EB Solution (1 mg/mL) - BioReagent, Biological Stain, for NA electrophoresis, 1 mg/ml in DMF , CAS No.1239-45-8

CAS: 1239-45-8 Cat. No.: E1526367 Formule: C₂₁H₂₀BrN₃ Poids moléculaire: 394.32 Numéro CE: 214-984-6

QUALITÉ & PURETÉ BioReagent, Biological Stain, for NA electrophoresis, 1 mg/ml in DMF

Synonyms

1 mg/ml Ethidium Bromide Aqueous Solution

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining, Nucleic Acid Electrophoresis

Vue d’ensemble

Ethidium Bromide (molecular formula: C₂₁H₂₀BrN₃, molecular weight: 394.31) is a highly sensitive fluorescent dye used for visualizing DNA in agarose and polyacrylamide gels. It emits an orange-red fluorescence signal when excited by a 302 nm UV transilluminator. EB is toxic; all EB-containing solutions must be decontaminated before disposal to prevent environmental pollution. EB Solution (1mg/mL) is prepared with sterile double-distilled water and is widely applied in electrophoresis of DNA and RNA samples.

Required Materials:

1. Electrophoresis running buffer (e.g., TAE, TBE)

2. Microwave oven

3. UV analyzer or gel documentation imaging system

Precautions:

1. Gel thickness should not exceed 0.5 cm; excessive thickness will reduce detection sensitivity.

2. Avoid prolonged UV exposure when recovering DNA fragments via gel electrophoresis.

3. EB is toxic and irritates the skin and eyes. Handle with extreme caution.

4. For personal safety, wear a lab coat, disposable gloves and a face mask during operation.

5. This product is for scientific research use by qualified professionals only. It must not be used for clinical diagnosis or treatment, food or pharmaceutical production, nor stored in residential premises.

Spécifications

Synonymes

1 mg/ml Ethidium Bromide Aqueous Solution

Spécifications et pureté

BioReagent, Biological Stain, for NA electrophoresis, 1 mg/ml in DMF

Stabilité et stockage

Store at room temperature long term (12 months). Store in the dark.

Conditions de stockage de stockage

Protected from light,Room temperature

Expédié en

Normal

Grade

Biological Stain, BioReagent, for NA electrophoresis

Product Line

Produits biologiques

Noms et identifiants

Isomères SMILES

CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]

WGK Allemagne

3

RTECS

SF7950000

Poids moléculaire

394.32

Reaxy-Rn

3642536

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=3642536&ln=

Propriétés chimiques et physiques

• Sensibilité: Light-sensitive
• Point de fusion (°C): 260-262°C