Recombinant Human GMPS Protein: FAQs & Specifications

Recombinant Human GMPS Protein is a research-grade recombinant enzyme corresponding to human GMP synthetase, a key bifunctional enzyme catalyzing the final ATP-dependent amination step in de novo GMP biosynthesis from XMP.

Product Name

Recombinant Human GMPS Protein

SKU

rp364845

Gene Target

GMPS (GMP Synthetase)

Species Origin

Human (Homo sapiens)

Protein Function

Catalyzes conversion of XMP to GMP in de novo purine biosynthesis

Application

Biochemical assays, enzyme activity studies, protein interaction studies

What Is Recombinant Human GMPS Protein?

Recombinant Human GMPS Protein (SKU: rp364845) is a recombinant form of human GMP synthetase (GMPS), an enzyme encoded by the GMPS gene. GMPS catalyzes the conversion of xanthosine 5'-monophosphate (XMP) to guanosine 5'-monophosphate (GMP) in the de novo purine biosynthesis pathway. This reaction requires ATP and glutamine as substrates, making GMPS a bifunctional enzyme with both glutamine amidotransferase and ATP pyrophosphatase activities. The protein is essential for maintaining cellular GMP pools and, consequently, for DNA and RNA synthesis, cell proliferation, and signal transduction.

Aladdin Scientific supplies this protein as a research reagent suitable for in vitro biochemical and cell biology experiments.

Recommended Applications and Usage Guidelines

Recombinant Human GMPS Protein is recommended for the following research applications:

• Enzyme activity assays: Measuring GMP synthetase catalytic activity using XMP, glutamine, and ATP as substrates in standardized biochemical assays.
• Protein–protein interaction studies: Use as bait or prey in pull-down assays or co-immunoprecipitation experiments to identify GMPS binding partners.
• Structural and biophysical studies: Suitable as a reference standard or input material for techniques such as SPR, ITC, or X-ray crystallography.
• Drug discovery and inhibitor screening: Evaluating the potency and selectivity of candidate GMPS inhibitors relevant to oncology and infectious disease research.

Always reconstitute and handle the protein according to the product datasheet. Avoid repeated freeze-thaw cycles to maintain maximal activity.

Storage, Stability, and Handling

Proper storage is critical to preserving the biological activity of Recombinant Human GMPS Protein. The following general guidelines apply to recombinant proteins of this class:

• Short-term storage (up to 1 month): Store at 4 °C if the protein will be used within a short period after reconstitution.
• Long-term storage: Aliquot and store at −20 °C or −80 °C to prevent activity loss from repeated freeze-thaw cycles.
• Carrier protein: Addition of a carrier protein such as BSA (0.1%) is recommended for dilute preparations to minimize adsorption to tube surfaces.
• Avoid: Repeated freeze-thaw cycles, exposure to detergents, and prolonged incubation at room temperature.

Consult the product-specific datasheet for exact buffer composition, recommended storage conditions, and shelf-life information, as these may vary by lot.

Quality, Purity, and Regulatory Information

Aladdin Scientific's Recombinant Human GMPS Protein is produced and quality-controlled to meet the demands of research-grade applications. Key quality attributes typically assessed for recombinant proteins in this category include:

• Purity: Assessed by SDS-PAGE and/or HPLC. Research-grade recombinant proteins are generally supplied at ≥85–95% purity, though exact specifications should be confirmed on the product datasheet or certificate of analysis (CoA).
• Identity: Confirmed by western blot or mass spectrometry against the target sequence.
• Biological activity: Verified by functional enzyme assay where applicable.
• Endotoxin level: Typically tested by LAL assay; consult CoA for lot-specific values.

This product is intended exclusively for research use only (RUO) and is not suitable for diagnostic, therapeutic, or clinical use in humans or animals.

Ordering, Support, and Technical Assistance

Recombinant Human GMPS Protein (SKU: rp364845) can be ordered directly through the Aladdin Scientific website or by contacting our sales team. For pricing, availability, and bulk or custom quantity inquiries, please reach out to your regional Aladdin Scientific representative.

Technical support is available for questions regarding reconstitution protocols, application optimization, and compatibility with specific assay formats. Our scientific support team can assist with:

• Recommended buffer and assay conditions for GMPS enzyme activity studies
• Guidance on appropriate positive and negative controls
• Troubleshooting low activity or unexpected results
• Documentation requests including Safety Data Sheets (SDS) and Certificates of Analysis (CoA)

Contact us via the Aladdin Scientific support portal or email for prompt assistance.

Frequently asked questions

What is the biological function of GMPS?

GMPS (GMP synthetase) catalyzes the ATP-dependent conversion of xanthosine 5'-monophosphate (XMP) to guanosine 5'-monophosphate (GMP) using glutamine as the nitrogen donor. This is the final committed step in the de novo biosynthesis of GMP, an essential purine nucleotide required for DNA and RNA synthesis, energy metabolism, and cell signaling.

What expression system is used to produce Recombinant Human GMPS Protein?

The specific expression system for this product (rp364845) should be confirmed by consulting the product datasheet or contacting Aladdin Scientific technical support. Common expression systems for human recombinant proteins include E. coli, baculovirus/insect cells, and HEK293 mammalian cells, each offering different profiles of post-translational modifications and activity.

How should I reconstitute and store Recombinant Human GMPS Protein?

Reconstitute the lyophilized protein by adding sterile deionized water or the buffer specified in the product datasheet to the stated concentration, then gently mix without vortexing. Aliquot into single-use volumes to avoid repeated freeze-thaw cycles. Store aliquots at −20 °C or −80 °C for long-term stability, and at 4 °C for short-term use within one month. Refer to the lot-specific CoA for exact storage conditions.

Is this protein suitable for use in drug discovery or inhibitor screening?

Yes, Recombinant Human GMPS Protein is suitable for in vitro inhibitor screening assays. GMPS is considered a potential drug target in oncology, as rapidly proliferating cancer cells have elevated requirements for GMP biosynthesis. Biochemical assays measuring XMP-to-GMP conversion can be used to evaluate inhibitor potency and selectivity against the human enzyme.

What quality controls are performed on this product?

Research-grade recombinant proteins from Aladdin Scientific are typically assessed for purity by SDS-PAGE, protein identity by western blot or mass spectrometry, biological activity by functional assay, and endotoxin levels by LAL assay. Please refer to the Certificate of Analysis (CoA) for lot-specific quality control data.

References

1. Tesmer JJ, Klem TJ, Deras ML, Davisson VJ, Smith JL. The crystal structure of GMP synthetase reveals a novel catalytic triad and is a structural paradigm for three enzymes of the triad synthetase family. Nature Structural Biology. 1996;3(1):74-86.
2. Nakamura J, Lou L. Biochemical characterization of human GMP synthetase. Journal of Biological Chemistry. 1995;270(13):7347-7353.
3. Aladdin Scientific. Recombinant Human GMPS Protein, SKU rp364845. Product Datasheet. Aladdin Scientific; [date accessed: 2024].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.