Recombinant Human GBP3 Protein: FAQs & Key Facts

Recombinant Human GBP3 Protein is a recombinant form of the human guanylate-binding protein 3, a GTPase family member involved in innate immune defense and interferon-gamma-mediated signaling, offered by Aladdin Scientific to support immunology and cell biology research.

Product Name

Recombinant Human GBP3 Protein

SKU

rp374457

Protein Family

Guanylate-Binding Protein (GBP) family; large GTPase superfamily

Biological Function

Involved in innate immune response, interferon-gamma signaling, and intracellular pathogen defense

Supplier Reference

absea#PP-8261

Intended Use

Research use only (RUO); not for diagnostic or therapeutic use

What Is GBP3 and Why Is It Biologically Important?

Guanylate-Binding Protein 3 (GBP3) is a member of the large GTPase superfamily and belongs to the guanylate-binding protein family. GBPs are among the most abundantly induced proteins following interferon-gamma (IFN-γ) stimulation, and GBP3 plays a recognized role in innate immune defense against intracellular pathogens including bacteria, viruses, and parasites.

GBP3 hydrolyzes GTP to both GDP and GMP, a property that distinguishes it from many other GTPases. It participates in cell-autonomous immunity, contributing to the restriction of pathogen replication within host cells. Research into GBP3 function has implications for understanding inflammatory diseases, infectious disease biology, and interferon-mediated signaling pathways.

Product Specifications and Quality Attributes

Aladdin Scientific's Recombinant Human GBP3 Protein (SKU: rp374457) is produced as a recombinant protein intended for research applications. The product is referenced under supplier code absea#PP-8261. As with all recombinant proteins in the Aladdin catalog, researchers should consult the accompanying datasheet for confirmed specifications including molecular weight, host expression system, tag information, purity grade, and endotoxin levels.

Key quality parameters to verify on the datasheet include: expression host (commonly E. coli, insect, or mammalian cell systems), protein purity (typically assessed by SDS-PAGE and/or HPLC), endotoxin content (typically reported in EU/µg), and biological activity validation where applicable. These parameters directly influence downstream assay performance.

Storage, Handling, and Stability Recommendations

Recombinant proteins require careful handling to maintain activity and integrity. In the absence of product-specific data on the datasheet, the following general best practices apply to Recombinant Human GBP3 Protein:

• Short-term storage: Store at 4°C if the protein will be used within one to two weeks of receipt.
• Long-term storage: Aliquot the protein upon receipt to avoid repeated freeze-thaw cycles and store at −20°C or −80°C as indicated on the datasheet.
• Carrier protein: If storing at low concentrations, consider adding a carrier protein such as 0.1% BSA to prevent adsorption to tube surfaces.
• Avoid repeated freeze-thaw: Each freeze-thaw cycle can degrade protein activity; use single-use aliquots.

Always refer to the specific datasheet provided with your lot for confirmed storage buffer composition, recommended concentration range, and shelf life.

Research Applications of Recombinant Human GBP3 Protein

Recombinant Human GBP3 Protein is suitable for a range of in vitro research applications. Common uses include:

• Protein interaction studies: GBP3 can be used as bait or prey in pull-down assays and co-immunoprecipitation experiments to map protein-protein interactions within innate immune complexes.
• Antibody validation: Recombinant GBP3 serves as a positive control antigen for validating anti-GBP3 antibodies in Western blot, ELISA, and dot blot assays.
• Biochemical assays: GTPase activity assays can be performed using recombinant GBP3 to study its enzymatic properties and the effects of inhibitors or interacting partners.
• Structural studies: High-purity recombinant GBP3 may be used in structural biology workflows including crystallography or cryo-EM studies.
• Cell-based assays: Recombinant protein can be used to supplement cell-free systems or reconstitution experiments studying interferon-gamma pathway components.

Researchers should confirm suitability for each specific application by consulting the product datasheet and relevant literature.

Ordering, Documentation, and Regulatory Status

Recombinant Human GBP3 Protein is available from Aladdin Scientific under SKU rp374457. The product is intended for research use only (RUO) and is not approved or intended for use in diagnostic, therapeutic, or clinical procedures.

Upon ordering, customers typically receive a Certificate of Analysis (CoA) specific to the production lot, which documents purity, endotoxin levels, and activity data where applicable. A Safety Data Sheet (SDS) is available upon request. For bulk quantities, custom formulations, or specific quality documentation requirements, please contact Aladdin Scientific's technical support team.

Export of recombinant proteins may be subject to national and international regulatory requirements. Procurement teams should verify applicable regulations before placing international orders.

Frequently asked questions

What is the biological function of GBP3?

GBP3 (Guanylate-Binding Protein 3) is a large GTPase that is strongly induced by interferon-gamma. It plays a role in innate immune defense against intracellular pathogens, including bacteria, viruses, and parasites, by participating in cell-autonomous immunity mechanisms. It can hydrolyze GTP to both GDP and GMP.

What is the SKU for Recombinant Human GBP3 Protein from Aladdin Scientific?

The SKU for Recombinant Human GBP3 Protein from Aladdin Scientific is rp374457. The product is also referenced under supplier code absea#PP-8261. Please use this SKU when placing orders or making inquiries.

How should Recombinant Human GBP3 Protein be stored to maintain stability?

For short-term use within one to two weeks, store the protein at 4°C. For long-term storage, aliquot the protein to avoid repeated freeze-thaw cycles and store at −20°C or −80°C as specified on the product datasheet. Always consult the lot-specific Certificate of Analysis for confirmed storage conditions.

What applications is Recombinant Human GBP3 Protein validated for?

Recombinant Human GBP3 Protein is commonly used in antibody validation (Western blot, ELISA), protein-protein interaction studies (pull-down, co-IP), GTPase activity assays, and structural biology workflows. Specific validated applications are confirmed on the product datasheet; researchers should consult the datasheet and relevant literature for their intended use.

Is Recombinant Human GBP3 Protein suitable for clinical or diagnostic use?

No. Recombinant Human GBP3 Protein from Aladdin Scientific is designated for research use only (RUO) and is not approved, validated, or intended for diagnostic, therapeutic, or clinical applications.

Can I request bulk quantities or custom formulations of Recombinant Human GBP3 Protein?

Yes. For bulk quantities, custom buffer formulations, or specialized documentation requirements, please contact Aladdin Scientific's technical support or sales team directly. They can advise on feasibility, lead times, and pricing for non-standard orders.

References

1. Praefcke GJ, McMahon HT. The dynamin superfamily: universal membrane tubulation and fission molecules? Nature Reviews Molecular Cell Biology, 2004.
2. Vestal DJ, Jeyaratnam JA. The guanylate-binding proteins: emerging insights into the biochemical properties and functions of this family of large interferon-induced guanosine triphosphatase. Journal of Interferon & Cytokine Research, 2011.
3. Aladdin Scientific product datasheet for Recombinant Human GBP3 Protein, SKU rp374457 (absea#PP-8261).

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.