Hybridization and gene localization experiments in Escherichia coli
Laboratory Protocols & SOPs Library
Hybridization and gene localization experiments in Escherichia coli
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Summary
The E. coli chromosome is ring-shaped. High-frequency recombinant strains (Hfr) have the F factor integrated into their chromosomes, and the chromosomes of the Hfr cells (donor bacteria) are transferred from the Hfr cells into the F- cells when the Hfr bacteria undergo splicing (i.e., hybridization) with the F- bacterial cells.
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Aladdin Scientific. "Hybridization and gene localization experiments in Escherichia coli" Aladdin Knowledge Base, updated 11 nov. 2026. https://www.aladdinsci.com/us_fr/faqs/hybridization-and-gene-localization-expe-en.html
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