Recombinant Human KRI1 Protein: FAQs & Key Facts

Recombinant Human KRI1 Protein is a recombinant form of the human KRI1 homolog protein involved in ribosome biogenesis and nuclear processes, offered by Aladdin Scientific for use in biochemical and cell biology research applications.

Product Name

Recombinant Human KRI1 Protein

SKU

rp373182

Protein Origin

Human (Homo sapiens)

Protein Type

Recombinant protein

Primary Research Area

Ribosome biogenesis, nuclear biology

Supplier Reference

absea#PP-7101

What Is KRI1 and What Is Its Biological Role?

KRI1 (KRI1 Homolog) is a conserved nuclear protein that plays an important role in ribosome biogenesis. It is involved in the processing and maturation of ribosomal RNA (rRNA), a critical step in the assembly of functional ribosomes. KRI1 was originally identified as a homolog of the yeast Kri1p protein, which is essential for 40S ribosomal subunit biogenesis. In human cells, KRI1 localizes predominantly to the nucleus and nucleolus, consistent with its role in pre-rRNA processing. Dysregulation of ribosome biogenesis factors such as KRI1 has been implicated in cellular stress responses and may have relevance to diseases involving abnormal cell proliferation.

Product Specifications and Quality

The Recombinant Human KRI1 Protein (SKU: rp373182) is produced as a recombinant protein derived from the human KRI1 gene sequence. This product is manufactured under controlled conditions to ensure consistency and reliability for research use. The product is referenced under supplier code absea#PP-7101. Researchers are advised to consult the product datasheet for detailed specifications including molecular weight, expression host, tag information, purity grade, and formulation details, as these parameters are critical for experimental design and reproducibility.

Note: Specific numerical values for purity percentage, endotoxin level, and exact molecular weight are not listed in the current source material. Please refer to the official product datasheet or contact Aladdin Scientific technical support for confirmed specifications.

Research Applications of Recombinant Human KRI1 Protein

Recombinant Human KRI1 Protein is suitable for a range of in vitro biochemical and cell biology research applications. Typical uses for recombinant nuclear proteins of this class include:

• Protein–protein interaction studies: Investigating binding partners of KRI1 within the ribosome biogenesis pathway.
• Antibody development and validation: Used as an antigen standard for generating or validating KRI1-specific antibodies.
• Functional assays: Studying the enzymatic or structural role of KRI1 in pre-rRNA processing complexes.
• Western blot positive controls: Serving as a reference standard in immunodetection experiments.

Researchers should validate suitability for each specific assay format. Contact Aladdin Scientific technical support for application-specific guidance.

Storage, Handling, and Stability

Proper storage and handling of Recombinant Human KRI1 Protein are essential to maintain protein activity and integrity. As with most recombinant proteins, the following general best practices apply:

• Store the protein at –20°C or –80°C upon receipt, as specified in the product datasheet.
• Avoid repeated freeze–thaw cycles, which can lead to protein aggregation and loss of activity. Aliquot the stock into single-use volumes where possible.
• Keep the protein on ice during experimental procedures.
• Do not vortex; mix gently by pipetting or inversion.

Important: Always refer to the specific storage conditions stated on the product datasheet for SKU rp373182, as formulation buffers and stabilizers may affect optimal storage temperature and shelf life.

Ordering and Support Information

Recombinant Human KRI1 Protein is available from Aladdin Scientific under SKU rp373182. To place an order or request a quotation, visit the Aladdin Scientific website or contact your regional sales representative. For technical inquiries regarding experimental suitability, assay optimization, or custom quantities, please reach out to the Aladdin Scientific technical support team.

When ordering, please reference the SKU number rp373182 and the supplier reference absea#PP-7101 to ensure accurate product identification. Volume discounts and institutional pricing may be available; contact the sales team for details.

Frequently asked questions

What is the biological function of the human KRI1 protein?

Human KRI1 is a nuclear protein that functions as a homolog of the yeast Kri1p and is involved in ribosome biogenesis, specifically in the processing and maturation of ribosomal RNA required for 40S ribosomal subunit assembly. It localizes predominantly to the nucleus and nucleolus in human cells.

What is the SKU for Recombinant Human KRI1 Protein at Aladdin Scientific?

The Recombinant Human KRI1 Protein is available at Aladdin Scientific under SKU rp373182, with the supplier reference number absea#PP-7101. Please use this SKU when placing orders to ensure correct product identification.

How should Recombinant Human KRI1 Protein be stored to maintain stability?

As a general best practice for recombinant proteins, the product should be stored at –20°C or –80°C, avoiding repeated freeze–thaw cycles by aliquoting into single-use volumes. Always refer to the specific storage instructions on the product datasheet for SKU rp373182 for confirmed conditions.

What research applications is Recombinant Human KRI1 Protein suitable for?

Recombinant Human KRI1 Protein is suitable for applications such as protein–protein interaction studies, antibody development and validation, functional assays related to ribosome biogenesis, and as a positive control in Western blot experiments. Researchers should validate suitability for each specific assay.

Is detailed specification data such as purity and endotoxin levels available for rp373182?

Detailed specification data including purity percentage, endotoxin levels, exact molecular weight, and formulation are not listed in the general product listing. Please consult the official product datasheet for SKU rp373182 available on the Aladdin Scientific website, or contact technical support for confirmed specifications.

References

1. Wegierski T, et al. Bms1p, a G-domain-containing protein, associates with Rcl1p and is required for 18S rRNA biogenesis in yeast. RNA. 2001. [KRI1 yeast homolog context — needs review for direct KRI1 relevance]
2. UniProt entry for human KRI1 homolog (Homo sapiens) — recommended for confirmed gene/protein details.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.