Recombinant Human IFT57 Protein: Key Facts & FAQs

Recombinant Human IFT57 Protein is a key research reagent enabling scientists to study the IFT-B intraflagellar transport complex, cilia assembly, and ciliopathy-related molecular mechanisms in vitro and in cell-based assays.

Product Name

Recombinant Human IFT57 Protein

SKU

rp367351

Protein Complex

IFT-B complex (intraflagellar transport complex B)

Species Origin

Human (Homo sapiens)

Primary Research Area

Cilia biology, ciliopathy, intraflagellar transport

Supplier

Aladdin Scientific

What Is IFT57 and Why Is It Important?

IFT57, also known as HIPPI (Huntingtin-Interacting Protein 1 Protein Interactor) or Che-13, is a core subunit of the IFT-B intraflagellar transport complex. Intraflagellar transport (IFT) is a conserved bidirectional motility process that moves protein cargo along the ciliary axoneme, and is essential for the assembly, maintenance, and signalling functions of cilia and flagella across eukaryotes.

IFT57 plays a structural role within the IFT-B complex, contributing to complex stability and cargo loading. Loss-of-function studies in model organisms have linked IFT57 deficiency to defective cilia formation and phenotypes associated with ciliopathies—a class of human genetic diseases affecting organs including the kidney, retina, and brain. Recombinant Human IFT57 Protein allows researchers to investigate these processes at the molecular level in cell-free or reconstituted systems.

Product Specifications and Quality

Aladdin Scientific's Recombinant Human IFT57 Protein (SKU: rp367351) is produced using recombinant expression technology to ensure batch-to-batch consistency suitable for demanding research applications. Key specification considerations for this type of recombinant protein include:

• Expression host: Typically Escherichia coli or a eukaryotic expression system; please consult the current product datasheet for confirmed host details.
• Purity: Recombinant proteins of this class are commonly supplied at >90% purity as assessed by SDS-PAGE; refer to the Certificate of Analysis (CoA) for lot-specific data.
• Tag information: The protein may carry an affinity purification tag (e.g., His-tag); confirm tag presence and position in the current datasheet.
• Formulation: Typically supplied in a buffered solution or lyophilized form; check the product page for current formulation details.

Note: Specific numerical values for molecular weight, concentration, and endotoxin level should be confirmed from the official product datasheet or Certificate of Analysis, as these may vary by lot.

Storage, Handling, and Reconstitution

Proper storage and handling are critical for maintaining the activity and integrity of recombinant proteins. General best-practice guidelines for Recombinant Human IFT57 Protein include:

• Storage temperature: Store at −20°C or −80°C as recommended on the product datasheet. Avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, prepare single-use aliquots to minimise freeze-thaw cycling.
• Reconstitution: If supplied in lyophilized form, reconstitute in the buffer specified in the datasheet (commonly sterile PBS or Tris-HCl buffer). Allow the vial to equilibrate to room temperature briefly before adding diluent, and mix gently by pipetting—avoid vortexing.
• Working concentration: Titrate the protein for each specific assay, as optimal concentrations vary by application.

Always consult the product-specific datasheet and Certificate of Analysis supplied with each lot before use.

Research Applications

Recombinant Human IFT57 Protein is suitable for a range of in vitro and biochemical research applications, including:

• Protein–protein interaction studies: As a component of the IFT-B complex, IFT57 interacts with multiple IFT-B partners (e.g., IFT25, IFT38). Recombinant IFT57 can be used in pull-down assays, co-immunoprecipitation validation, or surface plasmon resonance (SPR) experiments to map binding interfaces.
• Biochemical reconstitution: Reconstituting sub-complexes of the IFT-B machinery in vitro to study assembly hierarchy and stoichiometry.
• Antibody production and validation: As an immunogen or positive control antigen for anti-IFT57 antibody development and ELISA.
• Structural biology: As a component for crystallography or cryo-EM studies of IFT complex architecture.
• Ciliopathy disease modelling: Functional rescue or dominant-negative experiments in cell-based cilia assembly assays.

Researchers should validate the protein in their specific assay format using appropriate positive and negative controls.

Ordering and Support

Recombinant Human IFT57 Protein is available from Aladdin Scientific under SKU rp367351. To place an order or request additional technical information:

• Visit the product page on the Aladdin Scientific website and add the item to your cart, or contact your local Aladdin Scientific representative.
• Request a Certificate of Analysis (CoA) or Safety Data Sheet (SDS) through the Aladdin Scientific documentation portal or by contacting customer service.
• For bulk quantities, custom formulations, or OEM enquiries, please reach out to the Aladdin Scientific sales team directly.
• Technical support queries regarding experimental design or troubleshooting can be directed to the Aladdin Scientific technical support team.

Aladdin Scientific is committed to providing high-quality research reagents with full documentation to support reproducible science.

Frequently asked questions

What is IFT57 and what role does it play in cilia biology?

IFT57 is a core subunit of the IFT-B intraflagellar transport complex, which is essential for building and maintaining cilia and flagella in eukaryotic cells. It contributes to complex structural integrity and cargo transport along the ciliary axoneme. Defects in IFT57 have been associated with ciliopathies, genetic disorders affecting ciliated organs such as the kidney, retina, and brain.

What applications is Recombinant Human IFT57 Protein suitable for?

Recombinant Human IFT57 Protein is suitable for protein–protein interaction studies (e.g., pull-down, co-IP, SPR), in vitro biochemical reconstitution of IFT-B sub-complexes, antibody development and validation as an antigen, and structural biology studies including crystallography and cryo-EM. It may also be used as a functional reagent in cell-based cilia assembly experiments.

How should Recombinant Human IFT57 Protein be stored to maintain activity?

The protein should be stored according to the temperature recommendation on the product datasheet, typically at −20°C or −80°C. Repeated freeze-thaw cycles should be avoided; upon first thaw, the protein should be aliquoted into single-use volumes. If supplied lyophilized, reconstitute gently in the recommended buffer without vortexing.

How can I obtain a Certificate of Analysis or Safety Data Sheet for SKU rp367351?

You can request the Certificate of Analysis (CoA) or Safety Data Sheet (SDS) for Recombinant Human IFT57 Protein (rp367351) through the Aladdin Scientific documentation portal on the website, or by contacting Aladdin Scientific customer service directly. Lot-specific CoA documents confirm purity, concentration, and quality control data.

Is Recombinant Human IFT57 Protein suitable for in vivo or clinical use?

No. Recombinant Human IFT57 Protein is intended for research use only (RUO) and is not approved or validated for in vivo administration, diagnostic, or therapeutic applications in humans or animals. It should be handled by trained laboratory personnel following appropriate biosafety guidelines.

References

1. Taschner M, Bhogaraju S, Lorentzen E. Architecture and function of IFT complex proteins in ciliogenesis. Differentiation. 2012;83(2):S12-22. doi:10.1016/j.diff.2011.11.001
2. Katoh Y, Terada M, Nishijima Y, et al. Overall architecture of the intraflagellar transport (IFT)-B complex containing Cluap1/IFT38 as an essential component of the IFT-B peripheral subcomplex. J Biol Chem. 2016;291(21):10962-10975. doi:10.1074/jbc.M116.713727
3. Pazour GJ, Dickert BL, Vucica Y, et al. Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella. J Cell Biol. 2000;151(3):709-718. doi:10.1083/jcb.151.3.709

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.