FGFBP1 Mouse mAb: Selection & Application Guide

FGFBP1 Mouse mAb is a monoclonal antibody raised in mouse against human Fibroblast Growth Factor Binding Protein 1 (FGFBP1), supplied in PBS buffer and intended for use in standard immunological research applications.

Product Name

FGFBP1 Mouse mAb

SKU

Ab350078

Host Species

Mouse

Formulation

PBS (Phosphate-Buffered Saline) only

Antibody Type

Monoclonal Antibody (mAb)

Target Protein

FGFBP1 (Fibroblast Growth Factor Binding Protein 1)

Overview of FGFBP1 and Its Biological Significance

Fibroblast Growth Factor Binding Protein 1 (FGFBP1) is a secreted chaperone protein that binds to members of the fibroblast growth factor (FGF) family, facilitating their mobilization from extracellular matrix heparan sulfate proteoglycan storage sites and potentiating FGF signaling. FGFBP1 plays important roles in angiogenesis, wound healing, and tumor progression, making it a target of significant interest in oncology and vascular biology research.

Researchers studying FGF-dependent signaling pathways, tumor microenvironment remodeling, or tissue repair processes frequently require reliable detection tools for FGFBP1. A high-quality monoclonal antibody such as FGFBP1 Mouse mAb (Ab350078) provides the specificity and reproducibility essential for these investigations.

Product Specifications and Formulation Details

FGFBP1 Mouse mAb (Ab350078) is produced in mouse and supplied exclusively in PBS (Phosphate-Buffered Saline) without the addition of glycerol, BSA, or azide preservatives, unless otherwise noted on the specific lot certificate of analysis. Researchers should consult the product datasheet and certificate of analysis for lot-specific information regarding concentration, purity, and validated applications.

Key specifications to verify upon receipt include:

• Host species: Mouse
• Antibody class: Monoclonal (mAb)
• Target: FGFBP1 (human)
• Formulation: PBS only
• SKU: Ab350078

Because the formulation contains PBS only, without stabilizing additives, special attention to aliquoting and storage conditions is strongly advised to maintain antibody activity.

Storage, Handling, and Aliquoting Guidelines

Proper storage and handling are critical for maintaining the performance of FGFBP1 Mouse mAb (Ab350078), particularly because the PBS-only formulation lacks stabilizing additives such as glycerol or BSA.

Recommended storage conditions:

• Store at –20 °C for long-term storage. Avoid repeated freeze–thaw cycles, as these can denature the antibody and reduce binding activity.
• For short-term use (up to 1–2 weeks), the antibody may be stored at 4 °C.
• Upon first use, prepare single-use aliquots to minimize freeze–thaw cycling.

Handling precautions:

• Briefly centrifuge the vial before opening to collect all liquid at the bottom.
• Work on ice when handling the antibody solution to minimize activity loss.
• Avoid prolonged exposure to light if the antibody is conjugated to a fluorophore; for the unconjugated form, standard laboratory light conditions are acceptable.
• Use low-protein-binding tubes for aliquoting to prevent adsorptive losses.

Always refer to the lot-specific certificate of analysis for confirmed storage recommendations.

Recommended Applications and Protocol Guidance

FGFBP1 Mouse mAb (Ab350078) is intended for use in immunological research applications. While specific validated applications should be confirmed on the product datasheet, mouse monoclonal antibodies targeting secreted extracellular proteins such as FGFBP1 are commonly evaluated in the following assays:

• Western Blot (WB): Detect FGFBP1 in cell lysates or conditioned media. Recommended to use reducing SDS-PAGE conditions and optimize primary antibody concentration empirically.
• Enzyme-Linked Immunosorbent Assay (ELISA): Suitable for quantitative detection of secreted FGFBP1 in conditioned medium or serum samples when used as a capture or detection antibody.
• Immunofluorescence (IF) / Immunohistochemistry (IHC): For localization studies in cell lines or tissue sections; antigen retrieval optimization may be required for FFPE tissue sections.
• Immunoprecipitation (IP): May be used to pull down FGFBP1-FGF complexes for co-immunoprecipitation studies of binding partners.

