Recombinant Human CFAP298 Protein: Function & Research Use

Recombinant Human CFAP298 Protein is a cilia- and flagella-associated protein produced for use as a research reagent in studies of motile cilia assembly, axonemal ultrastructure, and ciliopathy-related disease mechanisms.

Protein Name

Recombinant Human CFAP298 (Cilia and Flagella Associated Protein 298)

SKU

rp373809

Species

Human (Homo sapiens)

Protein Family

Cilia and Flagella Associated Protein (CFAP) family

Primary Research Application

Motile cilia biology, ciliopathy mechanism studies, protein interaction assays

Expression System

Recombinant expression system (details subject to datasheet confirmation)

What Is CFAP298 and Its Biological Role?

CFAP298 (Cilia and Flagella Associated Protein 298) is a protein encoded by the CFAP298 gene in humans. It is specifically associated with motile cilia and flagella, organelles critical for fluid movement across epithelial surfaces and for sperm motility. CFAP298 localizes to the axoneme — the microtubule-based core structure of cilia — where it is thought to contribute to the proper assembly and maintenance of axonemal components including dynein arm complexes.

Motile cilia are essential in diverse physiological processes: mucociliary clearance in the respiratory tract, left–right organ patterning during embryogenesis, and cerebrospinal fluid circulation in brain ventricles. Disruption of genes encoding axonemal proteins, including members of the CFAP family, can lead to primary ciliary dyskinesia (PCD) and related ciliopathies, which are characterized by chronic respiratory infections, situs inversus, and infertility.

Research into CFAP298 expands our understanding of how individual axonemal proteins coordinate to generate and sustain ciliary beat patterns, making this protein a relevant subject for both basic and translational biomedical research.

Recombinant Protein Production and Key Biochemical Properties

Recombinant Human CFAP298 Protein (SKU: rp373809) is produced using recombinant expression technology, enabling researchers to obtain a well-defined, reproducible source of the protein outside its native cellular context. Recombinant production allows for controlled post-translational modification profiles and scalable quantities, which are important for biochemical assays, structural studies, and antibody validation.

Key considerations when working with recombinant CFAP298 include maintaining appropriate cold-chain storage conditions, avoiding repeated freeze–thaw cycles that can compromise protein integrity, and validating activity using suitable functional assays. Researchers are advised to consult the product-specific datasheet (referencing absea #PP-7655) for precise details on purity grade, formulation buffer, concentration, and recommended storage temperature.

As with other CFAP family recombinant proteins, CFAP298 is typically supplied in a formulation designed to preserve solubility and biological activity, and may include carrier proteins or stabilizing agents. Lot-specific certificates of analysis (CoA) provide quality control data including SDS-PAGE purity and endotoxin levels.

Research Applications of Recombinant Human CFAP298 Protein

Recombinant Human CFAP298 Protein is a versatile reagent applicable across several experimental formats in cilia and ciliopathy research:

• Protein–protein interaction studies: CFAP298 can be used in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance to identify and characterize binding partners within the axonemal proteome.
• Antibody development and validation: As a defined antigen, recombinant CFAP298 serves as an immunogen for generating specific antibodies and as a positive control for ELISA, Western blot, and immunofluorescence validation workflows.
• Structural and biophysical studies: Purified recombinant protein can be employed in techniques such as circular dichroism (CD) spectroscopy or analytical ultracentrifugation to characterize secondary structure and oligomeric state.
• Functional reconstitution assays: Researchers studying axonemal assembly in cell-free systems can incorporate recombinant CFAP298 to assess its contribution to dynein arm docking or outer doublet stability.
• Ciliopathy disease modeling: Recombinant CFAP298 supports mechanistic investigations into PCD pathogenesis, particularly in identifying how loss-of-function variants impair cilia structure or function.

Handling, Storage, and Quality Considerations

To ensure optimal performance of Recombinant Human CFAP298 Protein in downstream applications, researchers should adhere to the following best practices:

Storage: Upon receipt, the protein should be stored at the temperature specified in the product datasheet (typically −20 °C or −80 °C for long-term storage). Aliquoting into single-use volumes before the first freeze is strongly recommended to minimize the impact of freeze–thaw cycles on protein stability and activity.

Thawing: Thaw on ice or at 4 °C rather than at room temperature to reduce the risk of aggregation or denaturation.

Handling: Use low-binding microtubes and pipette tips to minimize protein loss due to adsorption. Avoid vigorous vortexing; mix gently by pipetting or brief low-speed centrifugation.

Quality verification: Confirm protein identity and purity using SDS-PAGE or Western blot with an appropriate anti-CFAP298 antibody prior to critical experiments. Refer to the lot-specific CoA for validated purity and concentration data.

Note: Always consult the product datasheet and CoA (absea #PP-7655) as the authoritative source for formulation, purity, and application-specific recommendations.

Frequently asked questions

What is CFAP298 and why is it important in cilia research?

CFAP298 is a cilia and flagella associated protein that localizes to the axoneme of motile cilia and flagella. It plays a role in axonemal assembly and is linked to ciliopathies such as primary ciliary dyskinesia (PCD) when mutated. Understanding CFAP298 function helps elucidate the molecular basis of motile cilia disorders.

What applications is Recombinant Human CFAP298 Protein (rp373809) suitable for?

Recombinant Human CFAP298 Protein is suitable for protein–protein interaction studies (e.g., pull-down, co-IP), antibody development and validation (ELISA, Western blot), structural and biophysical characterization, functional reconstitution assays, and mechanistic ciliopathy research. Always verify suitability for your specific application by consulting the product datasheet.

How should Recombinant Human CFAP298 Protein be stored to maintain activity?

The protein should be stored at the temperature recommended on the product datasheet (typically −20 °C or −80 °C for long-term storage). Aliquot into single-use volumes before freezing to avoid repeated freeze–thaw cycles, thaw on ice or at 4 °C, and use low-binding labware to minimize protein loss.

Is Recombinant Human CFAP298 Protein validated for Western blot or ELISA?

Validation data for specific applications are provided in the lot-specific certificate of analysis and product datasheet (absea #PP-7655). Researchers are advised to consult these documents for confirmed application data and to perform in-house validation using appropriate controls when establishing new assays.

What is the relationship between CFAP298 and primary ciliary dyskinesia (PCD)?

Mutations in cilia and flagella associated genes, including members of the CFAP family, have been linked to primary ciliary dyskinesia, a rare inherited disorder characterized by defective motile cilia function. Recombinant CFAP298 protein enables researchers to study the functional consequences of such mutations in biochemical and cell-free assay systems.

References

1. Wallmeier J, et al. Mutations in CCNO and MCIDAS underlie the mucociliary clearance disorder reduced generation of multiple motile cilia. Nature Genetics. 2014.
2. Reiter JF, Leroux MR. Genes and molecular pathways underpinning ciliopathies. Nature Reviews Molecular Cell Biology. 2017;18(9):533–547. doi:10.1038/nrm.2017.60
3. Mitchison HM, Valente EM. Motile and non-motile cilia in human pathology: from function to phenotypes. Journal of Pathology. 2017;241(2):294–309. doi:10.1002/path.4843
4. Aladdin Scientific. Recombinant Human CFAP298 Protein, SKU rp373809. Product Datasheet absea #PP-7655.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.