Recombinant Human ATXN7L2 Protein: Key FAQs

Recombinant Human ATXN7L2 Protein is a research-grade recombinant protein corresponding to the human Ataxin-7-like protein 2, a component associated with chromatin-modifying complexes, offered by Aladdin Scientific to support studies in gene regulation, epigenetics, and neurological disease research.

Product Name

Recombinant Human ATXN7L2 Protein

SKU

rp374762

Protein Origin

Human (Homo sapiens)

Protein Family

Ataxin-7-like family; associated with SAGA-type chromatin-modifying complexes

Primary Research Areas

Epigenetics, chromatin remodeling, gene transcription regulation, neurological disease

Supplier Reference

absea#PP-8526

What Is ATXN7L2 and Why Is It Relevant to Research?

ATXN7L2 (Ataxin-7-like protein 2) is a member of the ataxin-7-like protein family. Members of this family are known to participate in large multiprotein chromatin-modifying complexes, most notably the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex and related assemblies. These complexes play critical roles in histone modification, transcriptional co-activation, and the regulation of gene expression programs essential for normal cellular function.

Research interest in ATXN7L2 is driven by its potential roles in epigenetic regulation and its connection to the broader ataxin protein family, which is implicated in neurodegenerative conditions such as spinocerebellar ataxia. Understanding the biochemical function of ATXN7L2 may offer insights into disease mechanisms and novel therapeutic targets.

Product Specifications and Quality Attributes

Recombinant Human ATXN7L2 Protein (SKU: rp374762) is produced as a recombinant protein intended for research use. Key quality attributes researchers should confirm with Aladdin Scientific before ordering include:

• Expression System: Please contact Aladdin Scientific or consult the product datasheet to confirm the specific expression host (e.g., E. coli, baculovirus, or mammalian system), as this affects post-translational modifications and functional assay suitability.
• Purity: Recombinant proteins of this class are typically supplied at >85% purity as assessed by SDS-PAGE; confirm exact purity specification on the current datasheet.
• Tag Information: Many recombinant proteins carry affinity tags (His-tag, GST-tag, etc.) to facilitate purification. Verify tag presence and position in the product datasheet, as tags may affect certain binding or functional assays.
• Formulation and Buffer: Consult the datasheet for the supplied buffer composition, additives (e.g., glycerol), and protein concentration to plan experiments appropriately.

Note: Specific numerical values for purity, concentration, and molecular weight should be verified against the official Aladdin Scientific product datasheet for rp374762.

Storage, Handling, and Reconstitution Guidelines

Proper storage and handling of recombinant proteins are critical to maintaining activity and integrity. General best-practice guidelines applicable to Recombinant Human ATXN7L2 Protein include:

• Storage Temperature: Recombinant proteins are generally stored at -80°C for long-term preservation. Avoid repeated freeze-thaw cycles, which can lead to protein aggregation and loss of activity. Aliquot upon receipt if repeated use is anticipated.
• Short-Term Storage: If the protein will be used within a few days of receipt, storage at 4°C may be acceptable; however, always follow the specific instructions on the product datasheet.
• Reconstitution: If supplied in lyophilized form, reconstitute using the buffer specified in the datasheet (typically sterile PBS or a similar physiological buffer). Allow the vial to equilibrate to room temperature before opening, and gently pipette to mix — avoid vortexing.
• Carrier Protein: For low-concentration working solutions, addition of a carrier protein (e.g., 0.1% BSA) may help prevent adsorption to tube surfaces and maintain stability.

Always refer to the Aladdin Scientific product datasheet for rp374762 for definitive storage and reconstitution instructions.

