Recombinant Human GALP Protein: FAQs & Technical Guide

Recombinant Human GALP Protein is a bioactive galanin-like peptide produced recombinantly for use in neuroendocrine and metabolic research applications.

Product Name

Recombinant Human GALP Protein

SKU

rp367906

Protein Family

Galanin peptide family

Species Origin

Human (Homo sapiens)

Primary Research Area

Neuroendocrinology and metabolic regulation

Supplier

Aladdin Scientific

What Is Recombinant Human GALP Protein?

Galanin-like peptide (GALP) is a neuropeptide that shares structural homology with galanin and is encoded by the GALP gene in humans. It is predominantly expressed in the hypothalamus and pituitary gland, where it plays roles in regulating energy homeostasis, reproduction, and feeding behavior through interactions with galanin receptors (GALR1, GALR2, and GALR3).

Recombinant Human GALP Protein (SKU: rp367906) is a laboratory-grade, recombinantly expressed form of this peptide, manufactured to support in vitro and in vivo research applications. It enables scientists to study GALP signaling pathways, receptor binding, and downstream metabolic effects in controlled experimental systems.

Storage, Reconstitution, and Handling

Proper storage and handling are critical to maintaining the activity of Recombinant Human GALP Protein. As a general guideline for recombinant peptides of this class:

• Storage: Store lyophilized protein at −20°C or −80°C, protected from moisture and repeated freeze-thaw cycles.
• Reconstitution: Reconstitute in sterile, deionized water or a carrier protein-containing buffer (e.g., 0.1% BSA in PBS) to a working concentration appropriate for your assay. Allow the vial to equilibrate to room temperature before opening.
• Working aliquots: Prepare single-use aliquots after reconstitution and store at −80°C to minimize degradation.
• Avoid: Repeated freeze-thaw cycles, which can reduce bioactivity. Do not use if the solution appears turbid or has visible particulates.

Note: Always refer to the product-specific Certificate of Analysis (CoA) provided with your shipment for confirmed storage and reconstitution conditions for lot rp367906.

Research Applications of GALP Protein

Recombinant Human GALP Protein is used across a range of research disciplines. Key application areas include:

• Neuroendocrine research: Investigating GALP's role in hypothalamic signaling, luteinizing hormone (LH) release, and reproductive axis regulation.
• Metabolic studies: Examining GALP-mediated effects on food intake, body weight regulation, and energy expenditure in cell-based and animal models.
• Receptor binding assays: Characterizing binding affinity and selectivity for galanin receptor subtypes (GALR1, GALR2, GALR3) using competitive binding or surface plasmon resonance approaches.
• Cell signaling studies: Probing downstream intracellular signaling cascades activated by GALP, including cAMP modulation and MAP kinase pathways.
• Drug discovery: Serving as a reference ligand for screening compounds targeting galanin receptors in obesity, infertility, or neurological disease research programs.

Quality, Purity, and Regulatory Considerations

Aladdin Scientific's Recombinant Human GALP Protein (rp367906) is supplied as a research-grade reagent. Researchers should be aware of the following quality-related points:

• Purity: Recombinant proteins in this category are typically assessed by SDS-PAGE and/or HPLC. Refer to the lot-specific CoA for confirmed purity values for your shipment.
• Endotoxin: Endotoxin levels are typically evaluated by LAL assay. Low endotoxin preparations are recommended for cell-based functional assays. Consult the CoA for confirmed endotoxin specifications.
• Biological activity: Activity may be confirmed by functional assay (e.g., receptor binding or cell-based bioassay). Refer to the CoA or contact Aladdin Scientific technical support for activity data specific to your lot.
• Regulatory status: This product is intended for research use only (RUO). It is not approved for diagnostic, therapeutic, or veterinary use.

Ordering and Technical Support

Recombinant Human GALP Protein is available from Aladdin Scientific under SKU rp367906. To place an order or request a quote, visit the Aladdin Scientific website or contact your regional sales representative.

For technical questions regarding experimental design, reconstitution optimization, or compatibility with specific assay platforms, please contact the Aladdin Scientific technical support team. Our specialists can assist with:

• Selection of appropriate buffer systems and carrier proteins
• Recommended concentration ranges for common assay formats
• Lot-specific quality documentation requests
• Custom formulation inquiries

For returns, replacements, or quality complaints, please refer to Aladdin Scientific's standard returns and refunds policy and retain the original packaging and CoA documentation.

Frequently asked questions

What is GALP and why is it important in research?

GALP (Galanin-like peptide) is a neuropeptide structurally related to galanin that acts through galanin receptors GALR1, GALR2, and GALR3. It is primarily expressed in the hypothalamus and pituitary and is implicated in regulating energy balance, feeding behavior, body weight, and reproductive hormone secretion. Recombinant Human GALP Protein enables controlled in vitro and in vivo investigation of these physiological roles.

How should I reconstitute Recombinant Human GALP Protein for cell-based assays?

As a general recommendation, reconstitute lyophilized GALP protein in sterile deionized water or PBS containing a carrier protein such as 0.1% BSA to prevent non-specific adsorption and maintain stability. Allow the vial to equilibrate to room temperature before opening, and gently mix without vortexing. Always confirm the specific reconstitution conditions in the lot-specific Certificate of Analysis (CoA) supplied with SKU rp367906.

Is Recombinant Human GALP Protein suitable for in vivo studies?

Recombinant Human GALP Protein (rp367906) is a research-use-only (RUO) reagent and may be used in appropriately designed in vivo research models in compliance with institutional animal care guidelines. For in vivo applications, researchers should verify endotoxin levels from the lot-specific CoA and select preparations meeting their purity thresholds. This product is not approved for clinical, diagnostic, or therapeutic use in humans or animals.

What galanin receptor subtypes does GALP bind to?

GALP is known to bind to galanin receptor subtypes GALR1, GALR2, and GALR3, which are G protein-coupled receptors. The peptide shares the N-terminal sequence motif with galanin that is critical for receptor recognition. Recombinant Human GALP Protein can therefore be used in receptor binding studies to investigate subtype selectivity and pharmacological characterization.

How should I store Recombinant Human GALP Protein to maximize shelf life?

Store lyophilized Recombinant Human GALP Protein (rp367906) at −20°C or −80°C in a dry environment, away from light and moisture. After reconstitution, prepare single-use aliquots and store at −80°C. Avoid repeated freeze-thaw cycles, which can compromise bioactivity. Confirm exact storage conditions from the lot-specific CoA provided with your order.

References

1. Ohtaki T, et al. Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus. Journal of Biological Chemistry. 1999.
2. Krasnow SM, Hohmann JG, Clifton DK, Steiner RA. Molecular mechanisms regulating hypothalamic GALP expression. Peptides. 2003.
3. Cunningham MJ, et al. The role of galanin-like peptide in the neuroendocrine regulation of reproduction and body weight. Endocrinology. 2002.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.