Laboratory Protocols & SOPs Library

Modified RIPA Buffer

Recipe

  • 10 mM Tris, pH 7.4

  • 100 mM NaCl

  • 1 mM EDTA

  • 1 mM EGTA

  • 1 mM NaF

  • 20 mM Na4P2O7

  • 2 mM Na3VO4

  • 1% Triton X-100

  • 10% glycerol

  • 0.1% SDS

  • 0.5% deoxycholate

  • plus protease inhibitors: Aprotinin, leupeptin, pepstatin: 1ug/ml each

  • Add 1mM PMSF immediately before use.

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Modified RIPA Buffer" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/us_fr/faqs/modified-ripa-buffer-en.html
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