Recombinant Human GOLGA8IP Protein: Overview & Research Use

Recombinant Human GOLGA8IP Protein is a research-grade recombinant protein derived from the human GOLGA8IP gene, designed to support investigators studying Golgi apparatus organization, golgin family interactions, and related intracellular trafficking mechanisms.

Product Name

Recombinant Human GOLGA8IP Protein

SKU

rp376091

Gene Name

GOLGA8IP (Golgin Subfamily A Member 8 Family-Interacting Protein)

Protein Origin

Human (Homo sapiens)

Product Format

Recombinant protein; specific formulation details should be confirmed with product datasheet

Intended Use

Research use only (RUO); not for diagnostic or therapeutic applications

Biological Background of GOLGA8IP

GOLGA8IP, also referred to as Golgin Subfamily A Member 8 Family-Interacting Protein, belongs to the broader golgin protein superfamily. Golgins are a class of coiled-coil domain-containing proteins predominantly associated with the cytoplasmic face of the Golgi apparatus. They play essential roles in maintaining Golgi stack integrity, mediating vesicle tethering, and facilitating the directional trafficking of cargo between Golgi cisternae and other cellular compartments.

The GOLGA8 subfamily, to which GOLGA8IP is functionally related, is notable for its association with Golgi structure and membrane dynamics. Although GOLGA8IP is less extensively characterized than classical golgins such as GM130 or giantin, it is believed to participate in protein-protein interactions within the Golgi network, potentially contributing to the spatial organization of this organelle. Research into GOLGA8IP and its interaction partners may yield important insights into Golgi biogenesis, inheritance during cell division, and the pathological consequences of Golgi fragmentation observed in neurodegenerative diseases and cancer.

Note: Detailed functional characterization of GOLGA8IP remains an active area of investigation. Researchers are encouraged to consult primary literature for the most current findings.

Protein Characteristics & Specifications

Recombinant Human GOLGA8IP Protein (SKU: rp376091) is produced using recombinant expression technology to yield a highly pure protein suitable for a wide range of in vitro research applications. Key specifications to review on the official product datasheet include:

• Expression System: The specific host expression system (e.g., E. coli, mammalian, baculovirus) should be verified on the current product datasheet, as this affects post-translational modification status and protein folding.
• Purity: Research-grade recombinant proteins from Aladdin Scientific are typically supplied at high purity, generally assessed by SDS-PAGE and/or HPLC. Exact purity figures should be confirmed via the product datasheet or Certificate of Analysis (CoA).
• Formulation: The protein is supplied in a buffered solution optimized for stability. Storage conditions and recommended handling procedures are detailed on the product-specific datasheet.
• Molecular Weight: The apparent and theoretical molecular weights should be confirmed on the product datasheet, as recombinant constructs may include affinity tags that alter the observed molecular weight.

All lot-specific quality control data, including purity assessment results and endotoxin levels where applicable, are available upon request or included in the CoA shipped with the product.

Research Applications

Recombinant Human GOLGA8IP Protein is intended for research use only and may be suitable for the following in vitro laboratory applications, subject to individual laboratory validation:

• Protein-Protein Interaction Studies: Can be used in pull-down assays, co-immunoprecipitation experiments, or surface plasmon resonance (SPR) studies to identify and characterize binding partners of GOLGA8IP within the Golgi interaction network.
• Antibody Development & Validation: Suitable as an antigen for the generation of polyclonal or monoclonal antibodies targeting GOLGA8IP, or as a positive control in Western blot and ELISA validation workflows.
• Structural Biology: High-purity recombinant protein may be applicable to biophysical characterization techniques such as circular dichroism (CD) spectroscopy to assess secondary structure, or dynamic light scattering (DLS) to evaluate solution behavior.
• Functional Assays: Depending on experimental design, the protein may be used to study its role in Golgi-associated membrane tethering or vesicle trafficking reconstitution assays in cell-free systems.

Researchers should perform application-specific optimization and include appropriate controls. For technical support and application recommendations, contact Aladdin Scientific technical services.

Storage, Handling & Quality Assurance

Proper storage and handling of recombinant proteins are critical to maintaining biological activity and ensuring reproducible experimental results. General best-practice guidelines for Recombinant Human GOLGA8IP Protein include:

• Storage Temperature: Recombinant proteins are typically stored at -20°C or -80°C upon receipt. Refer to the product-specific datasheet for the recommended storage temperature for this product.
• Avoid Repeated Freeze-Thaw Cycles: Aliquot the protein into single-use volumes upon first thaw to minimize activity loss from repeated freeze-thaw cycles.
• Carrier Protein: If long-term stability is a concern, addition of a carrier protein such as BSA (bovine serum albumin) may be recommended — consult the datasheet for guidance.
• Quality Control: Each lot undergoes quality control testing. Typical QC assays may include SDS-PAGE for purity confirmation and identity verification. Lot-specific CoA documentation is available.

For the most accurate and up-to-date storage and handling information, always refer to the product datasheet and CoA supplied with each specific lot of rp376091.

Frequently asked questions

What is GOLGA8IP and why is it relevant to Golgi research?

GOLGA8IP is a member of the golgin protein superfamily that associates with the Golgi apparatus. Golgins, including GOLGA8IP, are involved in maintaining Golgi structural integrity and facilitating intracellular vesicle trafficking. Understanding GOLGA8IP interactions may provide insights into Golgi biogenesis, Golgi inheritance during cell division, and disease states linked to Golgi dysfunction.

What applications is Recombinant Human GOLGA8IP Protein (rp376091) suitable for?

Recombinant Human GOLGA8IP Protein is suitable for research applications including protein-protein interaction studies (pull-down, co-IP, SPR), antibody generation and validation (Western blot, ELISA positive controls), structural biology techniques (CD spectroscopy, DLS), and in vitro functional assays. All applications should be validated by the researcher in their specific experimental system.

How should Recombinant Human GOLGA8IP Protein be stored to maintain stability?

Recombinant proteins should generally be stored at -20°C or -80°C and repeated freeze-thaw cycles should be avoided by aliquoting into single-use volumes upon first thaw. For product rp376091, always refer to the specific product datasheet and Certificate of Analysis for the manufacturer-recommended storage conditions, as formulation details may vary by lot.

Is Recombinant Human GOLGA8IP Protein (rp376091) suitable for in vivo or diagnostic use?

No. Recombinant Human GOLGA8IP Protein (rp376091) is supplied for research use only (RUO) and is not intended for diagnostic, therapeutic, or in vivo applications in humans or animals. Researchers must comply with all applicable institutional and regulatory guidelines when handling this product.

References

1. Munro S. The golgin coiled-coil proteins of the Golgi apparatus. Cold Spring Harb Perspect Biol. 2011;3(6):a005256. doi:10.1101/cshperspect.a005256
2. Barr FA, Short B. Golgins in the structure and dynamics of the Golgi apparatus. Curr Opin Cell Biol. 2003;15(4):405-413. doi:10.1016/S0955-0674(03)00054-1
3. Aladdin Scientific. Recombinant Human GOLGA8IP Protein (rp376091) Product Datasheet. Aladdin Scientific; [current version].

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.