Recombinant Human CABCOCO1 Protein: Research Guide

Recombinant Human CABCOCO1 Protein is a research-grade recombinant protein derived from the human CABCOCO1 gene, intended for use in biochemical, cell biological, and structural studies requiring a well-characterized coiled-coil and coenzyme Q biosynthesis domain-containing protein.

Product Name

Recombinant Human CABCOCO1 Protein

SKU

rp365597

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant, coiled-coil domain-containing protein involved in coenzyme Q biosynthesis

Supplier

Aladdin Scientific

Intended Use

Research use only (RUO)

Recommended Storage

-20°C or -80°C; avoid repeated freeze-thaw cycles

Product Overview and Background

Recombinant Human CABCOCO1 Protein (SKU: rp365597) is produced by Aladdin Scientific and is intended exclusively for research purposes. CABCOCO1 (Coiled-coil and Coenzyme Q Biosynthesis Domain Containing Protein 1) is a human protein encoded by the CABCOCO1 gene. As its name suggests, the protein features both coiled-coil structural motifs and a domain associated with coenzyme Q (CoQ, ubiquinone) biosynthesis, linking it to mitochondrial function and cellular energy metabolism.

Coenzyme Q biosynthesis is a highly conserved pathway across eukaryotes, and proteins harboring CoQ biosynthesis-related domains are subjects of increasing research interest in the context of mitochondrial disease, metabolic disorders, and aging. The coiled-coil domain further suggests potential roles in protein–protein interactions and subcellular localization. CABCOCO1 represents an emerging target for researchers investigating mitochondrial biology and related pathologies.

This recombinant protein provides researchers with a quality-controlled reagent for in vitro studies, including binding assays, structural characterization, antibody validation, and functional investigations.

Reconstitution and Handling Guidelines

Proper reconstitution and handling are critical to preserving the activity and integrity of Recombinant Human CABCOCO1 Protein. Follow the guidelines below to ensure optimal performance:

• Reconstitution: Allow the lyophilized protein to equilibrate to room temperature before opening. Reconstitute in the buffer recommended on the product datasheet (typically sterile PBS or a manufacturer-specified buffer) to the indicated concentration. Gently pipette to mix; avoid vortexing, which can denature the protein.
• Centrifugation: Briefly centrifuge the vial before opening to collect any material from the cap or walls.
• Aliquoting: Prepare single-use aliquots immediately after reconstitution to minimize the number of freeze-thaw cycles.
• Carrier Protein: If the protein will be stored at low concentrations, consider adding a carrier protein such as BSA (0.1%) to prevent adsorption to tube surfaces and improve stability.
• Avoid Repeated Freeze-Thaw: Repeated freeze-thaw cycles significantly reduce protein activity. Use each aliquot only once.

Note: Always refer to the current product datasheet (absea#PP-7948) for the most up-to-date reconstitution volumes and buffer compositions, as formulations may vary by lot.

Storage Conditions and Stability

Correct storage is essential for maintaining the quality of Recombinant Human CABCOCO1 Protein over time.

• Short-term storage (up to 1 month): Store reconstituted protein at 4°C if it will be used within a short period. Adding a carrier protein such as BSA may extend short-term stability.
• Long-term storage: For extended storage, aliquot and freeze at -20°C or preferably -80°C. Lyophilized protein, if kept desiccated at -20°C, is generally stable for 12 months or longer from the date of receipt.
• Avoid: Repeated freeze-thaw cycles, exposure to direct light, and storage at temperatures above 4°C for extended periods.
• Desiccation: Keep lyophilized stocks in a desiccated environment to prevent moisture uptake, which can accelerate degradation.

Always record the date of receipt and first use. Discard any aliquots that show visible precipitation, cloudiness, or off-color appearance, as these may indicate degradation or contamination.

