Nitrobenzene Standard: FAQs for Lab Use & Procurement

Nitrobenzene Standard is a precisely characterized reference material used in analytical chemistry for instrument calibration, method validation, and quantitative determination of nitrobenzene in environmental, industrial, and research samples.

Product Name

Nitrobenzene Standard

SKU

N116219

CAS Number

98-95-3

Molecular Formula

C₆H₅NO₂

Molecular Weight

123.11 g/mol

Primary Application

Analytical calibration and method validation

Typical Storage Condition

Cool, dry, well-ventilated area away from ignition sources

What Is Nitrobenzene Standard and What Is It Used For?

Nitrobenzene Standard (SKU N116219) is a certified reference material containing nitrobenzene (CAS 98-95-3), a pale yellow, oily aromatic compound with the molecular formula C₆H₅NO₂ and a molecular weight of 123.11 g/mol. As a standard, it is supplied at a defined concentration or purity level to enable reproducible analytical measurements.

Primary uses include: instrument calibration for gas chromatography (GC) and high-performance liquid chromatography (HPLC); method validation and performance verification in environmental monitoring laboratories; quantitative analysis of nitrobenzene in water, soil, and industrial effluent samples; and as a retention-time reference or surrogate standard in multi-analyte panels targeting nitroaromatic compounds.

Regulatory agencies including the US EPA list nitrobenzene as a priority pollutant, making certified standards essential for compliance testing in environmental and occupational health contexts.

Purity, Specifications, and Quality Assurance

As an analytical standard, Nitrobenzene Standard N116219 is intended to meet stringent purity requirements. Analytical-grade reference standards for chromatographic use are typically certified at ≥99% purity, with each lot accompanied by a Certificate of Analysis (CoA) documenting the exact purity value, analytical method used for certification, and traceability information.

When evaluating a standard for regulatory or method-validation purposes, researchers should confirm: (1) the stated purity and its uncertainty; (2) the analytical technique used for purity determination (e.g., GC-FID, GC-MS, or quantitative NMR); (3) lot-specific CoA availability; and (4) traceability to a recognized national metrology institute (NMI) where applicable.

Contact Aladdin Scientific customer support or refer to the product page for lot-specific CoA data for N116219.

Safe Handling, Storage, and Disposal

Nitrobenzene is classified as a toxic substance and suspected human carcinogen. It is readily absorbed through the skin and can cause methemoglobinemia, a condition reducing the blood's oxygen-carrying capacity. All work with nitrobenzene standards must be performed in a well-ventilated fume hood with appropriate personal protective equipment (PPE): chemical-resistant gloves (nitrile or neoprene), lab coat, and safety goggles.

Storage: Keep in a tightly sealed container in a cool (typically 2–8 °C or as indicated on the label), dry location away from heat, sparks, and open flames. Store separately from strong oxidizers and reducing agents. Consult the Safety Data Sheet (SDS) for N116219 prior to use.

Disposal: Nitrobenzene-containing waste must be treated as hazardous chemical waste. Do not dispose of down the drain. Collect in a clearly labelled, solvent-compatible waste container and arrange disposal through a licensed hazardous waste contractor in compliance with local and national regulations.

Always consult the product SDS before handling.

Ordering, Packaging, and Delivery

Nitrobenzene Standard (N116219) is available for order through the Aladdin Scientific website or by contacting the sales team directly. When placing an order, researchers should specify the required pack size and confirm current stock availability, as reference standards may be subject to limited batch quantities.

Packaging is designed to maintain product integrity during transport: amber glass vials or sealed ampoules are typical for liquid standards to minimise light exposure and evaporation. Each shipment includes a CoA and SDS.

Shipping of nitrobenzene-containing products is subject to hazardous materials (HAZMAT) regulations. Shipments are classified under applicable dangerous goods codes (e.g., IATA for air freight or IMDG for sea freight). Delivery timelines vary by destination and shipping mode; confirm lead times with Aladdin Scientific at the time of order.

