Recombinant Human GRAMD4 Protein: FAQs & Key Facts

Recombinant Human GRAMD4 Protein is a research-grade recombinant protein derived from the human GRAMD4 gene, intended for use in studies of apoptosis regulation, lipid sensing, and related cellular signaling pathways.

Product Name

Recombinant Human GRAMD4 Protein

SKU

rp366743

Gene Name

GRAMD4 (GRAM Domain Containing 4)

Species

Human (Homo sapiens)

Protein Class

GRAM domain-containing protein involved in apoptosis signaling

Supplier

Aladdin Scientific

What Is GRAMD4 and Why Is It Studied?

GRAMD4 (GRAM Domain Containing Protein 4) is a human protein characterized by the presence of a GRAM (Glucosyltransferase, Rab-like GTPase Activators, and Myotubularins) domain. This domain is structurally related to the pleckstrin homology (PH) domain and is implicated in lipid binding and membrane association. GRAMD4 has been reported to function as a p53-inducible gene product involved in promoting apoptosis, making it relevant to cancer biology, cell death signaling, and stress-response research. Researchers studying tumor suppressor pathways, mitochondrial apoptosis, or lipid-mediated signaling commonly work with GRAMD4.

Product Specifications and Quality

Recombinant Human GRAMD4 Protein (SKU: rp366743) is produced for research use. The product is manufactured using recombinant expression technology to ensure batch-to-batch consistency. Purity is assessed by SDS-PAGE and other standard quality control methods. Researchers should consult the product datasheet or contact Aladdin Scientific technical support for the most current specifications, including exact purity percentage, molecular weight of the expressed construct, endotoxin levels, and formulation buffer composition, as these details are subject to lot-specific variation and are confirmed at the time of manufacture.

Recommended Storage and Handling

Proper storage is critical to maintaining the activity and integrity of recombinant proteins. As a general best practice for recombinant proteins, Recombinant Human GRAMD4 Protein should be stored according to the conditions specified on the product datasheet provided with each lot. Typically, recombinant proteins are stored at -20°C or -80°C in aliquots to avoid repeated freeze-thaw cycles, which can compromise protein integrity and activity. Addition of a carrier protein such as BSA may be recommended for long-term storage of low-concentration preparations. Always centrifuge vials briefly upon receipt before opening, and handle on ice during experimental use. Refer to the specific lot datasheet for confirmed storage instructions.

Common Research Applications

Recombinant Human GRAMD4 Protein is intended for in vitro research applications. Potential uses include: (1) Functional assays — studying GRAMD4's role in apoptosis induction and p53-dependent signaling; (2) Biochemical assays — protein-protein interaction studies, lipid-binding assays, and ELISA development; (3) Antibody validation — use as a positive control antigen for GRAMD4-specific antibodies in Western blot or ELISA; (4) Structural studies — investigation of GRAM domain architecture and lipid recognition. This product is not intended for diagnostic or therapeutic use in humans or animals. Researchers should validate the protein in their specific assay system prior to use in critical experiments.

Ordering and Technical Support

Recombinant Human GRAMD4 Protein is available from Aladdin Scientific under SKU rp366743. To place an order, visit the Aladdin Scientific website or contact your regional sales representative. For technical questions regarding protein activity, assay compatibility, or custom formulation requests, please reach out to the Aladdin Scientific technical support team. Volume pricing and institutional purchasing arrangements may be available — contact the sales team for details. All products are intended for research use only (RUO) and are not for resale without authorization.

Frequently asked questions

What is the biological function of GRAMD4?

GRAMD4 is a GRAM domain-containing protein reported to act as a p53-inducible pro-apoptotic factor. It is implicated in promoting programmed cell death and has been associated with lipid-binding activity via its GRAM domain, which is structurally related to pleckstrin homology (PH) domains. Its precise mechanistic role in apoptosis signaling remains an active area of investigation.

What expression system is used to produce Recombinant Human GRAMD4 Protein?

The specific expression system (e.g., E. coli, baculovirus/insect cell, mammalian cell, or yeast) used for Recombinant Human GRAMD4 Protein (rp366743) is detailed in the product datasheet accompanying each lot. Please consult the datasheet or contact Aladdin Scientific technical support for confirmed expression system information for your specific lot.

How should I store Recombinant Human GRAMD4 Protein to maintain its activity?

Recombinant proteins should generally be stored in aliquots at -20°C or -80°C to minimize freeze-thaw cycles and preserve activity. Always refer to the specific lot datasheet for confirmed storage temperature, buffer composition, and recommended working concentration. Avoid repeated freeze-thaw cycles and keep protein on ice during experimental use.

Is Recombinant Human GRAMD4 Protein suitable for in vivo or clinical use?

No. Recombinant Human GRAMD4 Protein (rp366743) is labeled for research use only (RUO). It is not approved, validated, or intended for use in diagnostic procedures, therapeutic applications, or any clinical setting involving humans or animals.

Can I use this protein as a positive control in Western blot or ELISA?

Yes, recombinant proteins are commonly used as positive control antigens for antibody validation in Western blot and ELISA assays. Recombinant Human GRAMD4 Protein can serve this purpose for GRAMD4-specific antibody validation. However, researchers should verify the molecular weight of the recombinant construct versus endogenous GRAMD4, as tag sequences or truncations may affect apparent molecular weight on SDS-PAGE.

How do I order Recombinant Human GRAMD4 Protein from Aladdin Scientific?

Recombinant Human GRAMD4 Protein can be ordered directly from the Aladdin Scientific website using SKU rp366743, or by contacting your regional Aladdin Scientific sales representative. For bulk orders or institutional purchasing, please contact the sales team to discuss pricing and availability.

References

1. Uhlen M, et al. A pathology atlas of the human cancer transcriptome. Science. 2017. doi:10.1126/science.aan2507
2. Katoh M, Katoh M. Identification and characterization of human GRAMD4 gene in silico. Int J Oncol. 2003.
3. Product datasheet: Recombinant Human GRAMD4 Protein, SKU rp366743. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.