GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.0.5 mg/mL
ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.
Post-translational: Autoubiquitinated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Host species
Mouse
Specificity
CNOT4
Immunogen
Recombinant fusion protein of human CNOT4.
Positive Control
WB: HEK293, Hep G2, HeLa, NIH/3T3 and C6 cell lysates.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
CNOT4 Mouse mAb (Ab097034) - Western Blot All lanes: CNOT4 Mouse mAb (Ab097034) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 56 kDa Exposure time: 40.6 s
Application
Application
Dilution info
WB
1/500-1/4000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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