters and one-third free cholesterol. It is a key component of blood lipids, and both forms are closely associated with cardiovascular and cerebrovascular diseases as well as various pathological conditions. This kit employs the total cholesterol and free cholesterol detection methods recommended by the World Health Organization (WHO), the U.S. Food and Drug Administration (FDA), and China's National Clinical Laboratory Operating Procedures, offering high sensitivity with a detection range of 20–5000 µmol/L, as well as excellent reliability and reproducibility.
The kit utilizes the CHOD-PAP endpoint method combined with the classic GPO Trinder enzymatic reaction. The principle is as follows: (1) Cholesterol esterase hydrolyzes cholesterol esters into free cholesterol; (2) Cholesterol oxidase oxidizes free cholesterol to produce hydrogen peroxide; (3) Under the catalysis of peroxidase, the chromogenic substrate is converted into benzoquinone imine, with the optical density being proportional to the cholesterol concentration; (4) The total cholesterol concentration and free cholesterol concentration are calculated separately, and the difference between the two values represents the cholesterol ester concentration.
Specifications
Spécifications et pureté
BioReagent
Grade
BioReagent
Application
Cell Metabolism
Detection instrument
Microplate reader,Visible Spectrophotometer
Detection wavelength
550nm
Stockage et expédition
Conditions de stockage de stockage
Store at 2-8°C
Expédié en
Wet ice
Stabilité et stockage
Store at 2-8℃ for 6 months.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
