Recombinant Human C7orf57 Protein: Handling & Usage Protocol

Recombinant Human C7orf57 Protein is a research-grade recombinant protein derived from the human chromosome 7 open reading frame 57 gene, requiring careful reconstitution, aliquoting, and storage procedures to maintain full biological activity for use in functional, binding, and biochemical assays.

Product Name

Recombinant Human C7orf57 Protein

SKU

rp375811

Source Gene

C7orf57 (Chromosome 7 Open Reading Frame 57), Human

Protein Type

Recombinant protein, research grade

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles

Typical Applications

Biochemical assays, protein-protein interaction studies, antibody validation

Materials Required

Before beginning this protocol, ensure the following materials are available in your laboratory:

• Recombinant Human C7orf57 Protein (SKU: rp375811, Aladdin Scientific)
• Sterile ultrapure water or the manufacturer-recommended reconstitution buffer (e.g., PBS, pH 7.4)
• Microcentrifuge tubes (low-protein-binding, 0.5 mL or 1.5 mL)
• Calibrated micropipettes and sterile tips
• Refrigerated microcentrifuge
• Dry ice or liquid nitrogen for snap-freezing aliquots
• –20°C and/or –80°C freezer for long-term storage
• Protein quantification reagents (e.g., BCA or Bradford assay kit)
• Appropriate assay buffers compatible with downstream applications

Consult the product datasheet supplied with SKU rp375811 for lot-specific formulation details, including excipients and lyophilization status, before proceeding.

Reconstitution Procedure

Proper reconstitution is critical for preserving the activity and integrity of Recombinant Human C7orf57 Protein. Follow the steps below carefully:

1. Equilibrate the vial: Allow the lyophilized protein vial to equilibrate to room temperature (18–25°C) for 10–15 minutes before opening. This prevents condensation from entering the vial and reduces osmotic shock to the protein.
2. Centrifuge the vial: Briefly centrifuge the sealed vial at low speed (approximately 500–1000 × g for 30 seconds) to collect any lyophilized material from the sides and cap of the vial.
3. Add reconstitution buffer: Using a sterile micropipette, slowly add the recommended volume of sterile ultrapure water or PBS (pH 7.4) as indicated on the product datasheet for SKU rp375811. Add the buffer gently along the inner wall of the vial to avoid foaming.
4. Dissolve the protein: Gently swirl or pipette the solution up and down. Do NOT vortex, as vigorous mixing can cause protein denaturation and aggregation. Allow the protein to dissolve for 5–10 minutes at room temperature if needed.
5. Verify dissolution: The solution should appear clear or slightly opalescent. Visible particulates may indicate incomplete dissolution or aggregation; if observed, centrifuge at 10,000 × g for 5 minutes at 4°C and use the supernatant.
6. Determine protein concentration: Quantify the reconstituted protein using a BCA or Bradford assay prior to use to confirm the expected concentration stated on the datasheet.

Note: Refer to the lot-specific datasheet for the exact recommended reconstitution volume and buffer composition, as these may vary between lots.

Storage and Stability Guidelines

Correct storage conditions are essential to maintain the activity and stability of Recombinant Human C7orf57 Protein over time. Adhere to the following guidelines:

• Short-term storage (up to 1 week): Store reconstituted protein at 4°C if it will be used within 5–7 days. Addition of a carrier protein such as 0.1% BSA (bovine serum albumin) may help stabilize the protein during short-term refrigerated storage.
• Long-term storage: For storage beyond one week, aliquot the reconstituted protein into single-use volumes using low-protein-binding microcentrifuge tubes and snap-freeze in liquid nitrogen or dry ice. Store aliquots at –80°C for optimal long-term stability.
• Avoid repeated freeze-thaw cycles: Each freeze-thaw cycle can result in partial protein denaturation, aggregation, and loss of biological activity. Prepare single-use aliquots to minimize this risk.
• Lyophilized protein storage: Store the unopened lyophilized vial at –20°C or –80°C, protected from moisture and light, until use.
• Expiry and quality monitoring: Observe the expiration date stated on the product label. Monitor stored aliquots periodically by SDS-PAGE or functional assay to confirm ongoing integrity.