For bulk quantities, custom concentrations, or special documentation requirements (e.g., for import permits), contact Aladdin Scientific customer support.

Method Integration and Troubleshooting Tips

When integrating Nitrobenzene Standard into an analytical workflow, consider the following best practices:

• Solvent compatibility: Nitrobenzene is miscible with most organic solvents (e.g., methanol, acetonitrile, acetone) but has limited water solubility (~0.19 g/100 mL at 20 °C). Select a diluent compatible with both the standard and the sample matrix.
• Calibration linearity: Prepare a minimum of five calibration levels bracketing the expected sample concentration range. Verify linearity with an R² ≥ 0.999 for quantitative GC or HPLC methods.
• System suitability: Run a system suitability check before each analytical sequence. For GC methods, monitor retention time reproducibility (RSD ≤ 1%) and peak symmetry.
• Stability: Once opened, re-seal immediately and store as directed. Avoid repeated freeze-thaw cycles if the standard is supplied in solution. Verify stability by running a known-concentration check standard periodically.
• Contamination control: Use dedicated, solvent-rinsed glassware to prevent cross-contamination with other nitroaromatic analytes.

If unexpected peaks or retention-time shifts are observed, check column condition, carrier gas purity, and standard lot integrity before re-analysis.

Frequently asked questions

What is the CAS number and molecular formula of Nitrobenzene Standard N116219?

Nitrobenzene Standard N116219 is based on nitrobenzene with CAS number 98-95-3, molecular formula C₆H₅NO₂, and molecular weight 123.11 g/mol. It is a pale yellow, oily liquid with a characteristic almond-like odour.

What purity level can I expect from Nitrobenzene Standard N116219?

Analytical reference standards from Aladdin Scientific are designed to meet high-purity requirements typical for chromatographic calibration use. A lot-specific Certificate of Analysis (CoA) documents the exact purity and the analytical method used for its determination. Please request or download the CoA for your specific lot from the Aladdin Scientific product page or customer support.

Is Nitrobenzene Standard classified as a hazardous material for shipping?

Yes, nitrobenzene is a toxic, flammable liquid classified as a hazardous material under international transport regulations (e.g., IATA for air, IMDG for sea, ADR for road). Shipments of N116219 are prepared and labelled in compliance with applicable dangerous goods regulations. Delivery availability to your location may depend on carrier restrictions; contact Aladdin Scientific for region-specific shipping information.

How should I store Nitrobenzene Standard to maintain its integrity?

Store Nitrobenzene Standard in a tightly sealed, original container in a cool, dry, and well-ventilated area away from heat sources, open flames, and strong oxidisers. Refrigeration (2–8 °C) is commonly recommended for solution standards. Always consult the SDS and CoA label for lot-specific storage instructions.

Can Nitrobenzene Standard N116219 be used for EPA Method 625 or similar environmental methods?

Nitrobenzene is listed as a target analyte in several US EPA methods for semi-volatile organic compound analysis, including EPA Method 625 (base/neutral/acid extractables). To use N116219 for regulatory compliance testing, verify that the lot purity, traceability, and documentation meet your specific method and accreditation requirements. Contact Aladdin Scientific for detailed specification and traceability documentation.

What solvents are compatible with Nitrobenzene Standard for dilution or matrix preparation?

Nitrobenzene is miscible with common organic solvents including methanol, acetonitrile, acetone, dichloromethane, and hexane. Its water solubility is limited (approximately 0.19 g/100 mL at 20 °C). Choose a diluent that matches your analytical method's mobile phase or extraction solvent to ensure accurate calibration and avoid matrix effects.

References

1. US EPA Method 625: Base/Neutrals and Acids. US Environmental Protection Agency, Office of Water.
2. Nitrobenzene Safety Data Sheet. Aladdin Scientific, Product N116219.
3. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Nitrobenzene. International Agency for Research on Cancer.
4. IATA Dangerous Goods Regulations. International Air Transport Association, current edition.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.