Research Applications of Recombinant Human ATXN7L2 Protein

Recombinant Human ATXN7L2 Protein is intended for in vitro research applications. Potential uses include, but may not be limited to:

• Protein–Protein Interaction Studies: As a component associated with chromatin-modifying complexes, ATXN7L2 may be used in pull-down assays, co-immunoprecipitation controls, or surface plasmon resonance experiments to characterize binding partners within the SAGA complex or related assemblies.
• Antibody Validation: Recombinant ATXN7L2 protein can serve as a positive control or standard for validating anti-ATXN7L2 antibodies in Western blotting, ELISA, or immunofluorescence workflows.
• Biochemical and Enzymatic Assays: Researchers investigating the functional role of ATXN7L2 in histone modification or transcriptional regulation may use this protein as a substrate or component in in vitro reconstitution experiments.
• Structural Studies: The recombinant protein may be suitable as a starting material for structural biology approaches such as X-ray crystallography or cryo-electron microscopy, subject to purity and formulation requirements.

Suitability for specific applications should be confirmed with Aladdin Scientific technical support prior to experimental design.

Ordering, Pricing, and Technical Support

Recombinant Human ATXN7L2 Protein is available from Aladdin Scientific under SKU rp374762. To place an order or request a quotation, researchers and procurement staff can:

• Visit the Aladdin Scientific website and search by SKU or product name.
• Contact the Aladdin Scientific sales team directly for bulk pricing, custom formulations, or institutional purchase orders.
• Request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) through the product page or customer service.

For technical questions regarding experimental suitability, reconstitution troubleshooting, or application-specific guidance, the Aladdin Scientific technical support team is available to assist. Please have the SKU (rp374762) and your specific application details ready when contacting support to ensure the most accurate and efficient assistance.

Pricing and availability are subject to change; please confirm current details with Aladdin Scientific at the time of ordering.

Frequently asked questions

What is the recommended storage condition for Recombinant Human ATXN7L2 Protein?

For long-term storage, Recombinant Human ATXN7L2 Protein should generally be kept at -80°C, and repeated freeze-thaw cycles should be avoided to preserve activity and integrity. It is advisable to aliquot the protein into single-use volumes upon receipt. Always verify the exact storage conditions specified on the Aladdin Scientific product datasheet for rp374762.

What research applications is Recombinant Human ATXN7L2 Protein validated for?

Recombinant Human ATXN7L2 Protein is intended for in vitro research use and may be applied in protein–protein interaction studies, antibody validation (e.g., as a Western blot positive control), biochemical reconstitution assays, and potentially structural biology investigations. Researchers should confirm the validated applications for rp374762 with Aladdin Scientific technical support before experimental use, as suitability may vary by application and lot.

How should I reconstitute Recombinant Human ATXN7L2 Protein if it is supplied in lyophilized form?

If supplied in lyophilized form, allow the vial to equilibrate to room temperature before opening to prevent condensation. Reconstitute using the buffer recommended in the Aladdin Scientific product datasheet for rp374762 — typically sterile PBS or a similar physiological buffer. Gently pipette to mix without vortexing, and centrifuge briefly to collect any undissolved material. For dilute working solutions, consider adding 0.1% BSA as a carrier to minimize adsorption losses.

What is the biological function of ATXN7L2?

ATXN7L2 (Ataxin-7-like protein 2) is a member of the ataxin-7-like protein family and is associated with SAGA-type chromatin-modifying complexes involved in histone modification and transcriptional co-activation. Its precise molecular function in this context is an active area of investigation. Researchers interested in ATXN7L2 biology should consult current literature, as our understanding of its specific roles continues to evolve.

How do I obtain a Certificate of Analysis or Safety Data Sheet for rp374762?

A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) for Recombinant Human ATXN7L2 Protein (rp374762) can be requested through the Aladdin Scientific product page on the website or by contacting Aladdin Scientific customer service directly. Please have the lot number available when requesting a CoA to ensure you receive the documentation specific to your product lot.

References

1. Helmlinger D, Tora L. Sharing the SAGA. Trends in Biochemical Sciences. 2017;42(11):850-861. [Review of SAGA complex composition and function — ATXN7L2 role should be verified in current literature.]
2. Aladdin Scientific product listing for Recombinant Human ATXN7L2 Protein, SKU rp374762. Reference: absea#PP-8526. [Primary source for product-specific specifications; datasheet should be consulted for current details.]

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.