Recombinant Human GRHL3 Protein: FAQs & Key Facts

Recombinant Human GRHL3 Protein is a recombinant form of the grainyhead-like transcription factor 3, an essential regulator of epithelial barrier integrity and epidermal differentiation, supplied by Aladdin Scientific to support a wide range of in vitro biochemical and cell-biology research applications.

Product Name

Recombinant Human GRHL3 Protein

SKU

rp367844

Protein Family

Grainyhead-like (GRHL) transcription factor family

Biological Function

Regulates epithelial barrier formation, epidermal differentiation, and wound healing

Expression Host

Recombinant expression system (verify specific host with product datasheet)

Recommended Storage

Store at -80 °C upon receipt; avoid repeated freeze-thaw cycles (verify with product datasheet)

Purity

Refer to product datasheet for lot-specific purity data

What Is GRHL3 and Why Is It Important in Research?

GRHL3 (Grainyhead-like protein 3) is a member of the evolutionarily conserved grainyhead-like family of transcription factors, which play critical roles in epithelial development and homeostasis. In humans, GRHL3 is highly expressed in stratified epithelia, including the epidermis, where it drives terminal differentiation and the formation of a competent skin barrier. Loss-of-function mutations in GRHL3 have been associated with spina bifida, palmoplantar keratoderma, and impaired wound healing, highlighting its clinical relevance.

From a research perspective, GRHL3 is studied in the contexts of epidermal barrier biology, neural tube closure, cancer (particularly squamous cell carcinoma), and transcriptional regulatory networks. Recombinant Human GRHL3 Protein enables direct in vitro investigation of its DNA-binding properties, protein–protein interactions, and downstream signaling without the complexity of cell-based overexpression systems.

Product Specifications and Quality Attributes

Recombinant Human GRHL3 Protein (SKU: rp367844) is produced using a recombinant expression system optimized for yield and functional integrity. Key quality attributes to review on the accompanying Certificate of Analysis (CoA) and product datasheet include:

• Purity: Assessed by SDS-PAGE and/or HPLC; lot-specific purity values are provided on the CoA.
• Molecular weight: Verify the apparent molecular weight on the datasheet, as recombinant constructs may include affinity tags that shift the observed band.
• Endotoxin level: Critical for cell-based assays; confirm endotoxin specification on the CoA before use in sensitive biological systems.
• Formulation: Buffer composition, carrier protein content (if any), and protein concentration are listed on the datasheet; review prior to experimental design.
• Activity: Functional activity data (e.g., DNA-binding ELISA or reporter assay) may be provided; consult the datasheet or contact Aladdin Scientific technical support for lot-specific data.

Always request the most current CoA for the specific lot received to ensure data accuracy.

Recommended Applications and Experimental Use

Recombinant Human GRHL3 Protein is suitable for multiple research applications, subject to validation in the user's specific system. Common applications include:

• Biochemical assays: ELISA-based interaction studies, electrophoretic mobility shift assays (EMSA) to characterize DNA-binding specificity, and pull-down assays to map protein interaction partners.
• Structural studies: As a purified recombinant protein, GRHL3 can serve as antigen for antibody generation or as a reference standard in Western blot calibration.
• Drug discovery: Used as a target protein in inhibitor or activator screening assays relevant to epithelial cancers or barrier disorders.
• Cell-free transcription/translation systems: May be incorporated into reconstituted transcriptional assays to dissect cofactor requirements.

Researchers are advised to perform application-specific optimization and include appropriate positive and negative controls. Contact Aladdin Scientific technical support if guidance on assay design is needed.

Storage, Handling, and Stability Guidelines

Correct handling of Recombinant Human GRHL3 Protein is essential to preserve its activity and ensure reproducible results. Follow these general guidelines (always defer to the product datasheet for lot-specific instructions):

• Upon receipt: Inspect the package immediately. If the protein was shipped on dry ice, allow it to equilibrate briefly before opening to avoid condensation-related contamination.
• Long-term storage: Aliquot into single-use volumes and store at −80 °C to minimize activity loss from freeze-thaw cycling.
• Short-term storage: If working stock is needed, store at 4 °C for up to the period specified in the datasheet; do not exceed this duration.
• Freeze-thaw cycles: Repeated freeze-thaw cycles can cause protein aggregation and loss of activity. Prepare single-use aliquots before freezing.
• Carrier protein: If the formulation lacks a stabilizing carrier such as BSA, consider adding one when preparing dilute working stocks, following datasheet guidance.
• Sterile technique: Use sterile, low-binding tubes and pipette tips to prevent adsorption and microbial contamination, particularly for cell-based assays.