Research Applications

Recombinant Human CABCOCO1 Protein is suitable for a range of research applications. Researchers should validate the protein for each specific assay format in their own laboratory. Typical applications include:

• In Vitro Binding Assays: Used to study protein–protein interactions involving the CABCOCO1 coiled-coil domain or CoQ biosynthesis domain with known or putative binding partners.
• Antibody Validation: Serves as a positive control antigen for validating anti-CABCOCO1 antibodies by Western blot, ELISA, or dot blot.
• Structural and Biochemical Studies: Suitable for biophysical characterization, including circular dichroism (CD), size-exclusion chromatography (SEC), and thermal stability assays.
• Functional Assays: Applicable in enzymatic or co-factor binding assays investigating CABCOCO1's role in CoQ biosynthesis pathways.
• Cell-Free Systems: Can be used in cell-free transcription/translation validation or as a standard in quantitative assays.

Note: Optimal working concentrations and assay conditions should be determined empirically. Always include appropriate positive and negative controls in each experiment.

Quality, Safety, and Regulatory Notes

Research Use Only: Recombinant Human CABCOCO1 Protein (rp365597) is intended solely for in vitro research use. It is not approved for diagnostic, therapeutic, or veterinary use.

Quality Control: Each lot of Aladdin Scientific recombinant proteins undergoes quality control testing. Refer to the Certificate of Analysis (CoA) provided with each shipment for lot-specific purity, concentration, and activity data. The reference protocol number for this product is absea#PP-7948.

Safety: Handle all recombinant proteins in accordance with standard laboratory biosafety guidelines. While CABCOCO1 protein is not classified as a known biohazard, standard good laboratory practice (GLP) should be observed, including the use of appropriate personal protective equipment (PPE) such as gloves, lab coat, and eye protection.

Disposal: Dispose of protein solutions and contaminated materials in accordance with your institution's biosafety and chemical waste disposal policies.

Regulatory: This product is for research use only and has not been evaluated by any regulatory authority for clinical use. Users are responsible for compliance with all applicable local regulations.

Frequently asked questions

What is CABCOCO1 and why is it relevant to research?

CABCOCO1 (Coiled-coil and Coenzyme Q Biosynthesis Domain Containing Protein 1) is a human protein that features both coiled-coil structural motifs and a domain linked to coenzyme Q biosynthesis. It is of interest to researchers studying mitochondrial function, cellular energy metabolism, and related diseases. The recombinant form enables in vitro biochemical and structural investigations without reliance on endogenous expression systems.

How should I reconstitute Recombinant Human CABCOCO1 Protein?

Allow the lyophilized vial to reach room temperature before opening, then briefly centrifuge to collect all material. Reconstitute in the buffer and volume specified on the product datasheet (absea#PP-7948). Gently pipette to dissolve; do not vortex. Aliquot immediately into single-use portions and store at -80°C to avoid repeated freeze-thaw cycles.

What applications is Recombinant Human CABCOCO1 Protein validated for?

The protein is suitable for research applications including in vitro binding assays, antibody validation (Western blot, ELISA), structural and biophysical characterization, and functional studies related to CoQ biosynthesis. Researchers should empirically optimize concentrations and conditions for each specific assay, as performance may vary by experimental format.

What are the recommended storage conditions for this protein?

Store lyophilized protein at -20°C in a desiccated environment for long-term stability (up to 12 months or more from receipt). After reconstitution, aliquot and store at -80°C. For short-term use (within one month), reconstituted protein can be kept at 4°C. Avoid repeated freeze-thaw cycles, as they significantly diminish protein integrity and activity.

Is Recombinant Human CABCOCO1 Protein suitable for clinical or diagnostic use?

No. This product (rp365597) is strictly for in vitro research use only. It has not been evaluated or approved by any regulatory authority for diagnostic, therapeutic, or veterinary applications. Users are responsible for complying with all applicable institutional and governmental regulations.

References

1. Aladdin Scientific. Recombinant Human CABCOCO1 Protein, SKU rp365597. Product Datasheet absea#PP-7948.
2. Acosta Lopez MJ, et al. Coenzyme Q biosynthesis in health and disease. Biochim Biophys Acta Bioenerg. (General reference for CoQ biosynthesis pathway context — specific citation to be verified.)
3. UniProt Consortium. CABCOCO1 human protein entry. UniProtKB. (Accession number to be verified.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.