General protocol tips:

• Optimize primary antibody dilution for each application and sample type using a dilution series.
• Include appropriate positive controls (e.g., cell lines known to express FGFBP1) and negative controls (e.g., isotype control antibody).
• Use a secondary antibody matched to the mouse IgG Fc region for detection in indirect immunoassay formats.

Always confirm validated applications and recommended dilutions on the current lot-specific datasheet before proceeding.

Quality Control and Troubleshooting

To ensure reliable results with FGFBP1 Mouse mAb (Ab350078), implementing a structured quality control workflow is essential.

Quality control checks:

• Confirm antibody integrity upon receipt by checking the vial for signs of precipitation, turbidity, or contamination.
• Record the lot number and verify the certificate of analysis matches the received product.
• Run a pilot experiment with a known FGFBP1-expressing positive control sample before committing to large-scale studies.

Common troubleshooting scenarios:

• No signal / weak signal: Increase primary antibody concentration; verify target expression in the chosen cell or tissue model; confirm secondary antibody compatibility with mouse IgG.
• High background: Reduce primary antibody concentration; increase blocking stringency (e.g., extend blocking time or use alternative blocking reagent); optimize wash steps.
• Non-specific bands (WB): Confirm reducing vs. non-reducing gel conditions; verify sample preparation; try increasing wash buffer stringency with added Tween-20.
• Loss of activity after storage: Check freeze–thaw history; ensure aliquots were prepared; confirm storage temperature was maintained at –20 °C or below.

Contact Aladdin Scientific technical support if troubleshooting does not resolve the issue, providing the lot number and experimental details for expert assistance.

Frequently asked questions

What formulation is FGFBP1 Mouse mAb (Ab350078) supplied in?

FGFBP1 Mouse mAb (Ab350078) is supplied in PBS (Phosphate-Buffered Saline) only, without additional stabilizers such as glycerol or BSA. Researchers should prepare aliquots upon first use and store at –20 °C for long-term preservation.

What is the biological role of FGFBP1 and why is it relevant to research?

FGFBP1 (Fibroblast Growth Factor Binding Protein 1) is a secreted protein that enhances FGF signaling by releasing FGFs from extracellular matrix stores. It is implicated in angiogenesis, tumor development, and wound healing, making it a target of interest in cancer biology and vascular research.

How should I avoid losing antibody activity during repeated experiments?

To preserve activity, aliquot the antibody into single-use volumes immediately upon first thaw and store unused aliquots at –20 °C. Avoid repeated freeze–thaw cycles, keep the antibody on ice during use, and use low-protein-binding tubes to minimize adsorptive losses.

Which secondary antibody should I use with FGFBP1 Mouse mAb?

Since FGFBP1 Mouse mAb is a mouse-derived monoclonal antibody, you should use an anti-mouse IgG secondary antibody appropriate for your detection method (e.g., HRP-conjugated for WB/ELISA, or fluorophore-conjugated for IF/IHC). Confirm the secondary antibody host species and isotype compatibility before use.

Are specific validated applications listed for Ab350078?

Validated applications for Ab350078 should be confirmed on the current product datasheet and lot-specific certificate of analysis available from Aladdin Scientific. Please contact Aladdin Scientific customer support or visit the product page for the most up-to-date application validation information.

References

1. Aladdin Scientific. FGFBP1 Mouse mAb (Ab350078) Product Datasheet. Aladdin Scientific.
2. Tassi E, Wellstein A. The angiogenic switch molecule, secreted FGF-binding protein, an indicator of early stages of pancreatic and colorectal adenocarcinoma. Semin Oncol. 2006;33(1 Suppl 2):S50-6.
3. Wu DQ, Kan MK, Sato GH, Okamoto T, Sato JD. Characterization and molecular cloning of a putative binding protein for heparin-binding growth factors. J Biol Chem. 1991;266(25):16778-85.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.