Dissolve 30.5 grams per liter of distilled/deionized water, heating to boiling with stirring until completely solubilized.
Dispense into appropriate containers, loosen caps and autoclave for 15-30 minutes at 121ºC (15 psi).
Component
g/liter
Pancreatic Digest of Casein
4
Pancreatic Digest of Gelatin
6
Beef Extract
1.5
Yeast Extract
3
Dextrose
1
Agar
15
Total: 30.5g/Liter
Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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