Ordering, Regulatory Status, and Technical Support

How to Order: Recombinant Human GRHL3 Protein is available from Aladdin Scientific under SKU rp367844. Orders can be placed through the Aladdin Scientific website, by contacting your regional sales representative, or via your institution's approved procurement portal. For bulk or custom quantity inquiries, contact the Aladdin Scientific sales team directly.

Regulatory Status: This product is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or clinical use in humans or animals. Users are responsible for ensuring compliance with all applicable institutional and governmental regulations when using this reagent.

Technical Support: Aladdin Scientific's technical support team is available to assist with experimental design, troubleshooting, and application-specific questions. When contacting support, please have your lot number and CoA available to expedite assistance.

Returns and Replacements: For damaged, defective, or incorrectly shipped items, contact Aladdin Scientific customer service promptly. Refer to the Aladdin Scientific returns and refund policy for eligibility criteria and procedures.

Frequently asked questions

What is the biological function of human GRHL3 protein?

GRHL3 is a transcription factor belonging to the grainyhead-like family that regulates epithelial barrier formation, epidermal terminal differentiation, neural tube closure, and wound healing. It binds specific DNA sequences to activate or repress target genes involved in keratinocyte maturation and barrier competence. Dysregulation of GRHL3 has been linked to spina bifida, skin barrier disorders, and squamous cell carcinoma.

What expression system is used to produce Recombinant Human GRHL3 Protein (rp367844)?

The specific expression host for rp367844 is detailed on the product datasheet and Certificate of Analysis available from Aladdin Scientific. Common recombinant expression systems for transcription factors include E. coli, baculovirus/insect cell, and mammalian cell platforms, each offering different post-translational modification profiles. Please consult the datasheet or contact Aladdin Scientific technical support to confirm the expression system for your specific lot.

What applications is Recombinant Human GRHL3 Protein validated for?

Recombinant Human GRHL3 Protein is suitable for biochemical applications such as EMSA, protein interaction studies, ELISA-based binding assays, and use as an antigen for antibody production or a reference standard in Western blot. Suitability for specific applications should be confirmed against the product datasheet, and end-user validation in the target assay system is always recommended.

How should Recombinant Human GRHL3 Protein be stored to maintain activity?

Upon receipt, the protein should be aliquoted into single-use volumes and stored at −80 °C for long-term preservation. Repeated freeze-thaw cycles should be strictly avoided as they promote protein aggregation and activity loss. Working aliquots may be kept at 4 °C for short periods as specified in the product datasheet.

Is Recombinant Human GRHL3 Protein (rp367844) suitable for clinical or diagnostic use?

No. Recombinant Human GRHL3 Protein (rp367844) is supplied for research use only (RUO) and is not intended or approved for diagnostic, therapeutic, or clinical applications in humans or animals. Researchers must comply with all relevant institutional biosafety and regulatory requirements when handling this material.

How can I obtain a Certificate of Analysis (CoA) for my lot of rp367844?

A Certificate of Analysis specific to your lot number can be requested directly from Aladdin Scientific through the product page on the website or by contacting customer service with your lot number. The CoA contains lot-specific data including purity, concentration, endotoxin level, and quality control test results.

References

1. Ting SB, Caddy J, Hislop N, et al. A homolog of Drosophila grainy head is essential for epidermal integrity in mice. Science. 2005;308(5720):411-413. doi:10.1126/science.1107511
2. Boglev Y, Wilanowski T, Caddy J, et al. The unique and cooperative roles of the Grainy head-like transcription factors in epidermal development reflect unexpected target gene specificity. Dev Biol. 2011;349(2):512-522. doi:10.1016/j.ydbio.2010.11.011
3. Aladdin Scientific. Recombinant Human GRHL3 Protein, SKU rp367844. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.