Chapman Stone Medium (Powder)

Directions per Liter:

Dissolve 202 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Component	g/liter
Ammonium Sulfate	75
Sodium Chloride	55
Gelatin	30
Agar	15
D-Mannitol	10
Pancreatic Digest of Casein	10
Potassium Phosphate, Dibasic	5
Yeast Extract	2
	Total: 202g/Liter

Storage and Stability:

Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.