Laboratory Protocols & SOPs Library

Phosphate Buffered Saline Tween-20 (PBST) 1x

Recipe

1.Dissolve the following in 800 ml of distilled H2O

  • 8g of NaCl

  • 0.2g of KCl 

  • 1.44g of Na2HPO4

  • 0.24g of KH2PO4

  • 2ml of tween-20

2.Adjust pH to 7.2

3.Adjust volume to 1L with additional distilled H2O

4.Sterilize by autoclaving

Categories: Laboratory Protocols & SOPs Library

Da — when not otherwise indicated, molecular weight units are daltons.   Mw — weight-average molecular weight.   Mn — number-average molecular weight.

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Cite this article

Aladdin Scientific. "Phosphate Buffered Saline Tween-20 (PBST) 1x" Aladdin Knowledge Base, updated 9 juin 2025. https://www.aladdinsci.com/us_fr/faqs/phosphate-buffered-saline-tween-20-pbst-1x-en.html
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