ErbB2/Her2 Mouse mAb - Primary antibody, specific to ErbB2/Her2

Cat. No.: Ab102062

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2a

Conjugation

Unconjugated

Overview

Mouse anti Human ErbB2/Her2 Antibody, Monoclonal (OTI1C5), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

ErbB2/Her2 Mouse mAb - Primary antibody, specific to ErbB2/Her2

Synonyms

Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody | C erb B2/neu protein antibody | CD340 antibody | CD340 antigen antibody | Cerb B2/neu protein antibody | CerbB2 antibody | Erb b2 receptor tyr

Specifications & Purity

Validated, ExactAb™, See COA

Biochemical and Physiological Mechanisms

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a

Host species

Mouse

Specificity

ErbB2/Her2

Immunogen

Recombinant protein of human ERBB2 (AA 676-1255).

Positive Control

WB: SK-OV-3 cell lysate. IHC: Human breast tissue. IF/ICC: COS7 cells. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY ERBB2.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Purification Method

Protein G purified

Concentration

See COA

Storage and Shipping

Shape

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: ErbB2/Her2 Mouse mAb (Ab102062)

ErbB2/Her2 Mouse mAb (Ab102062) - Western Blot
All lanes: ErbB2/Her2 Mouse mAb (Ab102062) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 138 kDa
Observed band size: 179 kDa
Exposure time: 100.0 s

IHC: ErbB2/Her2 Mouse mAb (Ab102062)

ErbB2/Her2 Mouse mAb (Ab102062) - IHC
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using ErbB2/Her2 Mouse mAb (Ab102062). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120 °C for 3 min.

IF/ICC: ErbB2/Her2 Mouse mAb (Ab102062)

ErbB2/Her2 Mouse mAb (Ab102062) - IF/ICC
ErbB2/Her2 Mouse mAb (Ab102062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2.

Flow: ErbB2/Her2 Mouse mAb (Ab102062)

ErbB2/Her2 Mouse mAb (Ab102062) - Flow
HEK293T cells transfected with either pCMV6-ENTRY ERBB2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ErbB2/Her2 Mouse mAb (Ab102062), and then analyzed by flow cytometry.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/150

Associated Targets(Human)

ERBB2 Tclin Receptor tyrosine-protein kinase erbB-2 (0 Activities)

Discover Target: ERBB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)