I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
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I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
·Reviewed·Published ·Updated ·8 min read
I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?
The amount of antibody used is mainly related to the volume of fluid and the number of cells. If the amount of cells is small, the whole system can be halved, that is, 50
μl
cell suspension + 2.5
μl
flow-through antibody. However, if there are very few target cells in the sample, a reduction in the amount of cells may have an effect on the results, resulting in only a small signal being detected.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
Cite this article
Aladdin Scientific. "I only have 2.5×10^5 cells in my cell sample, do I only need to use 1.25 μL of flow-through antibody?" Aladdin Knowledge Base, updated Sep 13, 2024. https://www.aladdinsci.com/us_en/faqs/flow-through-antibody-en.html
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