Recombinant Human KRTAP27-1 Protein: Protocol & Usage Guide

Recombinant Human KRTAP27-1 Protein is a research-grade recombinant protein designed for use in biochemical and cell-biological studies of keratin-associated protein function, hair fiber assembly, and related structural protein interactions.

Product Name

Recombinant Human KRTAP27-1 Protein

SKU

rp369052

Protein Family

Keratin-Associated Protein (KRTAP)

Species Origin

Human (Homo sapiens)

Application

Biochemical assays, protein interaction studies, structural biology research

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles

Background: KRTAP27-1 and Keratin-Associated Proteins

Keratin-associated proteins (KRTAPs) are a large family of structural proteins that interact with keratin intermediate filaments to form the rigid, cross-linked matrix of the hair shaft cortex. KRTAP27-1 (Keratin-Associated Protein 27-1) is a member of this family encoded by the KRTAP27-1 gene in humans. KRTAPs are characterized by high cysteine or glycine/tyrosine content, which facilitates disulfide bond formation and tight packing within the hair fiber. Research into KRTAP proteins contributes to our understanding of hair texture, structural integrity, and hereditary hair disorders. Recombinant Human KRTAP27-1 Protein enables in vitro investigation of these interactions without the need for tissue extraction.

Reconstitution Protocol

Follow these steps to reconstitute Recombinant Human KRTAP27-1 Protein (rp369052) prior to use:

1. Equilibrate the vial: Allow the lyophilized protein vial to equilibrate to room temperature (approximately 15–25°C) for 10–15 minutes before opening to minimize condensation.
2. Prepare reconstitution buffer: Use sterile, deionized water or the buffer recommended in the specific product datasheet. Commonly, PBS (pH 7.4) containing a carrier protein (e.g., 0.1% BSA) is suitable. Consult the product certificate of analysis for buffer-specific guidance.
3. Add reconstitution buffer: Gently pipette the reconstitution buffer down the side of the vial. Do not add directly onto the lyophilized cake to prevent foaming or denaturation.
4. Mix gently: Swirl the vial slowly or allow it to sit for 5 minutes at room temperature. Do NOT vortex, as shear forces can denature the protein.
5. Inspect the solution: The solution should appear clear to slightly opalescent. Discard if particulate matter is observed after gentle mixing.
6. Aliquot immediately: Divide into single-use aliquots to avoid repeated freeze-thaw cycles.

Note: Specific reconstitution volume and concentration targets should be confirmed against the product-specific datasheet or certificate of analysis provided with each lot.

Storage and Stability

Proper storage is critical for maintaining the activity and integrity of Recombinant Human KRTAP27-1 Protein:

• Lyophilized form: Store at -20°C upon receipt. When stored correctly, lyophilized protein is stable for up to 12 months. Keep desiccated.
• Reconstituted form: Aliquots may be stored at -80°C for extended periods (up to 6 months is typical for recombinant proteins; verify with product datasheet). Short-term storage (up to 1 week) may be acceptable at -20°C.
• Avoid freeze-thaw cycles: Each freeze-thaw cycle risks protein aggregation and activity loss. Single-use aliquots are strongly recommended.
• Working solutions: Keep on ice during experiments. Do not leave at room temperature for extended periods.

Disclaimer: Stability data above reflect general best practices for recombinant proteins. Confirm lot-specific stability with the certificate of analysis.

Experimental Applications and Assay Tips

Recombinant Human KRTAP27-1 Protein is suitable for multiple research applications, including but not limited to:

• Protein-protein interaction studies: Use in pull-down or co-immunoprecipitation assays to investigate binding partners among keratin intermediate filament proteins.
• Western blot (WB) standard: May serve as a positive control or molecular weight standard in immunoblotting experiments targeting KRTAP27-1.
• ELISA and immunoassay development: Suitable as a coating or standard antigen for the development and validation of KRTAP27-1-specific immunoassays.
• Structural and biophysical studies: Can be used in circular dichroism (CD), dynamic light scattering (DLS), or other biophysical assays to characterize protein conformation.
• Antibody production and validation: Useful as an immunogen or validation reagent for raising and testing antibodies against KRTAP27-1.

General tips: Optimize protein concentration for each assay system. Include appropriate negative controls. When using in cell-based assays, verify that the protein formulation is compatible with cell culture conditions and free from cytotoxic additives.

Quality, Safety, and Ordering Information

Quality: Recombinant Human KRTAP27-1 Protein (rp369052) from Aladdin Scientific is manufactured and quality-controlled to research-reagent standards. Each lot is accompanied by a certificate of analysis detailing purity, concentration, and endotoxin levels where applicable. Researchers are advised to review lot-specific documentation prior to use.

Safety: This product is intended for research use only (RUO) and is not approved for diagnostic, therapeutic, or veterinary applications. Standard laboratory safety practices should be followed when handling recombinant proteins. Consult the Safety Data Sheet (SDS) for hazard information.

Ordering: To order Recombinant Human KRTAP27-1 Protein, reference SKU rp369052 on the Aladdin Scientific website or contact your local Aladdin Scientific representative. For bulk quantities or custom formulations, please inquire with our technical sales team.

Frequently asked questions

What buffer should I use to reconstitute Recombinant Human KRTAP27-1 Protein?

Sterile deionized water or PBS (pH 7.4) supplemented with a carrier protein such as 0.1% BSA is generally recommended for reconstituting recombinant proteins. Always consult the product-specific datasheet or certificate of analysis provided with your lot of rp369052 for the most accurate guidance.

How many freeze-thaw cycles can Recombinant Human KRTAP27-1 Protein withstand?

As with most recombinant proteins, repeated freeze-thaw cycles should be avoided as they can lead to protein aggregation and loss of activity. We strongly recommend aliquoting the reconstituted protein into single-use volumes and storing them at -80°C to minimize freeze-thaw exposure.

Is Recombinant Human KRTAP27-1 Protein suitable for cell-based assays?

Recombinant Human KRTAP27-1 Protein may be applicable in cell-based assays; however, researchers should verify compatibility of the protein formulation with cell culture conditions and confirm that endotoxin levels are within acceptable limits for the intended assay. Consult the certificate of analysis for endotoxin data.

What is the biological function of KRTAP27-1?

KRTAP27-1 belongs to the keratin-associated protein (KRTAP) family, which plays a structural role in hair fiber formation by interacting with keratin intermediate filaments in the hair shaft cortex. These interactions contribute to the mechanical strength and texture of hair. Recombinant KRTAP27-1 enables in vitro study of these structural protein interactions.

How should I confirm the identity and purity of my KRTAP27-1 protein lot?

Each lot of Recombinant Human KRTAP27-1 Protein (rp369052) from Aladdin Scientific is accompanied by a certificate of analysis (CoA) that includes purity assessment (typically by SDS-PAGE), concentration, and other quality parameters. Contact Aladdin Scientific customer service if you require additional lot-specific documentation.

References

1. Rogers MA, Langbein L, Praetzel-Wunder S, Schirmacher P, Schweizer J. Human hair keratin-associated proteins (KAPs). International Review of Cytology. 2006;251:209-263.
2. Shimomura Y, Ito M. Human hair keratin-associated proteins. Journal of Investigative Dermatology Symposium Proceedings. 2005;10(3):230-233.
3. Aladdin Scientific. Recombinant Human KRTAP27-1 Protein, SKU rp369052. Product Datasheet. Aladdin Scientific.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.