Brain Heart Infusion Agar (Powder)

Directions per Liter:

Dissolve 52 grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Brain Heart Infusion (from solids)	8
Peptic Digest of Animal Tissue	5
Pancreatic Digest of Casein	16
Sodium Chloride	5
Dextrose	2
Disodium Phosphate	2.5
Agar	13.5
	Total: 52g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.