Recombinant Human GMPR2 Protein: Key Facts & FAQs

Recombinant Human GMPR2 Protein is a research-grade recombinant form of guanosine 5′-monophosphate reductase 2, an enzyme involved in purine nucleotide interconversion, offered by Aladdin Scientific (SKU: rp369687) to support biochemical, enzymological, and cell biology research applications.

Product Name

Recombinant Human GMPR2 Protein

SKU

rp369687

Protein Function

Catalyzes the NADPH-dependent reductive deamination of GMP to IMP, contributing to purine nucleotide interconversion

Source

Recombinant human protein expressed in a heterologous expression system

Intended Use

For research use only; not for therapeutic or diagnostic use

Supplier

Aladdin Scientific

What Is GMPR2 and Why Is It Important in Research?

GMPR2 (Guanosine 5′-Monophosphate Reductase 2) is an enzyme that catalyzes the irreversible, NADPH-dependent reductive deamination of guanosine 5′-monophosphate (GMP) to inosine 5′-monophosphate (IMP). This reaction sits at a critical branch point in purine nucleotide metabolism, governing the balance between adenine and guanine nucleotide pools within the cell.

GMPR2 is one of two human GMP reductase isoforms (GMPR and GMPR2) and has been studied in the context of cell proliferation, differentiation, and nucleotide homeostasis. Research into GMPR2 function is relevant to understanding cancers, immune cell biology, and the mechanisms of purine-targeting therapeutics.

Common Applications of Recombinant Human GMPR2 Protein

Recombinant Human GMPR2 Protein is suitable for a range of in vitro research applications, including:

• Enzyme activity assays: Measuring GMP reductase kinetics, substrate specificity, and inhibitor screening.
• Biochemical studies: Investigating protein–protein interactions, cofactor binding (NADPH, GMP), and allosteric regulation.
• Structural biology: Supporting crystallography or other structural studies when used as a reference or experimental protein.
• Antibody validation: Serving as a positive control antigen for validating GMPR2-specific antibodies in Western blot, ELISA, or immunoprecipitation.
• Drug discovery: Used as a target protein in high-throughput screening campaigns aimed at identifying GMPR2 inhibitors or modulators.

Researchers should confirm suitability for specific assays prior to use by consulting the product datasheet or contacting Aladdin Scientific technical support.

Storage, Handling, and Stability Guidelines

Proper storage and handling of Recombinant Human GMPR2 Protein are critical for maintaining protein integrity and biological activity. General best-practice recommendations for recombinant proteins of this class include:

• Storage temperature: Store at −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles, which can cause aggregation and loss of activity.
• Short-term storage: If the protein will be used within a few days, it may be stored at 4 °C. Consult the product datasheet for supplier-specified conditions.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimize freeze–thaw stress.
• Buffer compatibility: Check the formulation buffer provided with the product; adding a carrier protein (e.g., BSA) may improve stability during dilution.
• Handling: Work on ice whenever possible and avoid vortexing vigorously. Use low-binding tubes to minimize adsorption losses.

Always refer to the official product datasheet (SKU: rp369687) for Aladdin Scientific-specific storage instructions, as conditions may differ from general guidance.

Ordering and Technical Support Information

Recombinant Human GMPR2 Protein is available from Aladdin Scientific under SKU rp369687. To place an order or request a quote, researchers and procurement staff may:

• Visit the Aladdin Scientific website and search for SKU rp369687 directly.
• Contact the Aladdin Scientific sales team via the official website inquiry form or customer service email.
• For bulk or custom quantities, submit a custom request through the Aladdin Scientific procurement channel.

For technical questions regarding assay setup, protein activity validation, or compatibility with specific experimental workflows, contact Aladdin Scientific's technical support team. When reaching out, please have the SKU number (rp369687), your intended application, and your experimental conditions ready to facilitate an efficient response.

Frequently asked questions

What is the biological function of GMPR2?

GMPR2 (Guanosine 5′-Monophosphate Reductase 2) catalyzes the NADPH-dependent reductive deamination of GMP to IMP, playing a key role in regulating the balance of purine nucleotide pools. It is one of two human GMP reductase isoforms and is implicated in processes such as cell proliferation and nucleotide homeostasis.

Is Recombinant Human GMPR2 Protein suitable for use in enzyme inhibitor screening assays?

Yes, recombinant GMPR2 protein is commonly used as a target enzyme in biochemical inhibitor screening assays, including high-throughput formats. Researchers should optimize assay conditions such as protein concentration, cofactor (NADPH and GMP) concentrations, and buffer composition to achieve reliable results. Please consult the product datasheet or contact Aladdin Scientific technical support for specific recommendations.

How should I store Recombinant Human GMPR2 Protein to maintain its activity?

For long-term storage, keep Recombinant Human GMPR2 Protein at −80 °C and avoid repeated freeze–thaw cycles by preparing single-use aliquots upon first thaw. For short-term use within a few days, storage at 4 °C is generally acceptable. Always refer to the specific storage instructions on the Aladdin Scientific product datasheet for SKU rp369687.

Can Recombinant Human GMPR2 Protein be used as a positive control antigen for antibody validation?

Yes, recombinant proteins are widely used as positive control antigens in immunological assays such as Western blot, ELISA, and immunoprecipitation. Recombinant Human GMPR2 Protein can serve this purpose to validate the specificity and sensitivity of anti-GMPR2 antibodies. Verify that the protein tag or formulation does not interfere with the detection antibody before use.

How do I order Recombinant Human GMPR2 Protein from Aladdin Scientific?

Recombinant Human GMPR2 Protein can be ordered from Aladdin Scientific using SKU rp369687. Visit the Aladdin Scientific website to place an order directly, or contact the customer service team for assistance with quotes, bulk orders, or custom requests.

References

1. Dang L and Van Schaftingen E. (2002) Identification of two isoforms of guanosine monophosphate reductase in human cells. Biochemical Journal. This reference describes the characterization of GMPR2 as a distinct human isoform.
2. Hedstrom L. (2009) IMP Dehydrogenase: Structure, Mechanism, and Inhibition. Chemical Reviews. Provides background on purine nucleotide interconversion enzymes relevant to GMPR2 function.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.