Recombinant Human GPBP1 Protein: Properties & Research Use

Recombinant Human GPBP1 Protein is a research-grade recombinant protein designed to support investigations into GPBP1-mediated biological processes, including its roles in cellular signaling and tissue-specific functions.

Product Name

Recombinant Human GPBP1 Protein

SKU

rp374093

Species Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Reference Catalog

absea#PP-7928

Intended Use

Research use only; not for diagnostic or therapeutic purposes

What Is GPBP1 and Why Does It Matter in Research?

GPBP1, also known as Goodpasture Antigen-Binding Protein 1, is a serine/threonine kinase family member that has attracted significant interest in biomedical research due to its involvement in extracellular matrix organization, cellular signaling cascades, and tissue homeostasis. Originally identified through its interaction with the Goodpasture antigen (the alpha-3 chain of type IV collagen), GPBP1 has since been implicated in cardiac muscle function, inflammation regulation, and nuclear transport mechanisms.

Research on GPBP1 spans multiple disease contexts, including autoimmune conditions such as Goodpasture syndrome, as well as cardiomyopathies and inflammatory disorders. Understanding the molecular behavior of GPBP1 requires access to high-quality, well-characterized recombinant protein, making Recombinant Human GPBP1 Protein (rp374093) a valuable reagent for in vitro biochemical and cell-based assays.

Biochemical Characteristics of Recombinant Human GPBP1 Protein

Recombinant Human GPBP1 Protein is produced using recombinant expression systems optimized to deliver a folded, functional protein suitable for downstream research applications. As a recombinant reagent, it enables reproducible experimental results compared to protein extracted directly from human tissues.

Key biochemical considerations for working with this protein include its solubility profile, storage buffer composition, and recommended handling temperatures. Researchers should consult the product datasheet (referencing absea catalog number PP-7928) for lot-specific purity data, molecular weight confirmation by SDS-PAGE, and endotoxin levels. Proper storage at recommended temperatures—typically -80°C for long-term storage with avoidance of repeated freeze-thaw cycles—is critical to maintaining protein activity and integrity.

As with all recombinant proteins, users should validate activity in their specific assay system before large-scale experimental use, as matrix effects and assay conditions can influence observed performance.

Research Applications of GPBP1 Recombinant Protein

Recombinant Human GPBP1 Protein supports a variety of research applications where a defined, pure protein source is required. Common uses include:

• Binding and interaction studies: Investigating protein-protein interactions between GPBP1 and its known binding partners, including type IV collagen and other extracellular matrix components.
• Enzyme activity assays: Characterizing the kinase activity of GPBP1 and identifying potential substrate proteins or small-molecule modulators.
• Antibody development and validation: Serving as an antigen for raising or validating anti-GPBP1 antibodies used in Western blot, ELISA, or immunohistochemistry.
• Structural biology: Supporting crystallography or cryo-EM studies to elucidate the three-dimensional structure of GPBP1 and its complexes.
• Disease model studies: Facilitating mechanistic research into GPBP1's role in autoimmune kidney disease, cardiomyopathy, or inflammatory pathways.

This protein is intended strictly for research use only and is not approved for clinical, diagnostic, or therapeutic applications.

Handling, Storage, and Quality Considerations

To maximize the utility and longevity of Recombinant Human GPBP1 Protein, researchers should adhere to the following best practices:

• Storage: Store at -80°C upon receipt for long-term preservation. Aliquot the protein into single-use volumes to minimize freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Reconstitution: If supplied lyophilized, reconstitute in the buffer specified on the product datasheet. Allow the vial to equilibrate at room temperature briefly before opening to prevent condensation-related contamination.
• Working concentrations: Determine optimal working concentrations empirically for each assay format, as requirements vary significantly between ELISA, kinase assay, or binding studies.
• Quality control: Each lot of rp374093 should be accompanied by a certificate of analysis (CoA) confirming purity (typically assessed by SDS-PAGE and/or HPLC) and biological activity data where applicable. Contact Aladdin Scientific for lot-specific CoA documentation.

Researchers are encouraged to cite the catalog number rp374093 in publications to ensure reproducibility and traceability of their experimental materials.

Frequently asked questions

What is the recommended storage condition for Recombinant Human GPBP1 Protein (rp374093)?

For long-term storage, Recombinant Human GPBP1 Protein should be stored at -80°C. To preserve activity, aliquot the protein into small, single-use volumes upon receipt and avoid repeated freeze-thaw cycles. Always refer to the specific product datasheet or certificate of analysis for lot-specific storage recommendations.

What research applications is Recombinant Human GPBP1 Protein suitable for?

Recombinant Human GPBP1 Protein (rp374093) is suitable for a range of in vitro research applications, including protein-protein interaction studies, kinase activity assays, antibody development and validation, and structural biology investigations. It is for research use only and is not intended for diagnostic or therapeutic use.

How should I determine the optimal working concentration of GPBP1 recombinant protein for my assay?

Optimal working concentrations for Recombinant Human GPBP1 Protein vary depending on the assay format and experimental system used. Researchers should perform a titration experiment using a range of protein concentrations to identify the optimal dose-response in their specific assay. Consulting the product datasheet for any suggested starting concentrations is also recommended.

Can I use Recombinant Human GPBP1 Protein for in vivo studies or clinical applications?

No. Recombinant Human GPBP1 Protein (rp374093) is strictly for in vitro research use only. It has not been evaluated or approved for use in clinical, diagnostic, or therapeutic settings, and must not be administered to humans or animals in any clinical context.

How can I obtain a Certificate of Analysis (CoA) for my lot of rp374093?

Lot-specific Certificates of Analysis for Recombinant Human GPBP1 Protein (rp374093) can be obtained by contacting Aladdin Scientific's customer service team with your lot number. The CoA will include purity assessment data and any available activity information for that specific production lot.

References

1. Product reference: absea catalog number PP-7928, Recombinant Human GPBP1 Protein. Manufacturer's product documentation.
2. Raya A, et al. Identification of a novel serine/threonine kinase as a putative intermediate in signaling by members of the nuclear receptor family. Molecular and Cellular Biology. (General reference for GPBP kinase family context — specific citation requires verification.)
3. Hudson BG, et al. Goodpasture's syndrome: molecular architecture and function of basement membrane antigen. Laboratory Investigation. (General reference for Goodpasture antigen context — specific citation requires verification.)

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.