Note: Specific stability data for SKU rp375811 should be obtained from the product datasheet or by contacting Aladdin Scientific technical support.

Application Guidelines and Quality Control

Recombinant Human C7orf57 Protein (rp375811) is intended for research use only. The following guidance supports reproducible results across common applications:

Recommended Applications

• Biochemical assays: Use the reconstituted protein as a substrate, competitor, or positive control in enzyme-linked or bead-based assays. Optimize protein concentration empirically for each assay format.
• Protein-protein interaction studies: Suitable for co-immunoprecipitation (co-IP), pull-down assays, and surface plasmon resonance (SPR) experiments to characterize binding partners of C7orf57.
• Antibody validation: Use as a positive control antigen in Western blot, ELISA, or immunofluorescence assays when validating anti-C7orf57 antibodies.
• Structural studies: May be used as a reference standard in mass spectrometry-based proteomics workflows.

Quality Control Checkpoints

• Confirm protein identity and apparent molecular weight by reducing SDS-PAGE followed by Coomassie staining or silver staining.
• Verify purity (typically ≥85–90% for research-grade recombinant proteins) by densitometry analysis of SDS-PAGE gel bands.
• Assess protein integrity by Western blot using a validated anti-C7orf57 antibody.
• Perform a functional activity assay relevant to your experimental system where applicable.

All quality control data specific to lot rp375811 are available on the accompanying certificate of analysis (CoA). Contact Aladdin Scientific for lot-specific CoA documentation.

Frequently asked questions

What is the recommended reconstitution buffer for Recombinant Human C7orf57 Protein (rp375811)?

The general recommendation is to reconstitute lyophilized recombinant proteins in sterile ultrapure water or PBS (pH 7.4). However, the exact reconstitution buffer and volume for SKU rp375811 should be confirmed by consulting the lot-specific product datasheet supplied with the vial or by contacting Aladdin Scientific technical support, as formulations can vary between lots.

How should I store Recombinant Human C7orf57 Protein to preserve its activity?

Store the lyophilized protein at –20°C or –80°C in a dry, light-protected environment until reconstitution. After reconstitution, aliquot the protein into single-use volumes and store at –80°C for long-term use, or at 4°C for up to one week. Avoid repeated freeze-thaw cycles, which can significantly reduce protein activity and cause aggregation.

What applications is Recombinant Human C7orf57 Protein suitable for?

Recombinant Human C7orf57 Protein (rp375811) is suitable for use in biochemical assays, protein-protein interaction studies (such as co-IP and pull-down assays), antibody validation by Western blot or ELISA, and as a reference standard in proteomics workflows. Optimal working concentrations should be determined empirically for each specific assay format.

How can I confirm the quality and purity of the protein upon receipt?

Upon receipt, review the Certificate of Analysis (CoA) provided with the shipment, which contains lot-specific purity, concentration, and quality control data. You can additionally verify protein identity and purity by running a reducing SDS-PAGE gel followed by Coomassie or silver staining, and confirm identity by Western blot with a validated anti-C7orf57 antibody.

References

1. Aladdin Scientific. Recombinant Human C7orf57 Protein (rp375811) Product Datasheet. Aladdin Scientific; [date unknown].
2. Wingfield PT. Overview of the purification of recombinant proteins. Curr Protoc Protein Sci. 2015;80:6.1.1-6.1.35. doi:10.1002/0471140864.ps0601s80
3. Rosano GL, Ceccarelli EA. Recombinant protein expression in Escherichia coli: advances and challenges. Front Microbiol. 2014;5:172. doi:10.3389/fmicb.2014.00172

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.